Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilW   Type   Machinery gene
Locus tag   GJD93_RS02640 Genome accession   NZ_CP046045
Coordinates   332061..332990 (+) Length   309 a.a.
NCBI ID   WP_228274490.1    Uniprot ID   -
Organism   Acinetobacter towneri strain 19110F47     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 318521..338588 332061..332990 within 0


Gene organization within MGE regions


Location: 318521..338588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJD93_RS02555 (GJD93_02555) - 318521..319402 (+) 882 WP_154320257.1 IS982 family transposase -
  GJD93_RS02560 (GJD93_02560) - 319392..319556 (-) 165 WP_154320258.1 hypothetical protein -
  GJD93_RS02565 (GJD93_02565) - 319553..320599 (-) 1047 WP_154320259.1 AAA family ATPase -
  GJD93_RS14735 (GJD93_02570) - 321215..321562 (+) 348 WP_154320260.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GJD93_RS14740 (GJD93_02575) - 321627..321788 (+) 162 Protein_307 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GJD93_RS02580 (GJD93_02580) - 321850..322782 (+) 933 WP_154320262.1 IS5-like element ISAba12 family transposase -
  GJD93_RS14560 - 322805..323116 (+) 312 WP_154320263.1 hypothetical protein -
  GJD93_RS02590 (GJD93_02585) - 323210..324844 (+) 1635 WP_154320264.1 PglL family O-oligosaccharyltransferase -
  GJD93_RS02595 (GJD93_02590) bfr 324932..325396 (-) 465 WP_154320265.1 bacterioferritin -
  GJD93_RS02600 (GJD93_02595) - 325644..325838 (-) 195 WP_154320266.1 bacterioferritin-associated ferredoxin -
  GJD93_RS02605 (GJD93_02600) - 326011..326394 (-) 384 WP_004809961.1 RidA family protein -
  GJD93_RS02610 (GJD93_02605) - 326517..328619 (-) 2103 WP_154320267.1 RelA/SpoT family protein -
  GJD93_RS02615 (GJD93_02610) rpoZ 328842..329123 (-) 282 WP_004809957.1 DNA-directed RNA polymerase subunit omega -
  GJD93_RS02620 (GJD93_02615) gmk 329200..329823 (-) 624 WP_131260628.1 guanylate kinase -
  GJD93_RS02625 (GJD93_02620) ispH 329958..330908 (+) 951 WP_154320268.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  GJD93_RS02630 (GJD93_02625) - 331073..331555 (+) 483 WP_154321236.1 GspH/FimT family pseudopilin -
  GJD93_RS02635 (GJD93_02630) pilV 331555..332061 (+) 507 WP_154320269.1 type IV pilus modification protein PilV Machinery gene
  GJD93_RS02640 (GJD93_02635) pilW 332061..332990 (+) 930 WP_228274490.1 PilW family protein Machinery gene
  GJD93_RS02645 (GJD93_02640) - 332987..333796 (+) 810 WP_154320271.1 hypothetical protein -
  GJD93_RS02650 (GJD93_02645) pilY1 333813..337661 (+) 3849 WP_410169794.1 pilus assembly protein PilY Machinery gene
  GJD93_RS02655 (GJD93_02650) - 337648..338160 (+) 513 WP_154320272.1 type IV pilin protein -
  GJD93_RS02660 (GJD93_02655) - 338154..338588 (+) 435 WP_154320273.1 type IV pilin protein -

Sequence

Protein


Download         Length: 309 a.a.        Molecular weight: 33331.47 Da        Isoelectric Point: 6.4953

>NTDB_id=400626 GJD93_RS02640 WP_228274490.1 332061..332990(+) (pilW) [Acinetobacter towneri strain 19110F47]
MNKKPHHLKYSQKGFTLIELIVALALGLLITAAATQLFLGGVVSTRIQQANAELQDSGVFGLEYVARDVRLANYGNISNP
ELTNTTPMGGIVFSQGGANSNVSEDIGETSVTRQGLGSNASENSDQLTIQFIAPNRMFNCEGEEVQAGEYVIQRYFLRKD
NNGAATDLALACDANKHGVTSAAGIANFGGNNEGQVIMPRVDHLRFYLGTMTKDSTHTNITYAYYSINDYIAAVNAARSN
GEPLPRVVSVKAVAVVRSMDNTNNELIDETKPVQGLDRAVTLTAANQTKHLRRTYITTIAIRNGMGDAL

Nucleotide


Download         Length: 930 bp        

>NTDB_id=400626 GJD93_RS02640 WP_228274490.1 332061..332990(+) (pilW) [Acinetobacter towneri strain 19110F47]
ATGAACAAGAAGCCTCATCATCTTAAATATAGTCAAAAGGGTTTTACTTTAATTGAACTCATAGTTGCACTTGCACTTGG
TTTGTTAATTACAGCAGCTGCAACTCAATTGTTTCTAGGCGGTGTTGTCTCAACGCGTATTCAACAAGCTAATGCTGAAC
TTCAGGATAGTGGCGTATTTGGACTAGAGTATGTGGCGAGAGATGTGCGCTTGGCTAATTATGGAAATATTAGTAACCCT
GAGTTGACGAATACAACCCCTATGGGAGGGATTGTATTTTCACAAGGTGGAGCAAATTCCAATGTATCTGAAGATATTGG
GGAAACATCGGTTACTCGGCAGGGACTAGGGTCGAACGCTTCTGAAAACAGTGATCAATTAACGATTCAGTTTATCGCAC
CAAACAGAATGTTCAATTGCGAAGGTGAAGAGGTTCAAGCGGGTGAATATGTGATCCAACGTTATTTCTTGCGAAAAGAT
AATAATGGTGCAGCTACGGATTTAGCACTTGCTTGCGATGCAAATAAGCATGGTGTTACCTCTGCCGCAGGTATTGCAAA
CTTTGGTGGAAATAATGAAGGGCAGGTGATCATGCCTAGAGTTGATCACCTTCGTTTTTATTTGGGCACCATGACTAAAG
ATAGTACGCATACCAATATCACCTATGCATATTATAGCATTAATGACTATATTGCTGCCGTCAATGCTGCACGTAGTAAT
GGAGAACCTCTTCCTAGAGTTGTATCAGTAAAAGCTGTAGCTGTCGTAAGATCTATGGATAACACTAATAATGAATTAAT
AGATGAAACTAAGCCAGTTCAGGGGCTTGATCGTGCAGTAACTCTTACTGCGGCAAATCAAACAAAGCATTTAAGACGAA
CTTATATCACAACCATTGCAATACGTAATGGAATGGGAGACGCATTATGA

Domains

Predicted by InterproScan.

Type IV Pilus-assembly protein W (PilW)

(154-303)

Prokaryotic N-terminal methylation motif (N_methyl)

(11-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilW Acinetobacter baumannii D1279779

48.036

100

0.515

Multiple sequence alignment