Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   GJS32_RS08175 Genome accession   NZ_CP046040
Coordinates   1725395..1726717 (-) Length   440 a.a.
NCBI ID   WP_014623352.1    Uniprot ID   -
Organism   Streptococcus equi subsp. zooepidemicus strain OH-71905     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1720395..1731717
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJS32_RS08165 (GJS32_08165) hpf 1724131..1724679 (-) 549 WP_014623350.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  GJS32_RS08170 (GJS32_08170) - 1724755..1725381 (-) 627 WP_228269595.1 ComF family protein -
  GJS32_RS08175 (GJS32_08175) comFA/cflA 1725395..1726717 (-) 1323 WP_014623352.1 DEAD/DEAH box helicase Machinery gene
  GJS32_RS08180 (GJS32_08180) - 1726773..1727414 (+) 642 WP_014623353.1 YigZ family protein -
  GJS32_RS08185 (GJS32_08185) cysK 1727535..1728461 (+) 927 WP_014623354.1 cysteine synthase A -
  GJS32_RS08190 (GJS32_08190) - 1728875..1729237 (-) 363 WP_014623356.1 S1 RNA-binding domain-containing protein -
  GJS32_RS08195 (GJS32_08195) - 1729237..1730637 (-) 1401 WP_038674046.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  GJS32_RS08200 (GJS32_08200) - 1730671..1731312 (-) 642 WP_021321066.1 response regulator -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 50003.18 Da        Isoelectric Point: 9.6947

>NTDB_id=400477 GJS32_RS08175 WP_014623352.1 1725395..1726717(-) (comFA/cflA) [Streptococcus equi subsp. zooepidemicus strain OH-71905]
MENIENYYGRLLPERQCPKAVSVWACSLQSMIAKKGTLYCQRCSSLIEKAHQLPSGAYYCRACLVFGRNQSDRPLLYFPP
APFPKAHYLRWQGQLTTYQGAVSHQLTNHVKLKQDTLVHAVTGAGKTEMMYEAIAAVVDKGGWVCIASPRVDVCIELEKR
LSRDFSCQVCLMHAESEVYHRSPIIVATTHQLMTFYHAFDLLIIDEVDAFPFVNNRQLNHAAHQAAKADAVTVYLTATST
RDLERKVKQKELVKLTLARRFHGKPLVVPKYQRLFSLLEAINRGKLPRRFITLVKKQRATGYPLLIFFPLIELAEQCCQL
LQKYFPKETIAHASSQSSNRMAIIEQFRQGQITILISTTILERGVTFPTVDVFVLLANHRLYTSSSLIQISGRVGRSLER
PEGQVLFFHDGISKAMLKAVREIKDMNQKGYQNELSTLPQ

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=400477 GJS32_RS08175 WP_014623352.1 1725395..1726717(-) (comFA/cflA) [Streptococcus equi subsp. zooepidemicus strain OH-71905]
ATGGAGAATATCGAAAATTACTATGGACGTCTTTTACCCGAAAGGCAATGCCCAAAGGCTGTTTCTGTCTGGGCTTGCAG
CTTACAAAGCATGATCGCTAAAAAAGGAACATTATACTGCCAACGCTGTAGCAGCTTAATTGAGAAAGCTCATCAGCTAC
CTAGCGGTGCTTACTATTGTAGAGCCTGTCTCGTTTTTGGTCGAAACCAATCTGATCGGCCCTTGCTCTATTTTCCACCG
GCTCCTTTTCCAAAGGCACATTATCTGAGGTGGCAAGGACAGCTCACAACATATCAGGGAGCTGTCTCTCATCAGCTTAC
TAACCATGTTAAGCTCAAGCAAGACACACTGGTTCATGCCGTTACCGGCGCTGGTAAAACAGAGATGATGTATGAAGCCA
TTGCAGCAGTTGTTGATAAGGGTGGCTGGGTCTGCATTGCTAGTCCACGAGTTGATGTCTGCATAGAGCTTGAAAAACGA
CTATCGCGGGACTTTTCCTGTCAGGTCTGCCTTATGCATGCTGAGTCAGAAGTTTATCATAGAAGTCCCATTATCGTTGC
CACAACACATCAATTGATGACCTTTTACCATGCTTTTGATCTGCTCATTATTGACGAAGTAGATGCCTTCCCTTTTGTCA
ATAATCGTCAATTAAACCATGCTGCTCATCAGGCTGCAAAAGCAGATGCAGTGACAGTATACCTAACAGCAACCTCTACA
AGAGATTTAGAGCGCAAGGTCAAGCAAAAAGAGCTTGTCAAATTGACATTGGCAAGAAGATTTCATGGCAAGCCCTTAGT
TGTTCCAAAGTATCAAAGATTATTCTCCCTTTTAGAGGCTATCAATCGCGGGAAATTGCCTAGAAGGTTCATCACCCTAG
TCAAAAAACAAAGGGCAACTGGCTATCCTCTCTTAATCTTTTTTCCGCTTATTGAGCTGGCTGAGCAATGCTGTCAGCTG
CTCCAAAAGTATTTTCCTAAGGAAACTATTGCCCATGCTTCTAGTCAGTCATCAAATCGAATGGCTATCATTGAGCAATT
CAGACAAGGACAAATCACTATACTTATATCAACAACCATTTTGGAAAGAGGTGTGACCTTTCCGACCGTAGATGTCTTTG
TCTTATTAGCCAATCATCGCCTTTACACAAGCAGCAGTCTTATTCAAATTTCAGGTCGCGTTGGGCGCTCTTTGGAAAGA
CCGGAAGGACAGGTGCTGTTTTTTCATGATGGCATTAGTAAGGCCATGCTCAAGGCAGTGCGGGAGATAAAGGACATGAA
TCAAAAAGGGTATCAAAATGAACTGTCTACTCTGCCACAATAA

Domains

Predicted by InterproScan.

DEAD/DEAH box helicase (DEAD)

(112-244)

Helicase conserved C-terminal domain (Helicase_C)

(288-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae R6

52.706

96.591

0.509
  comFA/cflA Streptococcus pneumoniae Rx1

52.706

96.591

0.509
  comFA/cflA Streptococcus pneumoniae D39

52.706

96.591

0.509
  comFA/cflA Streptococcus pneumoniae TIGR4

52.706

96.591

0.509
  comFA/cflA Streptococcus mitis SK321

52.706

96.591

0.509
  comFA/cflA Streptococcus mitis NCTC 12261

52.471

96.591

0.507
  comFA Lactococcus lactis subsp. cremoris KW2

47.959

89.091

0.427

Multiple sequence alignment