Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   GKC25_RS05010 Genome accession   NZ_CP046128
Coordinates   1034916..1038620 (+) Length   1234 a.a.
NCBI ID   WP_187704438.1    Uniprot ID   -
Organism   Bacillus pumilus strain V17C11     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1029916..1043620
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC25_RS04995 (GKC25_04975) - 1030208..1030618 (+) 411 WP_034663477.1 DoxX family protein -
  GKC25_RS05000 (GKC25_04980) - 1030815..1031261 (-) 447 WP_034663474.1 ferritin-like domain-containing protein -
  GKC25_RS05005 (GKC25_04985) addB 1031429..1034938 (+) 3510 WP_034663471.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  GKC25_RS05010 (GKC25_04990) addA 1034916..1038620 (+) 3705 WP_187704438.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  GKC25_RS05015 (GKC25_04995) sbcD 1038647..1039822 (+) 1176 WP_034663467.1 exonuclease subunit SbcD -
  GKC25_RS05020 (GKC25_05000) - 1039819..1043214 (+) 3396 WP_034663465.1 SMC family ATPase -
  GKC25_RS05025 (GKC25_05005) - 1043234..1043533 (+) 300 WP_034663464.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142493.44 Da        Isoelectric Point: 5.0448

>NTDB_id=401366 GKC25_RS05010 WP_187704438.1 1034916..1038620(+) (addA) [Bacillus pumilus strain V17C11]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEHPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPLPEQWMRAFLSLYDVDAQTKVEELPFYPYIKEDLSLVLRSCQELLERA
LSLSKEPGGPAPRAENFIDDLEQVNELIHHQDDFEELYELLPNVNFKRLKTCKGDEYDPVLLEKATDARNQAKKQLEKLK
DEYFMRSPSQHLKSLAEMKPVVETLVELVIKFGERFERAKQEKSIVDFSDLEHYCLRILAEQDAEGHLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEHSAEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKHFQPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESKDTTTEMLLVHLDQQEAESDEEREELETVQFEARIIAKK
IRELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPIVHLDENEMALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFQLLHKWRDFSINHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEEWLLPDFERYQSKTYLDFIGPALIRHQAMSSILEETGDVVLSHPSTFTISFTQASDL
LKEDMSLEKKQQDEVVQALMDGLPVEGYGDADEKVAERLSWKYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSIRKAR
ATLYDRPAFMKKKTLTAAEQGTAMHTVMQHITLPSEEPYDESRIRHLLESLQQRDLLTDEQVRSIDQEGIAAFFSTSIGQ
KLLKADWVKREVSFSMVLPVKEVYSHIDTEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYTGGLDAAESILKKRYE
TQIALYAKAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=401366 GKC25_RS05010 WP_187704438.1 1034916..1038620(+) (addA) [Bacillus pumilus strain V17C11]
ATGCAAATACCAAAACCGAATAACAGTACGTGGACGGATGACCAATGGGAAGCCATCGTTTCAGAAGGACAAGATATCTT
GGTTGCGGCGGCGGCAGGGTCAGGAAAAACAGCTGTTTTGGTTGAACGGCTGATTCGAAAAATGACCCGGCCTGAACATC
CAGTTGATGTGGACCGTCTGCTCGTTGTGACTTTCACAAATGCATCTGCGGCAGAGATGAAGCATCGGATTACAGAAGCA
CTTGAAAAAGAATTAGCCAAAAACCCTGGGTCCCTTCATATGAGAAGGCAGCTGTCCCTCATGAATCGTGCTAATATTTC
TACCTTGCACTCCTTTTGCTTGCAGGTTTTGCGCACCTTTTACTATGAAATTGATCTTGACCCAGGCTTTCGTTTGGCAG
ATCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGATGAACTGTTCGAGGATGAATATAAGGCTGGAAAGCCATCA
TTCTTTGAACTGGTTGATCGTTACACTTCTGACCGCCATGACTTAGATTTGCAATGGCTTGTGAAGAGAATTTATGAGTT
TTCTAGGTCTCATCCTTTACCTGAGCAATGGATGAGGGCATTCCTTTCACTTTATGATGTAGATGCACAAACAAAAGTAG
AAGAATTACCGTTTTATCCATATATCAAAGAAGATCTTTCTCTCGTTCTCCGGAGCTGCCAGGAGCTGCTTGAACGAGCT
CTTTCGCTATCAAAAGAGCCGGGCGGCCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
AATCCATCATCAAGATGATTTTGAGGAGCTTTACGAACTTTTGCCGAACGTCAATTTTAAAAGGCTCAAAACGTGCAAAG
GGGATGAATATGACCCTGTTTTGTTAGAAAAGGCCACAGATGCACGTAATCAAGCAAAAAAACAACTAGAAAAGCTAAAA
GATGAATACTTTATGCGCAGTCCTTCCCAGCATTTAAAAAGCTTAGCTGAAATGAAGCCAGTTGTGGAGACGCTTGTAGA
GCTTGTGATCAAATTTGGTGAGCGTTTCGAAAGAGCGAAGCAGGAAAAGTCAATCGTCGACTTTTCGGATTTAGAGCACT
ATTGCTTACGCATTTTAGCGGAGCAGGATGCAGAAGGACATTTGATCGAAACAGAAGCTGCTAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTTGTTGATGAATACCAGGATACAAACCTCGTACAAGAAACGATTTTAAAGCTTGTGTCTAAAGGGGA
ACATTCTGCAGAGGGCAATCTGTTTATGGTTGGTGATGTAAAGCAGTCCATTTATCGTTTTAGACTGGCTGAGCCCATGC
TCTTTTTAAACAAATATAAACACTTTCAACCAGACGGCAAAGAAACAGGGAAGAGAATTGATTTAAATAAAAACTTCCGA
AGCCGTTCAGATGTTTTAGATAGTACGAACTTTTTGTTCAAGCAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGCAAAGATACAACGACAGAAATGCTCCTTGTCCACTTAG
ATCAGCAGGAAGCGGAAAGCGACGAAGAGCGAGAAGAACTTGAGACCGTCCAATTTGAAGCGAGAATCATTGCTAAAAAA
ATAAGAGAATTGGTGGAGCAGCCATTTCAAGTGTACGATGCAAAACAGCAAATGACCCGTAACTTGCAGTATCGTGACAT
TGTCATTTTGCTTCGGTCTATGCCGTGGGCTCCGCAAATGATGGAAGAATTAAAGAAGCAGGGAATCCCTGTATATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTCATTCTTTCTTTGTTAAAAGTCATCGATAATCCGTATCAG
GATATTCCGCTTGCAGCTGTTTTAAGATCACCTATTGTTCATTTAGATGAAAATGAAATGGCACTTATTCGAACGAGTGA
TAAAAAAGGCACTTATTATGATGCTGTAAAGGCATTCATGAGTGTGACGCATTCAGATCACCCTACATGCAAAAAATTAG
AGCGATTTTTCCAATTGCTGCATAAGTGGCGTGATTTCTCCATCAATCATTCGGTGGCAGAATTAATTTGGGAAGTCTAC
CGAGATACGCAGTATCTTGATTATGTTGGCGGGATGCCTGGAGGTAAGCAGCGGCAGGCGAATCTTCGTGCCCTTTATGA
TCGAGCCAAGCAATATGAAAAAGCTGCCTTTAGAGGGCTATTTCGATTCCTTCGTTTTATTGAAAGAATGCAGGAGCGTG
GAGATGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAGGACGTTGTGCGCATGATGACGATCCACAGCAGTAAAGGA
TTAGAATTTCCAGTCGTCTTTACTGCAGGACTCGGCAGGAACTTTAATATGATGGACTTAAACCAGTCCTACTTACTTGA
TAAAGAGCTAGGCTTTGGAAGCAAGTATATCCATCCTGAGCTGAGAATTAGTTATGCGACATTACCGCTTGTGGCGATGA
AAAAAAAGATGAGAAAAGAGTTATTATCTGAAGAGCTGAGGGTCCTATATGTGGCGCTCACAAGGGCTAAGGAAAAATTA
TTTCTGGTCGGCTCTGTCAAAAATCAGGTGAAAGCATTAAGCAAATGGCAAAATGCCGCGACTGGAGAAGAGTGGCTGCT
CCCTGATTTTGAACGTTATCAATCGAAAACATACTTAGATTTTATTGGTCCTGCTCTCATCCGTCATCAAGCGATGTCAT
CTATTTTAGAAGAAACAGGCGATGTCGTTCTTTCGCATCCGTCCACATTCACCATCTCATTTACTCAAGCTTCTGATCTT
CTAAAGGAAGATATGTCACTTGAAAAGAAGCAGCAAGACGAGGTAGTACAAGCATTGATGGATGGTCTGCCTGTTGAAGG
ATATGGTGATGCTGATGAGAAGGTGGCAGAGAGACTTTCATGGAAATATCCATATCTAGCGGCCTCGCAAGTCGGCACAA
AGCAGTCTGTTTCCGAGATAAAAAGGATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTATTCGAAAAGCTCGT
GCAACATTGTATGACCGGCCGGCTTTCATGAAGAAAAAAACACTCACTGCCGCAGAACAAGGCACCGCAATGCATACAGT
CATGCAGCATATCACGCTTCCTTCTGAGGAGCCCTATGATGAATCACGTATAAGGCACCTGCTCGAATCCTTACAGCAGC
GAGACTTGCTAACAGATGAGCAAGTCCGGTCAATTGATCAAGAAGGAATTGCTGCTTTCTTCTCTACTTCAATTGGTCAA
AAACTGCTGAAAGCGGACTGGGTAAAACGAGAAGTATCATTTAGTATGGTGTTACCTGTTAAAGAGGTATACAGTCATAT
TGATACTGAGGGAGAGCCTGTTCTGATTCAAGGAATGATTGACTGTTTATTTGAAACAGATGGAAAGCTATATTTACTAG
ATTATAAGACGGATAGAGTGCAAGGCAGATATACAGGTGGACTTGACGCTGCTGAATCTATCTTGAAAAAGCGCTATGAA
ACCCAAATAGCGTTATATGCAAAAGCAGTAGAGCGTTTGACAAACAGAACATTAGAGGAGAAAATTCTCTATTTCTTCGA
TGGGAATTTAGAAATCTCTTTATAA

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-463)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1226)

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.343

100

0.665

Multiple sequence alignment