Detailed information NTG0399256

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: GI367_RS08215
• Genome accession: NZ_CP045926
• Coordinates: 1765203..1765817 (-)
• Length: 204 a.a.
• NCBI ID: WP_007612692.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain AL7
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1760203..1770817

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GI367_RS08185 (GI367_08185)	rpsT	1760506..1760772 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
GI367_RS08190 (GI367_08190)	holA	1760789..1761830 (-)	1042	Protein_1633	DNA polymerase III subunit delta	-
GI367_RS08195 (GI367_08195)	-	1761870..1762021 (-)	152	Protein_1634	hypothetical protein	-
GI367_RS08200 (GI367_08200)	-	1762062..1762196 (+)	135	WP_029326064.1	YqzM family protein	-
GI367_RS08205 (GI367_08205)	comEC	1762215..1764566 (-)	2352	WP_007612689.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
GI367_RS08210 (GI367_08210)	-	1764567..1765136 (-)	570	WP_003152868.1	ComE operon protein 2	-
GI367_RS08215 (GI367_08215)	comEA	1765203..1765817 (-)	615	WP_007612692.1	helix-hairpin-helix domain-containing protein	Machinery gene
GI367_RS08220 (GI367_08220)	comER	1765876..1766697 (+)	822	WP_007612693.1	late competence protein ComER	-
GI367_RS08225 (GI367_08225)	-	1766766..1767503 (-)	738	WP_007612694.1	class I SAM-dependent DNA methyltransferase	-
GI367_RS08230 (GI367_08230)	rsfS	1767500..1767856 (-)	357	WP_007408260.1	ribosome silencing factor	-
GI367_RS08235 (GI367_08235)	yqeK	1767874..1768434 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
GI367_RS08240 (GI367_08240)	-	1768424..1768993 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
GI367_RS08245 (GI367_08245)	yhbY	1769004..1769294 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
GI367_RS08250 (GI367_08250)	aroE	1769288..1770130 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22265.52 Da
• Isoelectric Point: 7.7754

>NTDB_id=399256 GI367_RS08215 WP_007612692.1 1765203..1765817(-) (comEA) [Bacillus velezensis strain AL7]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELKVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=399256 GI367_RS08215 WP_007612692.1 1765203..1765817(-) (comEA) [Bacillus velezensis strain AL7]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAAAGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.115	93.627	0.544
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.071	100	0.363