Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   GKC25_RS05005 Genome accession   NZ_CP046128
Coordinates   1031429..1034938 (+) Length   1169 a.a.
NCBI ID   WP_034663471.1    Uniprot ID   -
Organism   Bacillus pumilus strain V17C11     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1026429..1039938
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC25_RS04975 (GKC25_04955) lepB 1026938..1027450 (+) 513 WP_034663490.1 signal peptidase I -
  GKC25_RS04980 (GKC25_04960) - 1027485..1028630 (-) 1146 WP_034663485.1 AbrB family transcriptional regulator -
  GKC25_RS04985 (GKC25_04965) - 1028897..1029319 (+) 423 WP_187704437.1 BlaI/MecI/CopY family transcriptional regulator -
  GKC25_RS04990 (GKC25_04970) - 1029312..1030151 (+) 840 WP_034663481.1 M56 family metallopeptidase -
  GKC25_RS04995 (GKC25_04975) - 1030208..1030618 (+) 411 WP_034663477.1 DoxX family protein -
  GKC25_RS05000 (GKC25_04980) - 1030815..1031261 (-) 447 WP_034663474.1 ferritin-like domain-containing protein -
  GKC25_RS05005 (GKC25_04985) addB 1031429..1034938 (+) 3510 WP_034663471.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  GKC25_RS05010 (GKC25_04990) addA 1034916..1038620 (+) 3705 WP_187704438.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  GKC25_RS05015 (GKC25_04995) sbcD 1038647..1039822 (+) 1176 WP_034663467.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134748.77 Da        Isoelectric Point: 5.1968

>NTDB_id=401365 GKC25_RS05005 WP_034663471.1 1031429..1034938(+) (addB) [Bacillus pumilus strain V17C11]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEQKEKFKVFKKASDKPGFVEQIEKTMAEFKRYCMLPEDIEKISMSSMYSEYTEERRAAE
KLHDLHVLYQQMEQYLQDEYVHSEDYLTLLTQQIPSSEEIKGAHIYIDGFYQFTPQQLLVIEQLLLHAAKITAAFTVDRS
YHDKQPNELDLFRMTGKTYFQLYQLARECGADISENVLERNKRHIYTPDLAYLEHQYEQRPVQPYQENTPHLTVSKSANK
RAEIEGVARDILDLVREKGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLELAKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IVPPLYQLQIRLKKAQKVRDMVEAVYVFLEEIQVPDKLEKARLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVEMLGEEEL
SFPLFQQMMDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCTFIVGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSRHLYLSYSAADAEGRSLLPSTLIKYCQELMPNHQQALYVLDPEQLDDEEQLKFVANEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNYLMTSPNRDVSKNIMSSLFFTNRAKPLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKEDCVTYSKHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGVEATPAGILYFHIHDPLIQAPIPLAEDDIEQEIFKKFKMKGL
LLEDIEAVKLMDQTLDSGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTQHVRRTFEEAGERITNGEVAINPYKLKDQTP
CRFCSFKSICQFDESIEDNDFRVLTSEKDDVVIDRIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=401365 GKC25_RS05005 WP_034663471.1 1031429..1034938(+) (addB) [Bacillus pumilus strain V17C11]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCTTGATCCGTTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTTTTAATGGAATATGAGCTTGCAAAAA
CGTCTGAAGCTGGCGGAATGATCAGGGCCAAAGTATTTAGTTTCACACGTCTTGCTTGGTCTATTTTACAGCAGACTGGC
GGAGCGAACCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTGATTGAAGAGCAGAAAGAAAAGTT
TAAAGTATTCAAAAAAGCGAGTGATAAGCCGGGATTTGTGGAGCAAATTGAAAAGACGATGGCTGAATTCAAAAGGTATT
GTATGTTGCCGGAGGATATTGAAAAAATTTCAATGTCGAGCATGTATTCAGAGTATACAGAAGAAAGACGGGCAGCCGAA
AAATTGCATGATCTGCATGTTCTTTATCAGCAGATGGAGCAGTATTTACAAGATGAATATGTGCACTCAGAAGACTATTT
GACACTTCTTACGCAGCAAATTCCTTCATCTGAAGAAATAAAAGGAGCACACATTTATATAGATGGTTTTTATCAATTTA
CACCGCAGCAGCTTCTTGTCATTGAACAGCTCCTGCTGCATGCAGCAAAAATAACTGCTGCTTTCACAGTGGATCGCTCC
TATCATGATAAGCAGCCAAATGAACTTGATTTATTTCGTATGACAGGTAAAACGTATTTCCAGCTCTATCAGCTTGCTAG
AGAGTGCGGAGCTGACATTTCTGAAAACGTTTTGGAAAGAAATAAACGGCACATTTATACACCAGATCTTGCTTATTTAG
AACACCAATATGAGCAGAGACCAGTGCAGCCATATCAGGAAAACACCCCTCATCTCACAGTGTCTAAAAGTGCAAATAAA
CGAGCTGAAATTGAGGGAGTAGCCAGAGATATACTTGATTTAGTGAGAGAAAAAGGACTTAGATTGAGAGATATCTCGAT
TGTTGCACGACATGTAGACGACTATAAAGATACATTAAAAGAGGTTTTTCGAGATTACGATATTCCTTTTTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATTGAATTGATTCGTTCGAGCTTGGATGTCATAAAGGGAAACTGGCGGTAT
GAAGCGGTATTTCGCTGCGTGAAAACAGAGTTCTTATTTCCGCTTGAACTCGCTAAGAACAAAGCGAGAGAGCAGGCAGA
TCAGCTCGAAAACTATTGTATTGCTTATGGTGTAAAAGGAGAACGCTGGACAAACGGTTCCCGGTTTCATTACAGGCGCT
TCCAATCATTAGATGAGGATTTTGGACAAACAGATCAAGAAATTGAAATGGAACAAATGCTAAATGACGTCAAAGAATGG
ATCGTGCCCCCGCTTTACCAGTTGCAGATTAGGCTCAAAAAAGCGCAAAAGGTAAGAGACATGGTAGAGGCTGTCTATGT
CTTTTTAGAAGAGATACAAGTACCAGATAAGCTTGAAAAAGCGAGGCTTGAGGCTGAAGAAGCAGGTAGATTAGCTGAAG
CAATGCAGCACGGACAAGTATGGGATGCGGTTATTCAATTAATGGATGAATTTGTTGAAATGCTAGGTGAAGAAGAGCTC
TCATTTCCTTTATTCCAACAAATGATGGATACAGGATTAGCTTCTCTAAAATTCGCTTTGATTCCGCCTTCACTCGACCA
AGTATTTATTGGAAGTATGGATTTATCGAGAATGTATCAAGTGAAATGTACGTTTATCGTTGGTGTAAATGATGGCGTTA
TTCCCGCACGCCCCTCTGATGAGAGTGTATTGTCTGAGGATGACCGTGAATGGTTAAAACGAGCAGGAGCAGAGCTGGCA
GAGACAGGAAAGGAACGGCTGCTAGATGAGCAATTTTTAATTTACCAAGCGTTATCGAGTCCATCACGTCATTTGTATCT
ATCCTATTCGGCTGCTGATGCAGAAGGACGTTCTCTTTTGCCTTCGACACTCATCAAGTATTGTCAAGAGCTTATGCCAA
ATCATCAGCAGGCTCTTTATGTGTTAGATCCAGAACAACTGGATGACGAGGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTTTCGTTATCGTATACCGTCTCACAGCTGCAGCAATGGCTCAATCAATATCCCATTAGTGGTGTATGGTGGAGTGTCTA
TAACTACTTAATGACATCTCCTAATCGGGATGTATCAAAAAACATCATGTCAAGTTTGTTTTTTACAAATCGAGCGAAGC
CATTAAAGCCTAATGTCACAAAAGAGCTTTATGGTGATCATATTCAGGGCAGTGTTTCAAGAATGGAGAAATTCAATGCG
TGTGCATTTTCTCACTTTGCTTCCCATGGGTTAAAACTAAAGGATCGACAATTTTACAAATTGGAAGCACCTGATATCGG
CCAGTTGTTTCACTCGGCTTTAAAACATATTTCAGATACGCTTGTTGAACAAAAAAAGGATTGGAAAAACCTAACGAAGG
AAGATTGTGTCACTTATTCAAAACATGCGATTGAACAGCTTGCTCCGCGTCTTCAAAAGGAAATTCTATTAAGCTCTAAT
CGGCATGCTTATATTAAAGAAAAGCTCCAGAAAATCTTGATTCGAGTCTCTTCTATATTAAGTGAACATGCCAAAGTGAG
TGGATTTTCTCCAGTAGGGCTAGAGTTAGGTTTTGGGGGGCAAGGGCCATTGCCGCCGTTTACTTTTCAATTAAAGAATG
GCTGCACGATGGAGCTTGTCGGAAGAATTGACCGGGTAGATAAAGCAGAAGGTTCAAAAGGGCTGCTCTTGAGAATAGTC
GACTACAAATCCAGTGAGAAAGGTCTTGACTTAGCAGAAGTATACTATGGGCTGGCCTTGCAAATGCTCACTTACTTAGA
CCTGACCATTACGTATTCGAAAGAGTGGCTCGGGGTTGAAGCGACGCCTGCTGGCATTTTATATTTCCATATCCATGATC
CGCTCATTCAAGCACCTATTCCGCTTGCAGAGGATGACATTGAGCAGGAAATATTCAAGAAATTTAAAATGAAGGGTTTA
TTGCTTGAAGATATAGAAGCAGTAAAGCTCATGGATCAAACGCTTGATTCAGGTAGATCACAAGTCATTCAAGCCGGCTT
GAAAAAGGATGGGTCTTTCCGTTCTGACTCTGCAGTTTTAAGTGAAGATCATTTCCATATACTCACACAACACGTTCGGC
GCACATTTGAAGAAGCAGGCGAAAGAATTACAAATGGAGAGGTCGCAATTAATCCATATAAATTGAAGGATCAAACACCT
TGCCGCTTTTGTTCATTCAAGTCTATTTGCCAATTCGATGAATCAATAGAGGATAATGATTTTAGGGTGCTCACCTCTGA
GAAAGATGATGTTGTGATAGACCGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

66.15

99.316

0.657

Multiple sequence alignment