Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GII85_RS13020 Genome accession   NZ_CP045817
Coordinates   2538268..2538678 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain P5_B1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2533268..2543678
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GII85_RS12990 (GII85_12990) - 2533355..2533495 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  GII85_RS12995 (GII85_12995) yqeF 2533800..2534531 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  GII85_RS13000 (GII85_13000) cwlH 2534783..2535535 (-) 753 WP_021480067.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  GII85_RS13005 (GII85_13005) yqeD 2535722..2536348 (+) 627 WP_041850032.1 TVP38/TMEM64 family protein -
  GII85_RS13010 (GII85_13010) gnd 2536368..2537261 (-) 894 WP_041850033.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  GII85_RS13015 (GII85_13015) yqeB 2537513..2538235 (+) 723 WP_032726205.1 hypothetical protein -
  GII85_RS13020 (GII85_13020) nucA/comI 2538268..2538678 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  GII85_RS13025 (GII85_13025) sigK 2538874..2539602 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  GII85_RS13030 (GII85_13030) - 2539602..2539718 (+) 117 WP_041850087.1 hypothetical protein -
  GII85_RS13035 (GII85_13035) - 2539684..2539881 (-) 198 Protein_2532 recombinase family protein -
  GII85_RS13040 (GII85_13040) - 2539839..2540003 (-) 165 WP_080332536.1 hypothetical protein -
  GII85_RS13045 (GII85_13045) psiE 2540548..2540964 (-) 417 WP_003246154.1 phosphate-starvation-inducible protein PsiE -
  GII85_RS13050 (GII85_13050) yrkQ 2541010..2542308 (-) 1299 WP_041850088.1 two-component system sensor histidine kinase YrkQ -
  GII85_RS13055 (GII85_13055) yrkP 2542295..2542990 (-) 696 WP_041850089.1 two-component response regulator YrkP -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=397754 GII85_RS13020 WP_009967785.1 2538268..2538678(-) (nucA/comI) [Bacillus subtilis strain P5_B1]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=397754 GII85_RS13020 WP_009967785.1 2538268..2538678(-) (nucA/comI) [Bacillus subtilis strain P5_B1]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCAATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment