Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   GII85_RS12940 Genome accession   NZ_CP045817
Coordinates   2526394..2527011 (-) Length   205 a.a.
NCBI ID   WP_041850029.1    Uniprot ID   -
Organism   Bacillus subtilis strain P5_B1     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2521394..2532011
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GII85_RS12910 (GII85_12910) rpsT 2521696..2521962 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  GII85_RS12915 (GII85_12915) holA 2521977..2523020 (-) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  GII85_RS12920 (GII85_12920) - 2523060..2523209 (-) 150 WP_120363468.1 hypothetical protein -
  GII85_RS12925 (GII85_12925) yqzM 2523250..2523384 (+) 135 WP_003229983.1 YqzM family protein -
  GII85_RS12930 (GII85_12930) comEC 2523424..2525754 (-) 2331 WP_041850028.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  GII85_RS12935 (GII85_12935) comEB 2525758..2526327 (-) 570 WP_003229978.1 ComE operon protein 2 -
  GII85_RS12940 (GII85_12940) comEA 2526394..2527011 (-) 618 WP_041850029.1 competence protein ComEA Machinery gene
  GII85_RS12945 (GII85_12945) comER 2527095..2527916 (+) 822 WP_041850030.1 late competence protein ComER -
  GII85_RS12950 (GII85_12950) yqeM 2527982..2528725 (-) 744 WP_032726197.1 class I SAM-dependent methyltransferase -
  GII85_RS12955 (GII85_12955) rsfS 2528722..2529078 (-) 357 WP_003237112.1 ribosome silencing factor -
  GII85_RS12960 (GII85_12960) yqeK 2529096..2529656 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  GII85_RS12965 (GII85_12965) nadD 2529646..2530215 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  GII85_RS12970 (GII85_12970) yhbY 2530227..2530517 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  GII85_RS12975 (GII85_12975) aroE 2530511..2531353 (-) 843 WP_041850031.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21821.62 Da        Isoelectric Point: 4.7220

>NTDB_id=397753 GII85_RS12940 WP_041850029.1 2526394..2527011(-) (comEA) [Bacillus subtilis strain P5_B1]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEVQVNLAEILQDGIVVYIPKKGEETAVQQGGGVSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=397753 GII85_RS12940 WP_041850029.1 2526394..2527011(-) (comEA) [Bacillus subtilis strain P5_B1]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCAATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGTGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGATAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGTGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATATCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-117)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

98.049

100

0.98
  comEA Staphylococcus aureus MW2

36.818

100

0.395
  comEA Staphylococcus aureus N315

35.455

100

0.38

Multiple sequence alignment