Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   HW371_RS04030 Genome accession   NZ_CP058220
Coordinates   821083..821892 (+) Length   269 a.a.
NCBI ID   WP_001305093.1    Uniprot ID   -
Organism   Escherichia coli strain 83972     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 816083..826892
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HW371_RS04025 (HW371_03980) sslE 816397..820953 (+) 4557 WP_001034480.1 lipoprotein metalloprotease SslE -
  HW371_RS04030 (HW371_03985) pilD 821083..821892 (+) 810 WP_001305093.1 prepilin peptidase PppA Machinery gene
  HW371_RS04035 (HW371_03990) gspS2 821958..822368 (+) 411 WP_001305092.1 type II secretion system pilot lipoprotein GspS-beta -
  HW371_RS04040 (HW371_03995) gspC 822386..823345 (+) 960 WP_001305091.1 type II secretion system protein GspC -
  HW371_RS04045 (HW371_04000) gspD 823375..825435 (+) 2061 WP_000498847.1 type II secretion system secretin GspD -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 29448.04 Da        Isoelectric Point: 8.3801

>NTDB_id=403750 HW371_RS04030 WP_001305093.1 821083..821892(+) (pilD) [Escherichia coli strain 83972]
MLFDVFQQYSAGMPILATVGGLIIGSFLNVVIWRYPIMLRQQMAEFHGEMPSTQSKINLALPRSHCPHCQQTIRVRDNIP
LLSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASVIDLDNQWLPDVFTQGVLWT
GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGWVGPLSLPNVALIASCCGLIYAV
ITKRGSTTLPFGPCLSLGGIATLYLQALF

Nucleotide


Download         Length: 810 bp        

>NTDB_id=403750 HW371_RS04030 WP_001305093.1 821083..821892(+) (pilD) [Escherichia coli strain 83972]
ATGCTTTTTGATGTTTTTCAGCAATACTCCGCGGGGATGCCCATACTGGCAACCGTCGGAGGATTGATCATCGGCAGTTT
TTTGAATGTGGTGATTTGGCGTTACCCCATCATGCTGCGCCAACAAATGGCGGAGTTTCACGGTGAAATGCCGAGTACGC
AGTCAAAAATAAACCTGGCGCTGCCACGCTCGCACTGTCCGCATTGCCAGCAGACCATCCGCGTTCGTGACAATATTCCG
CTGCTCTCATGGTTGATGCTCAAAGGGCGCTGCCGCGACTGTCAGGCGAAAATCAGCAAGCGTTATCCGCTGGTGGAGTT
ATTGACGGCACTCGCTTTTTTGCTGGCGAGTCTGGTGTGGCCAGAAAGTGGATGGGGGCTGGCGGTGATGATATTATCCG
CCTGGCTGATTGCCGCGAGCGTCATTGACCTCGATAACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACC
GGGCTGATTGCGGCATGGGCGCAACAGAGTCCGTTAACGCTACAAGACGCAGTTACCGGCGTCCTGGTGGGGTTTATCAC
TTTTTACTCCCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTACTTTTCGCTG
CGTTAGGTGGCTGGGTGGGGCCGTTGTCGCTACCCAATGTTGCTTTAATCGCATCATGCTGCGGCCTGATATATGCCGTT
ATTACAAAAAGAGGATCAACCACACTGCCTTTTGGACCGTGTTTAAGTCTGGGCGGTATAGCAACACTTTATCTACAGGC
ATTGTTTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(130-239)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

45.318

99.257

0.45
  pilD Vibrio campbellii strain DS40M4

41.328

100

0.416
  pilD Acinetobacter nosocomialis M2

38.113

98.513

0.375
  pilD Neisseria gonorrhoeae MS11

39.841

93.309

0.372
  pilD Acinetobacter baumannii D1279779

37.736

98.513

0.372