Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   APZ19_RS02505 Genome accession   NZ_CP045859
Coordinates   507514..508554 (+) Length   346 a.a.
NCBI ID   WP_005436511.1    Uniprot ID   A0A0C1VE31
Organism   Vibrio owensii strain SH14     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 502514..513554
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  APZ19_RS02470 (APZ19_02470) hemW 502535..503707 (-) 1173 WP_054824847.1 radical SAM family heme chaperone HemW -
  APZ19_RS02475 (APZ19_02475) - 503726..504328 (-) 603 WP_054824846.1 XTP/dITP diphosphatase -
  APZ19_RS02480 (APZ19_02480) - 504434..504865 (-) 432 WP_054824845.1 DUF4426 domain-containing protein -
  APZ19_RS02485 (APZ19_02485) yggU 504925..505215 (-) 291 WP_005425805.1 DUF167 family protein YggU -
  APZ19_RS02490 (APZ19_02490) - 505215..505772 (-) 558 WP_005425806.1 YggT family protein -
  APZ19_RS02495 (APZ19_02495) proC 505826..506644 (-) 819 WP_054824844.1 pyrroline-5-carboxylate reductase -
  APZ19_RS02500 (APZ19_02500) - 506775..507485 (-) 711 WP_005436513.1 YggS family pyridoxal phosphate-dependent enzyme -
  APZ19_RS02505 (APZ19_02505) pilT 507514..508554 (+) 1041 WP_005436511.1 type IV pilus twitching motility protein PilT Machinery gene
  APZ19_RS02510 (APZ19_02510) pilU 508586..509692 (+) 1107 WP_005436509.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  APZ19_RS02515 (APZ19_02515) ruvX 509754..510179 (-) 426 WP_005436506.1 Holliday junction resolvase RuvX -
  APZ19_RS02520 (APZ19_02520) - 510231..510794 (-) 564 WP_005436504.1 YqgE/AlgH family protein -
  APZ19_RS02525 (APZ19_02525) gshB 510923..511873 (-) 951 WP_054824843.1 glutathione synthase -
  APZ19_RS02530 (APZ19_02530) rsmE 511888..512619 (-) 732 WP_050909845.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  APZ19_RS02535 (APZ19_02535) endA 512766..513461 (-) 696 WP_020197218.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38661.14 Da        Isoelectric Point: 6.5043

>NTDB_id=398743 APZ19_RS02505 WP_005436511.1 507514..508554(+) (pilT) [Vibrio owensii strain SH14]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFRKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHILTIED
PIEFVHNNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVSREEVDKKVEIEAQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=398743 APZ19_RS02505 WP_005436511.1 507514..508554(+) (pilT) [Vibrio owensii strain SH14]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCTTCGGATCTACATCTTTCTGCAGGTGTACCACCAAT
GGTTCGTATTGATGGTGATGTGAGAAAGCTGGGCATTCCAGCGTTTACACACCAAGAAGTGCACCGTTTGGTTTTTGAAA
TCATGAACGATGCGCAGCGCAGTGAGTTCGAAGAAAAACTCGAAGTCGATTTCTCATTTGAATTGCATAACGTTGGCCGT
TTTCGTGTCAACGCTTTCAACCAATCTCGCGGTTGTGCGGCTGTGTTCCGTACCATCCCAAGCAGCATTCCAACACTGGA
AGAGCTAGAAGCTCCAGAGATCTTCAGAAAAATCGCTAACGCCGAGAAAGGTCTGGTTCTGGTTACTGGCCCAACGGGTT
CTGGTAAATCGACGACCTTGGCTGCCATTGTTGACTACATTAACCGTAATCACAATAAGCACATCCTAACAATTGAAGAT
CCAATTGAATTTGTCCACAACAACAATAAGTGTCTGATCAACCAACGTGAAGTTCACCGTGATACACACAGCTTCCAAAA
CGCGCTGCGTAGCGCATTGCGTGAAGACCCGGATGTGATTCTAGTCGGTGAGATGCGTGACAAAGAAACCATCAGTCTAG
CGCTGACAGCGGCTGAAACTGGTCACCTTGTTTTCGGTACACTACACACGAGCTCTGCGGCGAAAACCATCGACCGTATT
ATCGATGTTTTCCCGGGGAGCGACAAAGACATGGTGCGCTCGATGTTGTCGGAATCGCTGCGTTCGGTTATCGCGCAAAA
GCTTTTGAAACGTAACGGCGGTGGTCGTATTGCGTGTCATGAAATCATGATGGCAACACCGGCGATCCGAAACCTTATTC
GTGAAGATAAAGTCGCGCAGATGTACTCGATCATTCAGACCGGTGCAGCGCACGGCATGCAGACCATGGAACAAAATGCT
CGTCAATTGATGGCGCAAGGCATGGTGTCGCGTGAAGAAGTGGACAAGAAAGTTGAAATTGAAGCGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C1VE31

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.667

99.711

0.864
  pilT Vibrio cholerae strain A1552

86.667

99.711

0.864
  pilT Acinetobacter baumannii D1279779

72.424

95.376

0.691
  pilT Acinetobacter baumannii strain A118

72.424

95.376

0.691
  pilT Acinetobacter nosocomialis M2

72.121

95.376

0.688
  pilT Acinetobacter baylyi ADP1

71.818

95.376

0.685
  pilT Pseudomonas stutzeri DSM 10701

69.322

97.977

0.679
  pilT Pseudomonas aeruginosa PAK

69.118

98.266

0.679
  pilT Legionella pneumophila strain Lp02

67.976

95.665

0.65
  pilT Legionella pneumophila strain ERS1305867

67.976

95.665

0.65
  pilT Neisseria meningitidis 8013

64.412

98.266

0.633
  pilT Neisseria gonorrhoeae MS11

64.118

98.266

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.143

100

0.506
  pilU Vibrio cholerae strain A1552

41.916

96.532

0.405
  pilU Pseudomonas stutzeri DSM 10701

40.719

96.532

0.393
  pilU Acinetobacter baylyi ADP1

39.062

92.486

0.361

Multiple sequence alignment