Detailed information NTG0397544

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: GII89_RS20150
• Genome accession: NZ_CP045812
• Coordinates: 3845616..3846761 (+)
• Length: 381 a.a.
• NCBI ID: WP_003227549.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain P8_B3
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3840616..3851761

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GII89_RS20125 (GII89_20125)	albF	3840632..3841912 (+)	1281	WP_003242974.1	pitrilysin family protein	-
GII89_RS20130 (GII89_20130)	albG	3841909..3842610 (+)	702	WP_003243391.1	antilisterial bacteriocin subtilosin biosynthesis protein AlbG	-
GII89_RS20135 (GII89_20135)	ywhL	3842616..3843992 (-)	1377	WP_003244415.1	YncE family protein	-
GII89_RS20140 (GII89_20140)	ywhK	3844031..3845386 (-)	1356	WP_003227552.1	YncE family protein	-
GII89_RS22860 (GII89_20145)	-	3845383..3845613 (-)	231	WP_009968328.1	hypothetical protein	-
GII89_RS20150 (GII89_20150)	rapF	3845616..3846761 (+)	1146	WP_003227549.1	response regulator aspartate phosphatase RapF	Regulator
GII89_RS20155 (GII89_20155)	phrF	3846745..3846864 (+)	120	WP_009968329.1	phosphatase RapF inhibitor PhrF	Regulator
GII89_RS20160 (GII89_20160)	ywhH	3846963..3847436 (+)	474	WP_003227547.1	YbaK/EbsC family protein	-
GII89_RS20165 (GII89_20165)	speB	3847468..3848340 (-)	873	WP_003227545.1	agmatinase	-
GII89_RS20170 (GII89_20170)	speE	3848401..3849231 (-)	831	WP_003227543.1	spermidine synthase	-
GII89_RS20175 (GII89_20175)	pbpG	3849433..3851508 (+)	2076	WP_003227540.1	transglycosylase domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45609.09 Da
• Isoelectric Point: 5.1503

>NTDB_id=397544 GII89_RS20150 WP_003227549.1 3845616..3846761(+) (rapF) [Bacillus subtilis strain P8_B3]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKEHEAYNIRLLQCHSLFATNFLDLKQYEDAISHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AIPYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKIDKAHEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFAIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=397544 GII89_RS20150 WP_003227549.1 3845616..3846761(+) (rapF) [Bacillus subtilis strain P8_B3]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTAATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAACTTCTTCAG
AGGCATGTACGAGCTGGACCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAAAAGGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAACATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCATCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGGAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATACCTTATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATAGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGGAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCGCTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (223-290)

• Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3) (132-208)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	100	100	1
rapC	Bacillus subtilis subsp. subtilis str. 168	57.368	99.738	0.572