Detailed information NTG0401164

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GJ746_RS04670
• Genome accession: NZ_CP046115
• Coordinates: 998306..999106 (-)
• Length: 266 a.a.
• NCBI ID: WP_154679144.1
• Uniprot ID: A0A6B8MSP6
• Organism: Klebsiella oxytoca strain P620
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 993306..1004106

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GJ746_RS04650 (GJ746_04650)	dksA	993835..994290 (-)	456	WP_004098689.1	RNA polymerase-binding protein DksA	-
GJ746_RS04655 (GJ746_04655)	sfsA	994455..995171 (-)	717	WP_154679141.1	DNA/RNA nuclease SfsA	-
GJ746_RS04660 (GJ746_04660)	thpR	995171..995707 (-)	537	WP_154679142.1	RNA 2',3'-cyclic phosphodiesterase	-
GJ746_RS04665 (GJ746_04665)	hrpB	995780..998209 (+)	2430	WP_154679143.1	ATP-dependent helicase HrpB	-
GJ746_RS04670 (GJ746_04670)	pilD	998306..999106 (-)	801	WP_154679144.1	prepilin peptidase	Machinery gene
GJ746_RS04675 (GJ746_04675)	-	999106..999864 (-)	759	WP_154679145.1	type II secretion system protein N	-
GJ746_RS04680 (GJ746_04680)	-	999854..1000339 (-)	486	WP_154679146.1	type II secretion system protein M	-
GJ746_RS04685 (GJ746_04685)	gspL	1000332..1001528 (-)	1197	WP_154679147.1	type II secretion system protein GspL	-
GJ746_RS04690 (GJ746_04690)	gspK	1001521..1002501 (-)	981	WP_154679148.1	type II secretion system minor pseudopilin GspK	-
GJ746_RS04695 (GJ746_04695)	gspJ	1002498..1003094 (-)	597	WP_154679149.1	type II secretion system minor pseudopilin GspJ	-
GJ746_RS04700 (GJ746_04700)	gspI	1003091..1003456 (-)	366	WP_154679150.1	type II secretion system minor pseudopilin GspI	-
GJ746_RS04705 (GJ746_04705)	gspH	1003453..1003965 (-)	513	WP_154679151.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29398.19 Da
• Isoelectric Point: 6.6870

>NTDB_id=401164 GJ746_RS04670 WP_154679144.1 998306..999106(-) (pilD) [Klebsiella oxytoca strain P620]
MIENIALLPEFAAQYPLIWSGFLLLFGLAFGSFFNVVIHRLPLMMEQTEAMNLCFPASFCPQCHQPIAWRDNIPLLSFLW
LKGRSRCCGLPIPHRYPLIELASGALFMLIGYLFPPGIPLLGGLLLLSVLLILAAIDAQTQLLPDRLTLPLLWAGLLFNL
SDTFVPLAESVIGAMAGYLSLWSVYWIFRLLTATEALGYGDFKLLAALGAWLGWQALPQTLLLASASGLMWTLLQRLVTR
RSLEQPLAFGPWLALAGGGIFLWGQV

Nucleotide

• Length: 801 bp

>NTDB_id=401164 GJ746_RS04670 WP_154679144.1 998306..999106(-) (pilD) [Klebsiella oxytoca strain P620]
ATGATAGAAAACATCGCGCTGTTGCCGGAGTTCGCCGCGCAATATCCGCTTATCTGGAGCGGATTTTTACTCCTTTTTGG
CCTGGCGTTCGGCAGCTTTTTTAACGTCGTTATCCACCGTTTACCGCTGATGATGGAACAAACGGAGGCAATGAATCTCT
GTTTTCCCGCTTCGTTCTGTCCGCAGTGCCACCAGCCTATCGCCTGGCGGGACAATATTCCGCTGCTGAGCTTCCTGTGG
CTGAAGGGGCGTTCACGCTGTTGTGGGCTACCGATTCCCCATCGTTATCCGCTTATCGAGCTGGCGAGCGGCGCGCTGTT
TATGCTGATCGGATACCTGTTCCCACCGGGCATACCGCTGCTGGGCGGCTTACTTCTGCTGTCGGTTCTACTGATTCTTG
CAGCCATCGACGCGCAAACGCAGCTGCTGCCCGATCGGCTGACACTCCCGCTGCTGTGGGCCGGGCTGCTGTTCAACCTT
AGCGATACCTTTGTACCGCTGGCAGAATCGGTCATCGGCGCAATGGCCGGGTATTTGTCGCTGTGGTCGGTGTACTGGAT
ATTTCGTCTGCTGACAGCTACAGAGGCGCTGGGGTACGGCGATTTTAAACTGCTGGCGGCGCTCGGCGCGTGGCTCGGCT
GGCAGGCGCTGCCGCAGACGCTGCTGCTGGCTTCCGCCAGCGGCCTGATGTGGACGCTGCTGCAGCGGCTGGTAACCCGT
CGTTCGCTCGAACAACCTCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCGGCGGGATATTTTTGTGGGGCCAGGTTTA
A

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (25-112)

• Type IV leader peptidase family (Peptidase_A24) (124-232)

3D structure

• AlphaFold DB: A0A6B8MSP6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	47.407	100	0.481
pilD	Vibrio campbellii strain DS40M4	44.727	100	0.462
pilD	Acinetobacter nosocomialis M2	45.174	97.368	0.44
pilD	Acinetobacter baumannii D1279779	44.402	97.368	0.432
pilD	Neisseria gonorrhoeae MS11	44.706	95.865	0.429