Detailed information NTG0402406

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: FOB21_RS08300
• Genome accession: NZ_CP046296
• Coordinates: 1450256..1451116 (+)
• Length: 286 a.a.
• NCBI ID: WP_004281094.1
• Uniprot ID: N9QGW2
• Organism: Acinetobacter lwoffii strain FDAARGOS_552
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1445256..1456116

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FOB21_RS08280 (FOB21_08280)	secG	1445874..1446203 (-)	330	WP_004281101.1	preprotein translocase subunit SecG	-
FOB21_RS08285 (FOB21_08285)	tpiA	1446217..1447008 (-)	792	WP_004281100.1	triose-phosphate isomerase	-
FOB21_RS08290 (FOB21_08290)	pilB	1447286..1448995 (+)	1710	WP_005265509.1	type IV-A pilus assembly ATPase PilB	Machinery gene
FOB21_RS08295 (FOB21_08295)	pilC	1449027..1450256 (+)	1230	WP_004281096.1	type II secretion system F family protein	Machinery gene
FOB21_RS08300 (FOB21_08300)	pilD	1450256..1451116 (+)	861	WP_004281094.1	prepilin peptidase	Machinery gene
FOB21_RS08305 (FOB21_08305)	coaE	1451118..1451717 (+)	600	WP_004281093.1	dephospho-CoA kinase	-
FOB21_RS08310 (FOB21_08310)	-	1451739..1452662 (-)	924	WP_004644926.1	DMT family transporter	-
FOB21_RS08315 (FOB21_08315)	rlmB	1452669..1453418 (-)	750	WP_004281091.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
FOB21_RS08320 (FOB21_08320)	-	1453553..1453876 (-)	324	WP_004281089.1	pyrimidine/purine nucleoside phosphorylase	-
FOB21_RS08325 (FOB21_08325)	recN	1454319..1455980 (+)	1662	WP_121979630.1	DNA repair protein RecN	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 32010.35 Da
• Isoelectric Point: 8.0624

>NTDB_id=402406 FOB21_RS08300 WP_004281094.1 1450256..1451116(+) (pilD) [Acinetobacter lwoffii strain FDAARGOS_552]
MHDFLQYFIQNPTALYIAVGLFSLCIGSFLNVVIYRTPKMMEQEWRTDCQMFLHPEQPVIDETKLSLSKPASACPKCHQP
IRWYQNIPVISWLVLRGKCSNCQNPISIRYPLVEILTAVCALLVVAVFGPTLQMLFGLILTYVLIALTFIDFDTQLLPDR
YTLPLAALGLGVNSYALYTLPGAAIWGYIIGFLCLWVVYYAFKIVTGKEGMGYGDFKLLAALGAWMGPLLLPLIVLLSSL
VGAIIGIILLKVRKENQPFAFGPYIAIAGWIAFLWGEQIMKVYLGQ

Nucleotide

• Length: 861 bp

>NTDB_id=402406 FOB21_RS08300 WP_004281094.1 1450256..1451116(+) (pilD) [Acinetobacter lwoffii strain FDAARGOS_552]
ATGCACGATTTTCTTCAATATTTTATTCAAAACCCAACGGCGTTGTATATCGCAGTTGGGCTTTTTAGTTTATGTATCGG
CAGCTTTCTGAATGTCGTGATTTACCGCACTCCCAAGATGATGGAACAGGAATGGCGTACTGATTGCCAGATGTTCCTGC
ATCCGGAACAGCCGGTGATTGATGAAACTAAGTTAAGTTTAAGCAAACCCGCTTCAGCTTGCCCCAAGTGTCATCAGCCA
ATCCGCTGGTACCAGAATATTCCCGTCATTAGCTGGCTGGTCCTGCGCGGAAAATGTAGCAACTGCCAGAATCCGATCAG
CATCCGTTATCCTTTGGTTGAAATTCTGACGGCAGTTTGTGCACTCCTTGTCGTAGCAGTATTTGGCCCTACCCTACAAA
TGCTGTTTGGCCTGATCCTGACTTATGTGCTGATCGCACTCACTTTTATTGATTTTGACACCCAGTTATTGCCTGACCGT
TATACTTTACCCTTGGCCGCGCTAGGTTTGGGGGTAAATAGTTATGCACTTTATACCTTACCCGGTGCTGCCATCTGGGG
CTATATTATTGGTTTCCTGTGTCTGTGGGTGGTGTATTATGCATTTAAAATCGTTACCGGCAAGGAAGGTATGGGCTATG
GCGATTTTAAATTGCTTGCCGCCCTGGGTGCCTGGATGGGGCCTTTATTACTGCCTTTAATTGTGCTGCTGTCTTCTCTG
GTTGGTGCGATTATCGGGATTATCCTACTGAAAGTCCGCAAGGAAAATCAGCCTTTTGCCTTTGGTCCCTATATCGCGAT
TGCCGGCTGGATTGCTTTTTTGTGGGGCGAGCAGATTATGAAAGTGTATTTAGGTCAATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-127)

• Type IV leader peptidase family (Peptidase_A24) (139-247)

3D structure

• AlphaFold DB: N9QGW2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter nosocomialis M2	80	99.65	0.797
pilD	Acinetobacter baumannii D1279779	79.649	99.65	0.794
pilD	Vibrio cholerae strain A1552	51.237	98.951	0.507
pilD	Vibrio campbellii strain DS40M4	49.826	100	0.5
pilD	Neisseria gonorrhoeae MS11	46.909	96.154	0.451