Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   GJS30_RS07005 Genome accession   NZ_CP045993
Coordinates   1373930..1375759 (+) Length   609 a.a.
NCBI ID   WP_094296111.1    Uniprot ID   A0AAP3YFQ4
Organism   Bacillus velezensis strain LABIM22     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1368930..1380759
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJS30_RS06980 (GJS30_06960) - 1369592..1370170 (+) 579 WP_007409110.1 GNAT family N-acetyltransferase -
  GJS30_RS06985 (GJS30_06965) spx 1370349..1370744 (+) 396 WP_003155034.1 transcriptional regulator Spx -
  GJS30_RS06990 (GJS30_06970) - 1370802..1371458 (-) 657 WP_014417421.1 TerC family protein -
  GJS30_RS06995 (GJS30_06975) mecA 1371734..1372390 (+) 657 WP_103527000.1 adaptor protein MecA Regulator
  GJS30_RS07000 (GJS30_06980) - 1372542..1373702 (+) 1161 WP_179155983.1 competence protein CoiA family protein -
  GJS30_RS07005 (GJS30_06985) pepF 1373930..1375759 (+) 1830 WP_094296111.1 oligoendopeptidase F Regulator
  GJS30_RS07010 - 1375797..1375964 (-) 168 WP_003155026.1 hypothetical protein -
  GJS30_RS07015 (GJS30_06990) spxH 1376250..1377152 (-) 903 WP_021494985.1 protease adaptor protein SpxH -
  GJS30_RS07020 (GJS30_06995) - 1377149..1377547 (-) 399 WP_003155023.1 thiol management oxidoreductase -
  GJS30_RS07025 (GJS30_07000) - 1377776..1378462 (-) 687 WP_014721000.1 lytic transglycosylase domain-containing protein -
  GJS30_RS07030 (GJS30_07005) - 1378467..1379039 (-) 573 WP_029973141.1 CYTH domain-containing protein -
  GJS30_RS07035 (GJS30_07010) - 1379164..1379529 (+) 366 WP_012117290.1 hypothetical protein -
  GJS30_RS07040 (GJS30_07015) yjbM 1379557..1380192 (+) 636 WP_007610625.1 GTP diphosphokinase -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 69798.48 Da        Isoelectric Point: 5.3508

>NTDB_id=400047 GJS30_RS07005 WP_094296111.1 1373930..1375759(+) (pepF) [Bacillus velezensis strain LABIM22]
MTKENKAAQLPDRSEVKAEHTWRLEDIFPSDEAWNKEFQAVKELIPNLSSFKGTLAHSADNLYAALTAQDEVTNRLGKLY
AYAHMRSDQDTGNSFYQGLNDKAASLYSQAASATAYIEPEILAIQEDKLHQFILEKEELKLYSHAIEEITKQRPHVLSEQ
EEALLAEASEALSSPSNTFSVLNNADITFPSIKDENGKETQITHGNFINFLESSNREVRKNAFEAVYKTYGQYKNTMATT
LSGTVKKDNFYARVKKYKSAREAALSNNSIPEEVYDNLIKTINKHLPLLHRYIDLRKKVLGLDEVHIYDLYTPLVKDSGM
KVTYEEAKDYMLKGLAPLGEEYASILKEGLENRWVDVYENKGKRSGAYSSGTYGTNPYILMNWHDNVNNLFTLVHEFGHS
VHSYYTRKYQPYPYGNYSIFVAEVASTTNEALLGEYMLNSLKDEKQRLYLLNHMLEGFRGTVFRQTMFAEFEHLIHTKAQ
EGEPLTPELLNELYYDLNKKYFGDNMVIDKEISLEWSRIPHFYYNYYVYQYATGYSAAQALSSQILKEGKPAVERYTEFL
KAGSSEYPIDILKKAGVDMTSPEPIEAACKMFEEKLNEMEELLSKVNPS

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=400047 GJS30_RS07005 WP_094296111.1 1373930..1375759(+) (pepF) [Bacillus velezensis strain LABIM22]
ATGACTAAGGAAAATAAAGCAGCACAGCTGCCTGACAGAAGTGAAGTAAAGGCAGAGCATACATGGAGACTAGAGGATAT
TTTTCCTAGTGATGAGGCCTGGAATAAAGAATTTCAAGCAGTTAAAGAATTAATCCCGAATTTATCTTCTTTTAAAGGCA
CACTTGCTCATTCGGCTGATAATTTATACGCGGCGCTTACGGCACAGGATGAAGTGACGAACCGCCTTGGGAAACTTTAT
GCATACGCGCATATGCGCTCAGACCAGGATACGGGAAATTCTTTCTACCAAGGGCTGAACGACAAAGCCGCAAGCCTGTA
TTCTCAGGCTGCAAGCGCGACTGCTTACATTGAACCGGAAATTCTGGCGATTCAGGAGGACAAGCTGCACCAGTTTATTC
TCGAAAAAGAAGAGCTTAAGCTTTACTCCCATGCAATTGAAGAAATTACAAAACAGCGTCCGCACGTGCTGAGCGAACAA
GAAGAGGCCCTGCTTGCGGAAGCCTCAGAAGCGCTGTCGTCGCCTTCCAATACATTCAGCGTTTTAAATAACGCCGATAT
TACATTTCCGTCGATTAAAGATGAAAACGGCAAGGAAACTCAAATCACACACGGCAACTTTATTAATTTCCTGGAAAGCA
GCAATCGTGAAGTCCGCAAAAACGCATTTGAAGCCGTATATAAAACATACGGACAGTATAAAAACACGATGGCGACTACA
CTCAGCGGCACTGTGAAAAAAGATAATTTTTACGCCCGTGTGAAAAAATATAAATCAGCACGGGAAGCGGCTTTATCCAA
TAACAGCATCCCTGAAGAAGTTTACGATAACCTGATTAAAACGATCAATAAACATCTTCCGTTATTACACCGTTACATTG
ACCTGCGGAAAAAGGTGCTCGGCCTTGATGAAGTCCATATTTATGACCTGTACACACCGCTCGTAAAAGATTCGGGAATG
AAGGTCACATATGAGGAAGCGAAAGATTATATGCTCAAAGGACTTGCTCCGCTGGGCGAGGAATACGCGTCTATTTTAAA
AGAAGGACTTGAAAACCGCTGGGTCGATGTATACGAAAACAAAGGAAAACGCAGCGGTGCTTATTCGTCCGGTACATACG
GCACGAATCCGTACATCTTGATGAACTGGCATGATAATGTCAATAATCTGTTTACGCTCGTTCACGAGTTCGGCCATTCC
GTGCACAGCTACTATACGAGAAAATATCAGCCGTATCCATACGGTAACTACAGCATTTTTGTCGCTGAAGTCGCTTCAAC
GACCAATGAAGCGCTGCTCGGTGAATATATGCTGAACAGCTTAAAGGATGAAAAACAGCGTCTGTACCTGCTCAACCACA
TGCTTGAAGGATTCAGAGGCACAGTGTTCAGACAGACGATGTTCGCTGAATTTGAGCATCTGATTCATACGAAAGCGCAG
GAAGGCGAGCCGCTGACGCCTGAACTGCTGAACGAACTTTATTATGATCTGAATAAAAAGTATTTCGGTGACAACATGGT
GATTGATAAAGAAATCAGTCTGGAATGGTCAAGAATTCCGCATTTCTATTACAATTATTATGTGTATCAATATGCAACAG
GCTACAGTGCGGCCCAAGCGCTGAGCAGCCAGATCTTAAAAGAAGGCAAGCCTGCGGTGGAGCGCTATACCGAATTCTTA
AAAGCGGGAAGCTCGGAATATCCGATCGACATACTGAAAAAAGCGGGAGTCGATATGACATCGCCTGAACCGATCGAGGC
GGCCTGCAAAATGTTTGAAGAGAAGCTGAACGAAATGGAAGAGCTTCTTTCGAAAGTGAACCCTTCGTAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(120-189)

Peptidase family M3 (Peptidase_M3)

(211-590)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

50

97.537

0.488

Multiple sequence alignment