Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GII86_RS02195 Genome accession   NZ_CP045816
Coordinates   401458..401907 (-) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis strain P5_B2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 396458..406907
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GII86_RS02155 (GII86_02155) - 396898..397095 (+) 198 Protein_382 hypothetical protein -
  GII86_RS02160 (GII86_02160) - 397132..397452 (+) 321 Protein_383 RDD family protein -
  GII86_RS02165 (GII86_02165) yckD 397534..397866 (+) 333 WP_046160038.1 YckD family protein -
  GII86_RS02170 (GII86_02170) - 397993..398415 (+) 423 WP_174616579.1 MarR family transcriptional regulator -
  GII86_RS02175 (GII86_02175) - 398403..398744 (+) 342 WP_032724911.1 DUF3147 family protein -
  GII86_RS02180 (GII86_02180) - 398763..399491 (+) 729 WP_046160039.1 AAA family ATPase -
  GII86_RS02185 (GII86_02185) bglC 399563..400996 (+) 1434 WP_046160040.1 6-phospho-beta-glucosidase -
  GII86_RS02190 (GII86_02190) nin/comJ 401033..401431 (-) 399 WP_021481824.1 DNA-entry nuclease inhibitor Regulator
  GII86_RS02195 (GII86_02195) nucA/comI 401458..401907 (-) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  GII86_RS02200 (GII86_02200) tlpC 402075..403796 (-) 1722 WP_046160041.1 methyl-accepting chemotaxis protein TlpC -
  GII86_RS02205 (GII86_02205) hxlB 403907..404464 (-) 558 WP_046160042.1 6-phospho-3-hexuloisomerase -
  GII86_RS02210 (GII86_02210) hxlA 404470..405102 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  GII86_RS02215 (GII86_02215) hxlR 405334..405696 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=397630 GII86_RS02195 WP_014475770.1 401458..401907(-) (nucA/comI) [Bacillus subtilis strain P5_B2]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=397630 GII86_RS02195 WP_014475770.1 401458..401907(-) (nucA/comI) [Bacillus subtilis strain P5_B2]
GTGAACATCACGACGGACATCATAAAAACAATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCTTCTGACCGTT
ATCCCGAAACGGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987

Multiple sequence alignment