Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   D3800_RS16060 Genome accession   NZ_CP046058
Coordinates   3436365..3438380 (-) Length   671 a.a.
NCBI ID   WP_002778011.1    Uniprot ID   A0A2H6BPT5
Organism   Microcystis aeruginosa NIES-298     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3431365..3443380
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D3800_RS16050 (D3800_16050) - 3433757..3434971 (-) 1215 WP_103111795.1 type II secretion system F family protein -
  D3800_RS16055 (D3800_16055) pilT 3435113..3436219 (-) 1107 WP_103111796.1 type IV pilus twitching motility protein PilT Machinery gene
  D3800_RS16060 (D3800_16060) pilB/pilB1 3436365..3438380 (-) 2016 WP_002778011.1 GspE/PulE family protein Machinery gene
  D3800_RS16065 (D3800_16065) grpE 3438621..3439343 (+) 723 WP_002738986.1 nucleotide exchange factor GrpE -
  D3800_RS16070 (D3800_16070) - 3439438..3440079 (-) 642 WP_103111797.1 HAD-IA family hydrolase -
  D3800_RS16075 (D3800_16075) - 3440170..3442134 (-) 1965 WP_103111798.1 amylo-alpha-1,6-glucosidase -
  D3800_RS16080 (D3800_16080) rpmH 3442225..3442362 (+) 138 WP_002739276.1 50S ribosomal protein L34 -
  D3800_RS16085 (D3800_16085) rnpA 3442389..3442751 (+) 363 WP_002738574.1 ribonuclease P protein component -
  D3800_RS16090 (D3800_16090) - 3442741..3443136 (+) 396 WP_002738951.1 PH domain-containing protein -

Sequence

Protein


Download         Length: 671 a.a.        Molecular weight: 75086.22 Da        Isoelectric Point: 6.0473

>NTDB_id=400763 D3800_RS16060 WP_002778011.1 3436365..3438380(-) (pilB/pilB1) [Microcystis aeruginosa NIES-298]
MTFSSKKTRAVALRNYFSPFGNKLVQSGYIGAEQMQQALVETRKSGRSLVEILQKLTGRPLPPDLQRQYKKNQLFELKIL
YGVESLDPELSDVDGLEIARLIDSLIPIDLCRRYRLIPLRRIEGEPAAILIAMVDPDNLEATDDINRILRPRDLGLQRLV
IAGEDYERLLEKFHSAQSELEREKARLQKEKELEKLSDLTDIVGSLDLTSGVANDEVADDLGEGDANQAPVINLVNKIIA
KALQEGSSDIHIEPQEEFLKIRFRKDGVLHQAFEPLPKKIAPAVTARFKIMADLDISERRLPQDGKIRRMYQGRKVDFRV
NTLPSRYGEKVCLRILDNSATQLGLDKLITDRNTLQIVRDMASRPFGLLLVTGPTGSGKSTTLYSVLAERNHPGVNISTA
EDPIEYSLPGITQVQIIREKGMDFASILRAFLRQDPDVILVGETRDRETAKTVIEAALTGHLVLTTLHTNDAAGAIVRLD
KMGIEPFMISGSLLGILAQRLVRRVCTQCRVAYHPSQEELARFGLSAANEQEVTFYKANTLTMEEIQQARKQGNLCLKCA
GSGYKDRIGVYEVMQNSERIQQLINQGATTDSIKEAAVDEGMVTLLAYSLNLVREGYTTLEEVERVTLTDSGLEAELKAK
RKSGLICSGCRAELQPEWLDCPYCMTPRFSY

Nucleotide


Download         Length: 2016 bp        

>NTDB_id=400763 D3800_RS16060 WP_002778011.1 3436365..3438380(-) (pilB/pilB1) [Microcystis aeruginosa NIES-298]
ATGACATTTTCTTCCAAGAAAACCCGTGCTGTCGCCCTACGCAATTACTTTTCCCCCTTTGGTAACAAGTTAGTGCAGTC
GGGCTATATCGGTGCCGAGCAAATGCAACAGGCCCTGGTAGAAACGCGGAAATCGGGACGATCGCTAGTAGAAATATTGC
AAAAACTAACCGGGCGGCCTTTGCCCCCGGATTTACAGCGACAGTATAAGAAAAACCAGTTATTTGAACTAAAAATCCTC
TACGGAGTCGAATCGCTCGACCCAGAATTGAGCGATGTGGATGGACTGGAAATAGCCAGGCTGATCGATTCTTTGATTCC
GATCGATCTTTGTCGTCGCTATCGTCTCATCCCCCTGCGTCGAATCGAAGGAGAGCCAGCCGCGATTTTGATAGCCATGG
TCGATCCTGACAACCTCGAGGCTACTGACGATATCAATCGTATCCTCCGCCCCAGGGATTTAGGTTTACAGCGTTTGGTG
ATTGCCGGGGAAGATTATGAAAGATTACTGGAAAAATTCCACTCGGCTCAATCGGAACTAGAACGGGAGAAAGCCCGACT
GCAAAAAGAAAAAGAACTGGAAAAACTCTCGGATCTGACCGATATTGTCGGCAGTCTTGATTTGACCTCTGGTGTTGCTA
ACGATGAAGTGGCCGATGATCTCGGAGAAGGGGATGCTAATCAAGCCCCCGTCATCAACCTTGTCAATAAAATTATAGCT
AAAGCTCTACAGGAAGGATCCTCCGATATTCACATCGAACCCCAAGAAGAATTCCTGAAAATTCGCTTTCGCAAAGATGG
AGTTCTTCATCAAGCTTTTGAGCCACTGCCGAAAAAAATTGCCCCGGCGGTCACAGCCCGGTTTAAAATCATGGCCGATC
TCGATATTTCCGAACGTCGTCTTCCCCAGGATGGTAAAATTCGGCGGATGTATCAAGGACGAAAAGTAGATTTTCGGGTT
AATACCCTGCCCAGTCGCTACGGGGAAAAAGTCTGTTTAAGGATTCTCGATAACTCGGCTACCCAGTTGGGTTTAGATAA
ACTAATCACTGATCGAAATACTCTGCAAATCGTCCGAGACATGGCTAGTCGTCCCTTTGGTCTGTTGCTGGTGACAGGCC
CCACCGGTTCAGGGAAATCCACCACTTTATACTCGGTGTTAGCGGAAAGAAATCACCCTGGGGTCAATATTAGTACGGCC
GAAGACCCGATCGAATATTCTCTGCCAGGCATCACCCAGGTGCAGATAATTCGCGAAAAAGGCATGGATTTCGCCTCGAT
TCTGCGGGCATTCCTGCGACAAGACCCGGATGTGATTCTGGTGGGAGAAACTAGGGATAGGGAAACGGCTAAAACGGTGA
TCGAGGCCGCCTTAACCGGTCACTTGGTCTTAACTACCCTACACACCAACGATGCCGCAGGTGCGATCGTTCGTCTTGAT
AAGATGGGGATTGAACCGTTCATGATTTCTGGTTCTCTGTTGGGAATCTTGGCCCAGCGTCTCGTGCGGCGCGTCTGTAC
CCAGTGTCGGGTCGCCTATCACCCTAGTCAAGAGGAATTAGCCCGTTTTGGTCTTTCCGCTGCCAATGAACAGGAAGTGA
CTTTTTATAAGGCTAATACCCTAACTATGGAGGAAATTCAACAAGCGCGAAAACAGGGCAATCTCTGCCTTAAGTGTGCT
GGCAGCGGTTACAAAGATCGCATCGGTGTCTATGAGGTAATGCAGAATAGTGAACGAATACAGCAGTTAATTAACCAAGG
GGCGACAACCGATAGCATTAAGGAGGCGGCCGTTGATGAGGGCATGGTGACACTCCTAGCCTATAGTTTAAATCTGGTGC
GCGAGGGTTACACCACCCTCGAAGAAGTAGAACGGGTGACACTGACCGATTCTGGTCTAGAAGCGGAATTAAAAGCCAAA
CGGAAAAGCGGTTTAATCTGTAGTGGTTGTCGCGCCGAACTACAACCAGAATGGCTCGATTGTCCCTATTGTATGACACC
GCGATTTAGTTATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(237-504)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2H6BPT5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

71.903

98.659

0.709

Multiple sequence alignment