Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   GD442_RS20805 Genome accession   NZ_CP046398
Coordinates   4100411..4101454 (-) Length   347 a.a.
NCBI ID   WP_159365189.1    Uniprot ID   A0AAE6R116
Organism   Bacillus sp. A260     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4095411..4106454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GD442_RS20770 (GD442_20775) - 4095925..4096713 (+) 789 WP_159365188.1 hypothetical protein -
  GD442_RS20775 (GD442_20780) - 4096860..4098029 (+) 1170 WP_153598528.1 site-specific integrase -
  GD442_RS20780 (GD442_20785) - 4098177..4098359 (-) 183 Protein_4118 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GD442_RS20785 (GD442_20790) - 4098329..4098631 (-) 303 WP_000829468.1 hypothetical protein -
  GD442_RS20790 (GD442_20795) comGD 4098624..4099079 (-) 456 WP_000813141.1 competence type IV pilus minor pilin ComGD -
  GD442_RS20795 (GD442_20800) comGC 4099076..4099375 (-) 300 WP_001178687.1 competence type IV pilus major pilin ComGC -
  GD442_RS20800 (GD442_20805) comGB 4099387..4100418 (-) 1032 WP_000475187.1 competence type IV pilus assembly protein ComGB -
  GD442_RS20805 (GD442_20810) comGA 4100411..4101454 (-) 1044 WP_159365189.1 competence type IV pilus ATPase ComGA Machinery gene
  GD442_RS20810 (GD442_20815) - 4101660..4102355 (+) 696 WP_000434125.1 helix-turn-helix domain-containing protein -
  GD442_RS20815 (GD442_20820) - 4102481..4102723 (+) 243 WP_000440716.1 DUF2626 domain-containing protein -
  GD442_RS20820 (GD442_20825) - 4102831..4104225 (+) 1395 WP_001094345.1 L-cystine transporter -
  GD442_RS20825 (GD442_20830) - 4104304..4104695 (-) 392 Protein_4127 hypothetical protein -
  GD442_RS20830 (GD442_20835) - 4104783..4104998 (-) 216 WP_001008327.1 DUF3912 family protein -
  GD442_RS20835 (GD442_20840) - 4105251..4105562 (+) 312 WP_001093239.1 hypothetical protein -
  GD442_RS20840 (GD442_20845) - 4105597..4106085 (-) 489 WP_159365190.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39121.58 Da        Isoelectric Point: 8.7212

>NTDB_id=403920 GD442_RS20805 WP_159365189.1 4100411..4101454(-) (comGA) [Bacillus sp. A260]
MNGIEIFANAILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGLLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEAGGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=403920 GD442_RS20805 WP_159365189.1 4100411..4101454(-) (comGA) [Bacillus sp. A260]
ATGAATGGGATTGAAATTTTTGCGAATGCAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTTCAACTACGTATAGGAAAAGATTTAATGACGAGACAGTGTATTGAAAAGGAATTTGGAG
AAAAACTTGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGCGAGAAACGGAAGCCACAGAATGGTTTGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTTGTGTTTACTGGACCGACTGGATCTGGGAAGACGACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACACTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGCTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAGGCTAGCATATATGAGCTACTATATGGATATGAGTTAAAACAAGCGCTTAAAGAAGCTGGTGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.62

100

0.556
  pilB Haemophilus influenzae 86-028NP

40.062

92.795

0.372

Multiple sequence alignment