Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   GKC25_RS07480 Genome accession   NZ_CP046128
Coordinates   1509938..1510840 (+) Length   300 a.a.
NCBI ID   WP_095285166.1    Uniprot ID   A0AAE4B8C4
Organism   Bacillus pumilus strain V17C11     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1504938..1515840
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC25_RS07460 (GKC25_07390) - 1505604..1507340 (+) 1737 WP_034662394.1 hypothetical protein -
  GKC25_RS07465 (GKC25_07395) - 1507337..1507612 (+) 276 WP_003211418.1 FlhB-like flagellar biosynthesis protein -
  GKC25_RS07470 (GKC25_07400) sucC 1507789..1508949 (+) 1161 WP_012009945.1 ADP-forming succinate--CoA ligase subunit beta -
  GKC25_RS07475 (GKC25_07405) sucD 1508972..1509874 (+) 903 WP_003212080.1 succinate--CoA ligase subunit alpha -
  GKC25_RS07480 (GKC25_07410) dprA 1509938..1510840 (+) 903 WP_095285166.1 DNA-processing protein DprA Machinery gene
  GKC25_RS07485 (GKC25_07415) topA 1511034..1513109 (+) 2076 WP_060596250.1 type I DNA topoisomerase -
  GKC25_RS07490 (GKC25_07420) trmFO 1513180..1514484 (+) 1305 WP_034662383.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  GKC25_RS07495 (GKC25_07425) xerC 1514547..1515464 (+) 918 WP_342689642.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 300 a.a.        Molecular weight: 34102.37 Da        Isoelectric Point: 8.5955

>NTDB_id=401379 GKC25_RS07480 WP_095285166.1 1509938..1510840(+) (dprA) [Bacillus pumilus strain V17C11]
MYNVSERMIFHRLKGLISPSLLTKWWKVDPELYINEETQHFKQDRSLQTIDFTRLKQAEENEFPIFQHIVQAYLKQNIHM
IPITSPLYPSTLKHIYDPPPVLFLKGNISYLNEEKSLGVVGTRVPSSYGEACVKKIVGELVKEDWTIVSGLAKGIDGLSH
KECIRNKGKTIGIIAGGFQHLYPKEHVQMAQYMGEHHLLLSEHPPYVKPEKWHFPMRNRLISALTSGTIVIQCKEKSGSL
ITAYQALEQGKEVFAVAGSIFDPNSTGPARLIQQGAKLVHSTKDILEEFSFRSVQYTELS

Nucleotide


Download         Length: 903 bp        

>NTDB_id=401379 GKC25_RS07480 WP_095285166.1 1509938..1510840(+) (dprA) [Bacillus pumilus strain V17C11]
ATGTACAACGTGTCCGAAAGAATGATTTTTCACCGCTTAAAAGGCCTCATCTCACCCTCTTTGTTAACAAAATGGTGGAA
AGTCGATCCTGAGCTATATATAAATGAAGAAACACAACATTTCAAACAGGATCGATCATTACAAACGATCGACTTTACCC
GCTTAAAACAAGCCGAAGAAAATGAATTCCCCATTTTTCAACACATCGTTCAAGCCTATTTAAAGCAAAACATTCACATG
ATACCCATCACATCACCCTTATATCCCAGCACACTAAAACATATTTATGATCCTCCCCCTGTGTTATTCCTAAAAGGAAA
CATATCATATTTAAATGAAGAAAAAAGTTTAGGTGTAGTAGGCACACGAGTTCCATCGTCTTATGGAGAAGCATGTGTGA
AGAAAATTGTTGGTGAGCTTGTAAAGGAAGATTGGACGATTGTCAGTGGCTTAGCAAAAGGAATTGATGGACTTTCACAT
AAAGAGTGCATTCGGAATAAAGGGAAAACCATCGGTATTATAGCAGGCGGATTTCAGCACTTATACCCAAAGGAACATGT
GCAAATGGCTCAATACATGGGAGAGCATCATTTGCTTTTATCCGAGCACCCGCCTTATGTGAAACCAGAAAAGTGGCATT
TTCCAATGAGGAATCGTTTAATTAGTGCGCTGACAAGTGGAACCATCGTGATTCAGTGCAAAGAAAAGAGCGGTTCGCTC
ATTACCGCGTATCAAGCACTTGAGCAAGGCAAAGAGGTATTTGCGGTCGCAGGATCTATCTTTGATCCTAATTCCACAGG
ACCAGCCAGACTTATACAGCAGGGAGCAAAGCTTGTTCATTCAACGAAGGATATTTTAGAGGAATTCTCCTTCCGCAGCG
TTCAATATACTGAACTCTCATAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(75-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

55.401

95.667

0.53
  dprA Legionella pneumophila strain ERS1305867

37.705

100

0.383
  dprA Lactococcus lactis subsp. cremoris KW2

38.889

96

0.373

Multiple sequence alignment