Detailed information NTG0040430

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: ALIDE2_RS04920
• Genome accession: NC_015422
• Coordinates: 1028678..1029802 (-)
• Length: 374 a.a.
• NCBI ID: WP_013721532.1
• Uniprot ID: F4G9H7
• Organism: Alicycliphilus denitrificans K601
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1023678..1034802

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ALIDE2_RS04895 (Alide2_0979)	-	1023771..1024937 (-)	1167	WP_013721528.1	mandelate racemase/muconate lactonizing enzyme family protein	-
ALIDE2_RS04900 (Alide2_0980)	-	1024963..1025667 (-)	705	WP_013520255.1	SDR family NAD(P)-dependent oxidoreductase	-
ALIDE2_RS04905 (Alide2_0981)	-	1025657..1026622 (-)	966	WP_013721529.1	Bug family tripartite tricarboxylate transporter substrate binding protein	-
ALIDE2_RS04910 (Alide2_0982)	-	1026654..1027592 (-)	939	WP_013721530.1	dihydrodipicolinate synthase family protein	-
ALIDE2_RS04915 (Alide2_0983)	-	1027711..1028664 (+)	954	WP_013721531.1	LysR family transcriptional regulator	-
ALIDE2_RS04920 (Alide2_0984)	pilA	1028678..1029802 (-)	1125	WP_013721532.1	signal recognition particle-docking protein FtsY	Machinery gene
ALIDE2_RS04925 (Alide2_0985)	-	1029832..1031262 (+)	1431	WP_013721533.1	M16 family metallopeptidase	-
ALIDE2_RS04930 (Alide2_0986)	-	1031276..1032634 (+)	1359	WP_013520249.1	M16 family metallopeptidase	-
ALIDE2_RS04935 (Alide2_0987)	rsmD	1032631..1033221 (+)	591	WP_013520248.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
ALIDE2_RS04940 (Alide2_0988)	coaD	1033349..1033837 (+)	489	WP_013520247.1	pantetheine-phosphate adenylyltransferase	-
ALIDE2_RS04945 (Alide2_0989)	-	1033993..1034307 (-)	315	WP_013520246.1	BON domain-containing protein	-

Sequence

Protein

• Length: 374 a.a.
• Molecular weight: 38981.59 Da
• Isoelectric Point: 6.5134

>NTDB_id=40430 ALIDE2_RS04920 WP_013721532.1 1028678..1029802(-) (pilA) [Alicycliphilus denitrificans K601]
MFSFFKKKSPSPAEPSSAPVPDAASAPAADAPTGGGALGWLRKSFGAQDAAAPQAAPAADEAPPAVPEPAPKPKGTRQGW
MERLKSGLRKTGGSIATVFTGTQINDALYEELEDALLMADTGVKATQHLLADLRRRVKEAKATEPAAVKALLADALADLL
RPLEKPLVIGAHQPTVIMVAGVNGAGKTTSIGKLTRHLADHGESVLLAAADTFRAAAREQLGVWATRNTVEIISQEGGDP
AAVSFDAVTAGRARGKDVVLVDTAGRLPTQLHLMEELKKIKRVVTKADATAPHEVLLVIDGNTGQNALAQVRAFDDALSL
TGLIVTKLDGTAKGGVLAAIAQERPVPVYFIGVGEKVEDLETFDAREFAQALLA

Nucleotide

• Length: 1125 bp

>NTDB_id=40430 ALIDE2_RS04920 WP_013721532.1 1028678..1029802(-) (pilA) [Alicycliphilus denitrificans K601]
ATGTTCAGTTTTTTCAAGAAGAAATCCCCCTCCCCCGCCGAGCCGTCCAGCGCGCCGGTGCCGGATGCCGCCAGCGCCCC
CGCGGCCGACGCGCCCACGGGCGGCGGCGCGCTCGGCTGGCTGCGCAAGTCCTTCGGCGCGCAGGACGCCGCCGCACCGC
AGGCCGCGCCTGCGGCGGACGAGGCCCCGCCCGCCGTGCCCGAGCCCGCCCCCAAGCCCAAAGGCACGCGCCAGGGCTGG
ATGGAGCGCCTCAAGTCCGGCCTGCGCAAGACCGGCGGCAGCATCGCCACCGTCTTCACGGGCACGCAGATCAACGACGC
GCTGTACGAGGAGCTCGAGGATGCCCTGCTCATGGCCGACACCGGCGTGAAGGCCACGCAGCACCTGCTGGCCGACCTCA
GGCGCCGCGTGAAGGAGGCCAAGGCCACCGAGCCCGCCGCCGTCAAGGCCCTGCTGGCCGACGCGCTGGCCGACCTGCTG
CGCCCGCTGGAAAAGCCCCTGGTCATCGGCGCGCATCAGCCCACCGTGATCATGGTGGCCGGCGTGAACGGCGCGGGCAA
GACCACCTCCATCGGCAAGCTCACCAGGCACCTGGCCGACCACGGCGAGAGCGTGCTGCTGGCCGCGGCCGACACCTTCC
GCGCCGCGGCGCGCGAGCAGCTCGGCGTCTGGGCCACGCGCAACACGGTGGAGATCATCAGCCAGGAAGGGGGCGACCCC
GCCGCCGTGAGCTTCGACGCCGTCACCGCCGGCCGCGCGCGCGGCAAGGACGTGGTGCTGGTGGACACGGCCGGGCGCCT
GCCCACGCAGCTGCACCTGATGGAGGAGCTCAAGAAGATCAAGCGCGTCGTGACCAAGGCCGACGCCACGGCCCCGCACG
AGGTGCTGCTGGTGATCGACGGCAACACCGGCCAGAACGCCCTGGCCCAGGTGCGCGCGTTCGACGACGCGCTCTCGCTC
ACCGGCCTCATCGTCACCAAGCTCGACGGCACGGCCAAGGGCGGCGTGCTGGCCGCCATCGCGCAGGAGCGGCCCGTGCC
CGTCTACTTCATCGGCGTGGGCGAGAAGGTGGAAGACCTGGAGACCTTCGACGCGCGCGAGTTCGCGCAGGCGCTGCTGG
CCTGA

Domains

Predicted by InterproScan.

• SRP54-type protein, helical bundle domain (SRP54_N) (93-156)

• SRP54-type protein, GTPase domain (SRP54) (174-373)

3D structure

• AlphaFold DB: F4G9H7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	50	100	0.556