Detailed information NTG0402153

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: Vspart_RS02695
• Genome accession: NZ_CP046268
• Coordinates: 587776..588648 (-)
• Length: 290 a.a.
• NCBI ID: WP_182288159.1
• Uniprot ID: -
• Organism: Vibrio spartinae strain 3.6
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 582776..593648

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Vspart_RS02655 (Vspart_00540)	-	582968..583717 (-)	750	WP_182288157.1	putative DNA-binding domain-containing protein	-
Vspart_RS02660 (Vspart_00541)	-	583701..584651 (-)	951	WP_182288158.1	DUF692 domain-containing protein	-
Vspart_RS02665 (Vspart_00542)	-	584667..584942 (-)	276	WP_074372746.1	DUF2282 domain-containing protein	-
Vspart_RS22095 (Vspart_00543)	-	585028..585225 (-)	198	WP_205410487.1	zf-HC2 domain-containing protein	-
Vspart_RS02675 (Vspart_00544)	-	585222..585848 (-)	627	WP_074372748.1	RNA polymerase factor sigma-70	-
Vspart_RS02680	yacG	586107..586310 (-)	204	WP_074372749.1	DNA gyrase inhibitor YacG	-
Vspart_RS02685 (Vspart_00545)	zapD	586393..587133 (-)	741	WP_074372750.1	cell division protein ZapD	-
Vspart_RS02690 (Vspart_00546)	coaE	587166..587774 (-)	609	WP_074372751.1	dephospho-CoA kinase	-
Vspart_RS02695 (Vspart_00547)	pilD	587776..588648 (-)	873	WP_182288159.1	prepilin peptidase	Machinery gene
Vspart_RS02700 (Vspart_00548)	-	588755..589981 (-)	1227	WP_074372753.1	type II secretion system F family protein	-
Vspart_RS02705 (Vspart_00549)	-	589978..591726 (-)	1749	WP_182288160.1	GspE/PulE family protein	-
Vspart_RS02710 (Vspart_00550)	-	591810..592250 (-)	441	WP_074372755.1	pilin	-
Vspart_RS02715 (Vspart_00551)	-	592436..593050 (-)	615	WP_182288161.1	hypothetical protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32329.64 Da
• Isoelectric Point: 6.9975

>NTDB_id=402153 Vspart_RS02695 WP_182288159.1 587776..588648(-) (pilD) [Vibrio spartinae strain 3.6]
MDAFLIYPWLFPVFASLFGLIIGSFLNVVIYRLPQMMVTNWKQEFAEAFPEYDIPSPTKQEKISLSLPASFCPHCHHRIR
PWHNIPLFGWLLLGGKCTDCGERISIQYPLVELVTALASCIIAIHGGMTYYTLALLLFTYISIAAACIDLNQMLLPDNLT
IPLIWLGLLLSLTHINPLPLSDALIGAVAGYLSLWSIYWMFKLLTKKEGMGYGDFKYLAALGAWIGWQTLPMVILLSSVI
GIILGATLLLIRKQGNQAFPFGPSLAIAGWICLIWGEPMMSWYTSIVLGA

Nucleotide

• Length: 873 bp

>NTDB_id=402153 Vspart_RS02695 WP_182288159.1 587776..588648(-) (pilD) [Vibrio spartinae strain 3.6]
ATGGATGCATTTTTGATTTATCCTTGGTTATTTCCTGTTTTTGCGAGCCTCTTCGGGCTCATCATCGGCAGTTTTCTCAA
TGTCGTCATCTATCGTTTACCACAAATGATGGTCACAAACTGGAAGCAAGAATTTGCCGAAGCTTTCCCCGAGTACGATA
TTCCTTCCCCAACGAAGCAGGAAAAAATTTCACTCAGTCTCCCTGCATCATTCTGTCCGCACTGCCATCACCGAATCCGG
CCTTGGCATAATATTCCACTGTTTGGCTGGTTATTGCTCGGTGGCAAATGTACAGATTGTGGCGAACGGATATCGATACA
ATATCCACTGGTAGAACTCGTGACGGCACTGGCAAGCTGCATCATCGCGATTCATGGCGGTATGACCTATTATACATTGG
CATTGCTTCTCTTTACTTACATCAGCATTGCCGCGGCATGTATCGATCTCAACCAGATGTTACTTCCGGACAATTTAACC
ATCCCATTAATCTGGCTTGGGCTGTTACTATCACTGACACACATCAACCCACTCCCTTTATCCGATGCGCTGATAGGCGC
GGTGGCCGGCTATTTATCTCTCTGGTCGATTTACTGGATGTTTAAACTGCTGACCAAAAAAGAAGGCATGGGATACGGCG
ACTTTAAATATCTTGCGGCCTTAGGCGCATGGATCGGCTGGCAAACACTACCGATGGTGATTTTATTGTCCTCCGTGATT
GGCATCATTTTAGGAGCCACTCTACTGTTGATAAGAAAGCAAGGTAATCAGGCCTTTCCATTCGGCCCTTCTCTGGCGAT
TGCCGGATGGATCTGTCTCATCTGGGGGGAGCCCATGATGAGCTGGTATACATCAATCGTGTTAGGAGCATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-125)

• Type IV leader peptidase family (Peptidase_A24) (137-245)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	59.31	100	0.593
pilD	Vibrio cholerae strain A1552	58.621	100	0.586
pilD	Acinetobacter nosocomialis M2	48.889	93.103	0.455
pilD	Acinetobacter baumannii D1279779	47.407	93.103	0.441
pilD	Neisseria gonorrhoeae MS11	43.542	93.448	0.407