Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   PSEBR_RS25895 Genome accession   NC_015379
Coordinates   5869831..5871420 (+) Length   529 a.a.
NCBI ID   WP_003205701.1    Uniprot ID   F2KLF0
Organism   Pseudomonas brassicacearum subsp. brassicacearum NFM421     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5864831..5876420
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSEBR_RS25875 (PSEBR_a4840) pgeF 5866630..5867355 (-) 726 WP_013694236.1 peptidoglycan editing factor PgeF -
  PSEBR_RS25880 (PSEBR_a4841) rluD 5867352..5868314 (-) 963 WP_003205696.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  PSEBR_RS25885 (PSEBR_a4842) - 5868465..5869481 (+) 1017 WP_003205698.1 outer membrane protein assembly factor BamD -
  PSEBR_RS25890 (PSEBR_cmegm118) - 5869608..5869841 (+) 234 WP_013694237.1 PP0621 family protein -
  PSEBR_RS25895 (PSEBR_a4843) pilS 5869831..5871420 (+) 1590 WP_003205701.1 two-component system sensor histidine kinase NtrB Regulator
  PSEBR_RS25900 (PSEBR_a4844) pilR 5871417..5872763 (+) 1347 WP_003205703.1 sigma-54-dependent transcriptional regulator Regulator
  PSEBR_RS25905 (PSEBR_a4845) - 5872855..5873220 (-) 366 WP_003205705.1 DUF6124 family protein -
  PSEBR_RS25915 (PSEBR_a4846) thiO 5874437..5875537 (-) 1101 WP_003205709.1 glycine oxidase ThiO -
  PSEBR_RS25920 (PSEBR_a4847) - 5875593..5875994 (-) 402 WP_003205711.1 type IV pilin protein -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58937.74 Da        Isoelectric Point: 7.3882

>NTDB_id=40279 PSEBR_RS25895 WP_003205701.1 5869831..5871420(+) (pilS) [Pseudomonas brassicacearum subsp. brassicacearum NFM421]
MSAEASSPREKQAQRLLRLYHLYRLSIGITLVLLISSKLDNRLLEFANDDLLRSGSWLYLVLNILLVVFLENTRRPARLF
GLALTDVLLLSWLFFAAGGVPSAIGNLLIVSVAIGNTLLRSRVGLLIAAVATIGIVGSTFFLGLSDSNRPSSYLQAGTLG
ALCFAAALLVQGLTRRLEASETLAEQRASEVIGLEALNALILQRMRTGILVLDRERRVQLANESALNLLGMHDLVGQRID
DYCTALVERLQLWLNNPSLRPPSLTVTGTGLTLQPSFIALGHHDQQQLLVFLEDLAQVAQQAQQLKLASLGRLTAGIAHE
IRNPLGAISHAAQLLRESEELNNADRRLTQIIQDHSQRMNRVIENVLQLSRRQQTTPQRLDLRTWLDQFVRQARESMAEH
QHLHLSIDPGDYTTLMDPDQLTQVLDNLLRNAWRHSAMTHEQAEAWLKLFIDPHSQLSTLDIIDNGPGVTPEQQAHLFEP
FFTTSSQGTGLGLYLSRELCESNQARLDFKPRQGGGCFRITFAHGRKQI

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=40279 PSEBR_RS25895 WP_003205701.1 5869831..5871420(+) (pilS) [Pseudomonas brassicacearum subsp. brassicacearum NFM421]
GTGAGCGCTGAGGCCTCAAGCCCACGCGAAAAACAGGCGCAGCGCCTGCTGCGTCTCTATCACCTGTATCGCCTGAGCAT
CGGCATCACCCTGGTGCTGCTGATCTCCAGCAAACTGGACAACCGCCTGCTGGAGTTCGCCAATGACGACCTGCTGCGCA
GTGGCAGTTGGTTGTACCTGGTGCTGAATATCCTGCTGGTGGTGTTCCTTGAGAACACCCGCCGCCCTGCCCGGCTGTTC
GGCCTGGCCCTGACTGACGTGCTGCTGCTCTCGTGGCTGTTCTTCGCCGCGGGCGGTGTGCCCAGTGCCATCGGCAACCT
GCTCATTGTCTCGGTGGCCATCGGCAATACGCTGTTGCGAAGCCGGGTCGGCCTGTTGATTGCCGCTGTCGCCACGATCG
GTATCGTCGGGTCGACTTTTTTCCTCGGCCTCAGCGACTCAAACCGTCCCAGCAGTTATCTGCAAGCCGGCACCCTGGGG
GCACTGTGTTTTGCCGCCGCCCTGCTGGTACAGGGCCTGACCCGGCGACTGGAAGCCAGTGAAACCCTGGCCGAGCAACG
GGCCAGCGAGGTAATCGGCCTCGAAGCGCTCAACGCACTGATCCTGCAACGCATGCGCACCGGCATCCTGGTGCTCGACC
GCGAACGGCGCGTGCAATTGGCCAACGAAAGCGCCTTGAACCTGCTGGGCATGCACGACCTGGTCGGCCAGCGAATCGAT
GACTATTGCACCGCCCTGGTCGAACGCCTGCAATTGTGGCTGAACAACCCCAGCCTGCGCCCCCCCAGCCTGACCGTGAC
CGGCACAGGCCTGACCCTGCAACCGAGCTTCATCGCCTTGGGGCATCACGATCAGCAGCAGTTGCTGGTATTCCTTGAGG
ACCTGGCCCAAGTTGCCCAGCAGGCCCAGCAACTGAAACTCGCGTCCCTCGGGCGCCTCACTGCTGGCATCGCCCATGAA
ATCCGCAACCCCTTGGGCGCCATCAGCCACGCAGCGCAATTACTGCGCGAATCCGAGGAACTGAACAACGCGGATCGGCG
TCTGACGCAGATTATTCAAGATCACTCCCAACGAATGAATCGCGTCATTGAAAACGTCCTGCAGCTGTCTCGCCGCCAGC
AAACCACTCCCCAACGCCTCGACCTGCGGACCTGGCTCGACCAGTTCGTCCGCCAGGCCCGCGAGAGCATGGCCGAGCAC
CAGCACTTGCATCTGAGCATCGACCCGGGCGACTACACCACGCTGATGGACCCCGACCAGTTGACCCAGGTACTCGACAA
CCTGCTGCGCAACGCCTGGCGCCACAGCGCCATGACCCACGAACAGGCCGAGGCCTGGCTGAAACTGTTCATCGATCCCC
ACAGCCAGCTGTCCACACTGGACATCATCGACAACGGCCCCGGTGTAACACCCGAGCAGCAGGCACATTTGTTCGAACCC
TTCTTTACCACCAGCAGCCAGGGCACCGGCCTTGGGCTCTATCTGTCCCGTGAGCTGTGCGAAAGCAACCAGGCCCGCCT
AGACTTCAAACCACGCCAAGGCGGCGGTTGCTTTCGCATCACCTTTGCTCACGGACGGAAACAGATTTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-522)

His Kinase A (phospho-acceptor) domain (HisKA)

(309-374)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F2KLF0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

60.038

99.811

0.599

Multiple sequence alignment