Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GII77_RS02035 Genome accession   NZ_CP045825
Coordinates   376942..377391 (-) Length   149 a.a.
NCBI ID   WP_003234608.1    Uniprot ID   A0A8E0S750
Organism   Bacillus subtilis strain 75     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 371942..382391
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GII77_RS01995 (GII77_01995) - 372396..372584 (+) 189 Protein_347 hypothetical protein -
  GII77_RS02000 (GII77_02000) - 372620..372935 (+) 316 Protein_348 RDD family protein -
  GII77_RS02005 (GII77_02005) yckD 373017..373349 (+) 333 WP_015252849.1 YckD family protein -
  GII77_RS02010 (GII77_02010) - 373477..373899 (+) 423 WP_174616579.1 MarR family transcriptional regulator -
  GII77_RS02015 (GII77_02015) - 373887..374228 (+) 342 WP_032724911.1 DUF3147 family protein -
  GII77_RS02020 (GII77_02020) - 374247..374974 (+) 728 Protein_352 AAA family ATPase -
  GII77_RS02025 (GII77_02025) bglC 375047..376480 (+) 1434 WP_015252846.1 6-phospho-beta-glucosidase -
  GII77_RS02030 (GII77_02030) nin/comJ 376517..376915 (-) 399 WP_015252845.1 DNA-entry nuclease inhibitor Regulator
  GII77_RS02035 (GII77_02035) nucA/comI 376942..377391 (-) 450 WP_003234608.1 DNA-entry nuclease Machinery gene
  GII77_RS02040 (GII77_02040) tlpC 377559..379280 (-) 1722 WP_015252843.1 methyl-accepting chemotaxis protein TlpC -
  GII77_RS02045 (GII77_02045) hxlB 379391..379948 (-) 558 WP_015252842.1 6-phospho-3-hexuloisomerase -
  GII77_RS02050 (GII77_02050) hxlA 379954..380586 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  GII77_RS02055 (GII77_02055) hxlR 380818..381180 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16470.42 Da        Isoelectric Point: 4.5282

>NTDB_id=398271 GII77_RS02035 WP_003234608.1 376942..377391(-) (nucA/comI) [Bacillus subtilis strain 75]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSTDQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=398271 GII77_RS02035 WP_003234608.1 376942..377391(-) (nucA/comI) [Bacillus subtilis strain 75]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGTCTGATAAA
AGGAGACTTTTTTTCAACTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGACACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment