Detailed information NTG0399057

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GIY05_RS25460
• Genome accession: NZ_CP045916
• Coordinates: 5464422..5465294 (+)
• Length: 290 a.a.
• NCBI ID: WP_003107301.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain CF39S
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5459422..5470294

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GIY05_RS25435	-	5459609..5459935 (-)	327	WP_346777756.1	pilin	-
GIY05_RS25440	-	5460015..5460995 (-)	981	WP_003284896.1	IS5-like element ISPst7 family transposase	-
GIY05_RS25445	-	5461057..5461269 (-)	213	Protein_5037	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
GIY05_RS25450	pilB	5461500..5463200 (+)	1701	WP_003107297.1	type IV-A pilus assembly ATPase PilB	Machinery gene
GIY05_RS25455	pilC	5463204..5464421 (+)	1218	WP_003107299.1	type II secretion system F family protein	Machinery gene
GIY05_RS25460	pilD	5464422..5465294 (+)	873	WP_003107301.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
GIY05_RS25465	coaE	5465291..5465902 (+)	612	WP_003094654.1	dephospho-CoA kinase	-
GIY05_RS25470	yacG	5465899..5466099 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
GIY05_RS25475	-	5466136..5466345 (-)	210	WP_020750398.1	hypothetical protein	-
GIY05_RS25480	-	5466451..5467140 (-)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
GIY05_RS25485	-	5467137..5467607 (-)	471	WP_003103866.1	hypothetical protein	-
GIY05_RS25490	-	5467604..5468029 (-)	426	WP_003103865.1	GNAT family N-acetyltransferase	-
GIY05_RS25495	-	5468162..5468791 (+)	630	WP_003094668.1	DUF1780 domain-containing protein	-
GIY05_RS25500	-	5468788..5469237 (+)	450	WP_003094670.1	MOSC domain-containing protein	-
GIY05_RS25505	-	5469263..5469433 (+)	171	WP_003094672.1	DUF3094 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31873.90 Da
• Isoelectric Point: 7.7068

>NTDB_id=399057 GIY05_RS25460 WP_003107301.1 5464422..5465294(+) (pilD) [Pseudomonas aeruginosa strain CF39S]
MTLLDYLASHPLAFVLCTILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=399057 GIY05_RS25460 WP_003107301.1 5464422..5465294(+) (pilD) [Pseudomonas aeruginosa strain CF39S]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCACCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAAGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.357	96.552	0.534
pilD	Vibrio campbellii strain DS40M4	52.65	97.586	0.514
pilD	Acinetobacter nosocomialis M2	50.53	97.586	0.493
pilD	Acinetobacter baumannii D1279779	50.53	97.586	0.493
pilD	Neisseria gonorrhoeae MS11	48.921	95.862	0.469