Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   GKC70_RS22550 Genome accession   NZ_CP046139
Coordinates   4998378..5000597 (+) Length   739 a.a.
NCBI ID   WP_342649983.1    Uniprot ID   -
Organism   Pseudomonas sp. REB1044     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4993378..5005597
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC70_RS22535 (GKC70_22290) - 4993843..4996296 (+) 2454 WP_342649982.1 penicillin-binding protein 1A -
  GKC70_RS22540 (GKC70_22295) - 4996403..4997671 (-) 1269 WP_029614302.1 malic enzyme-like NAD(P)-binding protein -
  GKC70_RS22545 (GKC70_22300) rpmE 4997995..4998210 (-) 216 WP_029614303.1 50S ribosomal protein L31 -
  GKC70_RS22550 (GKC70_22305) priA 4998378..5000597 (+) 2220 WP_342649983.1 primosomal protein N' Machinery gene
  GKC70_RS22555 (GKC70_22310) argS 5000837..5002573 (+) 1737 WP_342649984.1 arginine--tRNA ligase -
  GKC70_RS22560 (GKC70_22315) - 5002575..5003273 (+) 699 WP_039582571.1 SPOR domain-containing protein -
  GKC70_RS22565 (GKC70_22320) - 5003325..5003744 (-) 420 WP_039582570.1 TM2 domain-containing protein -
  GKC70_RS22570 (GKC70_22325) - 5003912..5004544 (+) 633 WP_342649985.1 C40 family peptidase -
  GKC70_RS22575 (GKC70_22330) pilT 5004591..5005592 (-) 1002 WP_342649986.1 PilT/PilU family type 4a pilus ATPase Machinery gene

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 82073.59 Da        Isoelectric Point: 8.3098

>NTDB_id=401587 GKC70_RS22550 WP_342649983.1 4998378..5000597(+) (priA) [Pseudomonas sp. REB1044]
MPDVILRLALPSPLRRLFDYRAPAAMVRQPLTPGMRVRVPFGRREMIGVLVEVCAQSEVPADKLKPASELLDPVSPLPPE
LFKLCLWTAHYYQHSLGDTLSWALPVLLRQGEAAEARQERFWHVAPGARLDDPRIARAPRQKEALQTLAQHPHGVAHSLL
SKLALNKDSLDLLLAKELVQVEVRRHLPPPRHEHWLAQPELPLNDEQRAAFEAVRSGFGSYHAFLLAGVTGSGKTEVYLQ
LIRETLEAGKQALILIPEINLGPQTLARFEQRFNARIALLHSAVNDRERLDAWLAARDGEADIIIGTRSALFTPMKRPGL
IIIDEEHDGSYKQQEGLRYHARDLAVVRAHQDNIPILLGSATPSLESLHNAHTGRYGLLRMNQRAGGAKPPRFMRLDVRS
LPLDSGISGPLQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWLSECPRCDARMTVHQRSGVLRCHHCGYDERLPLQC
PQCAHVDLRPVGAGTERAEERLKTLFPDYPVLRVDRDSTSRKDAMQQLFSTIQRGQPSILVGTQMLAKGHHFPRVTLVAI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIVQTHLAEHPLLVQLTEQGYFAFAEQALSERRAAGLPPFSHL
ALLRAEAHKPGQAEAFLDEACAAAERLQSEMALPGIELLGPVPAPMERRAGRFRAQLLIQASARAPLHRLIAAWLLEVEQ
LPSGRQVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=401587 GKC70_RS22550 WP_342649983.1 4998378..5000597(+) (priA) [Pseudomonas sp. REB1044]
GTGCCCGACGTCATCCTGCGCCTTGCCCTGCCCTCGCCCTTGCGTCGCCTGTTCGACTATCGGGCGCCGGCCGCCATGGT
CCGCCAGCCCCTGACCCCGGGCATGCGCGTCCGCGTGCCGTTCGGACGCCGCGAGATGATTGGCGTGCTGGTGGAAGTCT
GCGCCCAGAGCGAAGTGCCCGCCGACAAGCTCAAGCCGGCCAGCGAGCTGCTCGATCCGGTGTCGCCGCTGCCGCCTGAG
CTGTTCAAGCTGTGCCTGTGGACCGCCCACTATTATCAGCACAGTCTGGGCGACACCCTCAGTTGGGCACTGCCGGTGCT
GCTGCGCCAGGGCGAGGCGGCCGAAGCCCGGCAGGAGCGCTTTTGGCATGTCGCGCCGGGCGCGCGACTCGACGACCCGC
GCATCGCCCGCGCCCCGCGCCAGAAAGAGGCCTTGCAGACCCTCGCCCAGCATCCCCATGGCGTGGCCCACAGCCTGCTG
AGCAAGCTGGCACTGAACAAGGACAGCCTCGACCTGCTGCTGGCCAAGGAGTTGGTGCAGGTCGAGGTGCGCCGCCATCT
GCCGCCGCCGCGCCACGAGCATTGGCTGGCGCAGCCGGAGCTGCCACTCAACGATGAACAGCGGGCGGCGTTCGAAGCCG
TGCGTTCGGGCTTTGGCAGCTACCATGCCTTCCTGCTCGCCGGGGTCACCGGCAGTGGCAAGACCGAGGTCTACCTGCAA
CTGATCCGCGAAACCCTGGAGGCCGGCAAACAGGCCTTGATCCTGATTCCCGAGATCAACCTCGGCCCACAGACCCTGGC
GCGCTTCGAGCAGCGCTTCAACGCGCGCATCGCGCTGCTGCACTCGGCGGTCAACGATCGCGAGCGCCTGGACGCCTGGC
TGGCGGCCCGCGATGGCGAGGCGGACATCATCATCGGCACCCGCTCGGCGCTGTTCACGCCCATGAAGCGACCCGGCCTG
ATCATCATCGACGAAGAGCACGACGGCTCCTACAAACAGCAGGAAGGCCTGCGCTATCACGCCCGCGACCTGGCAGTGGT
GCGCGCGCACCAGGACAACATTCCCATTCTGCTGGGCTCGGCCACGCCCTCGCTGGAAAGCTTGCACAACGCCCACACCG
GCCGCTACGGTCTGTTGCGTATGAACCAGCGCGCCGGCGGTGCCAAGCCGCCGCGCTTCATGCGTCTGGATGTGCGCAGC
CTGCCGTTGGATAGCGGCATCAGCGGGCCCTTGCAACAGGCCATCGGCCAGACCCTGGCGGCCGGCCAGCAGGTGCTGGT
GTTCCTCAACCGCCGCGGCTTCGCCCCTACCTTGCTGTGCCACGACTGCGGCTGGCTGTCGGAGTGCCCGCGCTGCGATG
CGCGCATGACCGTGCATCAGCGCTCCGGCGTGCTGCGCTGTCATCACTGCGGCTACGACGAGCGTCTGCCGCTGCAATGC
CCCCAGTGCGCGCATGTCGACCTGCGGCCAGTCGGCGCCGGCACCGAGCGTGCCGAAGAGCGCCTGAAAACGCTGTTCCC
CGACTACCCGGTGCTGCGCGTGGACCGCGACAGCACCTCGCGCAAGGACGCCATGCAGCAGTTGTTCAGCACCATCCAGC
GCGGCCAGCCGAGCATCCTGGTCGGCACCCAGATGCTCGCCAAAGGCCACCATTTCCCCCGGGTGACGCTGGTGGCGATT
CTCGATGCCGACGGTGGTCTGTTCTCCGGCGACTTCCGCGCCAGCGAACGCATGGCGCAGTTGATCGTGCAGGTGGCTGG
CCGCGCCGGTCGCGCCGAGGAACCGGGAAAGGTGATCGTGCAGACCCATCTGGCCGAGCACCCGCTATTGGTACAGCTCA
CCGAGCAAGGCTATTTCGCCTTCGCCGAACAGGCCTTGAGCGAACGCCGTGCCGCCGGCCTGCCGCCGTTTTCGCACTTG
GCGCTGCTGCGCGCCGAAGCGCATAAGCCGGGCCAGGCCGAAGCCTTCCTCGACGAAGCCTGCGCCGCCGCCGAACGCCT
GCAGAGCGAGATGGCGCTGCCAGGCATCGAGCTGCTGGGCCCGGTGCCCGCCCCCATGGAGCGCCGCGCTGGCCGCTTCC
GCGCCCAGTTGCTGATCCAGGCCAGCGCCCGCGCCCCACTGCACCGGCTGATCGCCGCCTGGCTGCTGGAGGTCGAGCAA
CTGCCCTCGGGCCGCCAAGTACGCTGGTCGCTCGACGTCGACCCCGTCGACCTTTATTGA

Domains

Predicted by InterproScan.

DEAD/DEAH box helicase (DEAD)

(206-367)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)

Helicase conserved C-terminal domain (Helicase_C)

(520-592)

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.844

100

0.48

Multiple sequence alignment