Detailed information NTG0401385

Bioinformatically predicted

Overview

• Name: spo0A
• Type: Regulator
• Locus tag: GKC25_RS10580
• Genome accession: NZ_CP046128
• Coordinates: 2108113..2108916 (-)
• Length: 267 a.a.
• NCBI ID: WP_012010520.1
• Uniprot ID: W6IDW7
• Organism: Bacillus pumilus strain V17C11
• Function: activation and repression of comK; repression of rok; repression of abrB (predicted from homology) Competence regulation

Genomic Context

Location: 2103113..2113916

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GKC25_RS10565 (GKC25_10475)	-	2105333..2105569 (+)	237	WP_034661240.1	DUF2627 domain-containing protein	-
GKC25_RS10570 (GKC25_10480)	-	2105604..2106341 (-)	738	WP_034661241.1	glycerophosphodiester phosphodiesterase	-
GKC25_RS10575 (GKC25_10485)	-	2106568..2107668 (-)	1101	WP_066030680.1	Rap family tetratricopeptide repeat protein	-
GKC25_RS10580 (GKC25_10490)	spo0A	2108113..2108916 (-)	804	WP_012010520.1	sporulation transcription factor Spo0A	Regulator
GKC25_RS10585 (GKC25_10495)	spoIVB	2109202..2110479 (-)	1278	WP_034661244.1	SpoIVB peptidase	-
GKC25_RS10590 (GKC25_10500)	recN	2110678..2112411 (-)	1734	WP_106037972.1	DNA repair protein RecN	Machinery gene
GKC25_RS10595 (GKC25_10505)	argR	2112445..2112894 (-)	450	WP_034661246.1	transcriptional regulator ArgR	-
GKC25_RS10600 (GKC25_10510)	-	2113057..2113911 (-)	855	WP_034661248.1	TlyA family RNA methyltransferase	-

Sequence

Protein

• Length: 267 a.a.
• Molecular weight: 29918.44 Da
• Isoelectric Point: 6.3913

>NTDB_id=401385 GKC25_RS10580 WP_012010520.1 2108113..2108916(-) (spo0A) [Bacillus pumilus strain V17C11]
MEKIKVCVADDNRELVGLLTEYIEGQEDMEVLGVAYNGQECLTLFKDKEPDVLLLDIIMPHLDGLAVLERLRENNDMTKQ
PSVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNGTQVTHRSSSIQNSVLRNKPEPKRKNLDASITTI
IHEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTV
SMSKAKPTNSEFIAMVADRLRLEHRAS

Nucleotide

• Length: 804 bp

>NTDB_id=401385 GKC25_RS10580 WP_012010520.1 2108113..2108916(-) (spo0A) [Bacillus pumilus strain V17C11]
GTGGAGAAAATTAAAGTGTGTGTAGCTGATGACAATCGAGAGCTTGTTGGCCTTTTGACAGAGTATATTGAGGGACAGGA
AGATATGGAAGTACTTGGCGTGGCGTATAATGGCCAGGAATGTCTGACATTATTTAAAGATAAAGAGCCCGACGTTCTCC
TCTTAGATATTATCATGCCTCATTTAGACGGTCTTGCTGTACTGGAGCGCCTCCGTGAAAACAATGACATGACCAAACAG
CCAAGTGTGATTATGCTAACGGCGTTCGGTCAAGAAGATGTCACGAAAAAAGCAGTTGATTTGGGCGCATCCTATTTCAT
CTTAAAGCCATTTGATATGGAAAACCTAGTCGGTCATATTCGTCAAGTCAGTGGAAACGGCACACAAGTCACCCATCGAT
CTTCATCTATCCAAAACAGTGTCCTTCGGAACAAACCTGAACCGAAACGTAAAAACTTAGATGCGAGCATTACGACGATT
ATTCATGAGATTGGCGTACCCGCTCATATTAAAGGATATTTATACTTAAGAGAAGCGATTTCGATGGTGTACAATGACAT
TGAGCTTCTCGGAAGCATCACAAAAGTGTTGTACCCAGACATCGCGAAGAAATTTAACACGACTGCCAGCAGGGTAGAAC
GAGCGATTCGCCATGCGATTGAAGTTGCTTGGAGCAGAGGAAACATCGATTCCATTTCTTCACTCTTTGGTTATACAGTC
AGCATGTCTAAAGCGAAACCAACCAATTCTGAATTTATCGCGATGGTAGCTGATCGTCTGCGCTTAGAGCATAGAGCTAG
CTAG

Domains

Predicted by InterproScan.

• Sporulation initiation factor Spo0A C terminal (Spo0A_C) (157-260)

• Response regulator receiver domain (Response_reg) (6-120)

3D structure

• AlphaFold DB: W6IDW7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
spo0A	Bacillus subtilis subsp. subtilis str. 168	88.433	100	0.888