Detailed information NTG0400012

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: CEA74_RS20995
• Genome accession: NZ_CP045988
• Coordinates: 4317578..4318378 (+)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain WCHKP35
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4312578..4323378

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CEA74_RS20960 (CEA74_20640)	gspH	4312734..4313249 (+)	516	WP_004213437.1	type II secretion system minor pseudopilin GspH	-
CEA74_RS20965 (CEA74_20645)	gspI	4313246..4313611 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
CEA74_RS20970 (CEA74_20650)	gspJ	4313608..4314204 (+)	597	WP_004222700.1	type II secretion system minor pseudopilin GspJ	-
CEA74_RS20975 (CEA74_20655)	gspK	4314201..4315187 (+)	987	WP_004222697.1	type II secretion system minor pseudopilin GspK	-
CEA74_RS20980 (CEA74_20660)	gspL	4315174..4316367 (+)	1194	WP_004191957.1	type II secretion system protein GspL	-
CEA74_RS20985 (CEA74_20665)	-	4316360..4316845 (+)	486	WP_004151943.1	type II secretion system protein M	-
CEA74_RS20990 (CEA74_20670)	-	4316835..4317581 (+)	747	WP_004145897.1	type II secretion system protein N	-
CEA74_RS20995 (CEA74_20675)	pilD	4317578..4318378 (+)	801	WP_002888947.1	A24 family peptidase	Machinery gene
CEA74_RS21000 (CEA74_20680)	hrpB	4318426..4320855 (-)	2430	WP_020317204.1	ATP-dependent helicase HrpB	-
CEA74_RS21005 (CEA74_20685)	thpR	4320928..4321464 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
CEA74_RS21010 (CEA74_20690)	sfsA	4321464..4322180 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
CEA74_RS21015 (CEA74_20695)	dksA	4322343..4322798 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=400012 CEA74_RS20995 WP_002888947.1 4317578..4318378(+) (pilD) [Klebsiella pneumoniae strain WCHKP35]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=400012 CEA74_RS20995 WP_002888947.1 4317578..4318378(+) (pilD) [Klebsiella pneumoniae strain WCHKP35]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425