Detailed information NTG0399760

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GJE04_RS08250
• Genome accession: NZ_CP045961
• Coordinates: 1562972..1563832 (+)
• Length: 286 a.a.
• NCBI ID: WP_002241067.1
• Uniprot ID: A1KVV2
• Organism: Neisseria meningitidis strain AUSMDU00010537
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1557972..1568832

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GJE04_RS08230 (GJE04_08230)	dapD	1558482..1559303 (-)	822	WP_002220281.1	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase	-
GJE04_RS08235 (GJE04_08235)	-	1559313..1559543 (-)	231	WP_025462060.1	hypothetical protein	-
GJE04_RS08240 (GJE04_08240)	pgi	1559513..1561156 (-)	1644	WP_002247959.1	glucose-6-phosphate isomerase	-
GJE04_RS08245 (GJE04_08245)	pilG	1561666..1562898 (+)	1233	WP_002216405.1	type 4 pilus assembly protein PilG	Machinery gene
GJE04_RS08250 (GJE04_08250)	pilD	1562972..1563832 (+)	861	WP_002241067.1	prepilin peptidase	Machinery gene
GJE04_RS08255 (GJE04_08255)	coaE	1563834..1564466 (+)	633	WP_002216402.1	dephospho-CoA kinase	-
GJE04_RS08260 (GJE04_08260)	-	1564459..1564668 (+)	210	WP_002216401.1	DNA gyrase inhibitor YacG	-
GJE04_RS08265 (GJE04_08265)	pilF	1564753..1566429 (-)	1677	WP_002221967.1	type IV-A pilus assembly ATPase PilB	Machinery gene
GJE04_RS08280 (GJE04_08280)	-	1566921..1567367 (-)	447	WP_002220271.1	DUF441 domain-containing protein	-
GJE04_RS08285 (GJE04_08285)	ispB	1567372..1568346 (-)	975	WP_002247958.1	octaprenyl diphosphate synthase	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 31331.48 Da
• Isoelectric Point: 9.1920

>NTDB_id=399760 GJE04_RS08250 WP_002241067.1 1562972..1563832(+) (pilD) [Neisseria meningitidis strain AUSMDU00010537]
MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVFAKKHLNLPLTEKESRTFNLMKPDSCCPKCRVPIRAW
QNIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLV
AAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRAVNWWLTHPVL

Nucleotide

• Length: 861 bp

>NTDB_id=399760 GJE04_RS08250 WP_002241067.1 1562972..1563832(+) (pilD) [Neisseria meningitidis strain AUSMDU00010537]
ATGTCTGATTTGTCTGTATTGTCGCCGTTTGCCGTGCCTTTGGCAGCGGTGTTCGGGCTGCTGGTCGGCAGTTTCTTAAA
CGTCGTCATTTACCGCGTGCCGGTCATGATGGAACGCGGCTGGACGGTATTTGCCAAAAAACATTTAAACCTGCCGCTGA
CCGAAAAGGAAAGCCGTACCTTCAACCTGATGAAACCGGATTCCTGCTGTCCCAAATGCCGCGTGCCGATACGCGCGTGG
CAGAACATCCCGATTGTCAGCTACCTGCTCCTGCGCGGCAAATGCGCTTCCTGCCAAACCAAAATCAGCATACGTTATCC
CTTAATCGAGCTGCTGACCGGCGTATTATTCGGGCTGGTCGCCTGGCAATACGGCTGGTCTTGGATTACATTGGGCGGTT
TGATACTGACCGCGTTTCTGATTTCCCTGACCTTTATCGATGCGGACACCCAATACCTGCCCGACTCGATGACACTGCCC
TTGATTTGGCTGGGGCTGATATTTAATTTGGACGGCGGCTTCGTGCCTTTGCAGTCTGCCGTTTTAGGTGCGGTTGCCGG
CTATGGTTCGATATGGCTTTTATGCGCGGTATATAAACTGATTAAAGGCGAAATCGGTATGGGCAACGGAGATTTCAAAC
TGATTGCCGCATTGGGCGCGTGGCTCGGCATATCCGCATTGCCCGTGCTGATTTTTGTTTCCTCGCTGATCGGTTTGGTC
GCGGCAATCGTTATGCGCGTCGCCAAGGGGCAGCATTTTGCCTTCGGCCCCGCACTGACAGTTTCGGGCTGGATAATTTT
TACGGCAAACGATTCCGTATGGCGGGCGGTCAACTGGTGGCTGACCCATCCGGTGCTGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (134-242)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

3D structure

• AlphaFold DB: A1KVV2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Neisseria gonorrhoeae MS11	96.14	99.65	0.958
pilD	Vibrio cholerae strain A1552	44.444	100	0.448
pilD	Acinetobacter baumannii D1279779	46.97	92.308	0.434
pilD	Acinetobacter nosocomialis M2	46.97	92.308	0.434
pilD	Vibrio campbellii strain DS40M4	44.565	96.503	0.43