Detailed information    

insolico Bioinformatically predicted

Overview

Name   phrF   Type   Regulator
Locus tag   GII89_RS20155 Genome accession   NZ_CP045812
Coordinates   3846745..3846864 (+) Length   39 a.a.
NCBI ID   WP_009968329.1    Uniprot ID   A0A199WEM2
Organism   Bacillus subtilis strain P8_B3     
Function   antagonize RapF (predicted from homology)   
Competence regulation

Genomic Context

Location: 3841745..3851864
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GII89_RS20130 (GII89_20130) albG 3841909..3842610 (+) 702 WP_003243391.1 antilisterial bacteriocin subtilosin biosynthesis protein AlbG -
  GII89_RS20135 (GII89_20135) ywhL 3842616..3843992 (-) 1377 WP_003244415.1 YncE family protein -
  GII89_RS20140 (GII89_20140) ywhK 3844031..3845386 (-) 1356 WP_003227552.1 YncE family protein -
  GII89_RS22860 (GII89_20145) - 3845383..3845613 (-) 231 WP_009968328.1 hypothetical protein -
  GII89_RS20150 (GII89_20150) rapF 3845616..3846761 (+) 1146 WP_003227549.1 response regulator aspartate phosphatase RapF Regulator
  GII89_RS20155 (GII89_20155) phrF 3846745..3846864 (+) 120 WP_009968329.1 phosphatase RapF inhibitor PhrF Regulator
  GII89_RS20160 (GII89_20160) ywhH 3846963..3847436 (+) 474 WP_003227547.1 YbaK/EbsC family protein -
  GII89_RS20165 (GII89_20165) speB 3847468..3848340 (-) 873 WP_003227545.1 agmatinase -
  GII89_RS20170 (GII89_20170) speE 3848401..3849231 (-) 831 WP_003227543.1 spermidine synthase -
  GII89_RS20175 (GII89_20175) pbpG 3849433..3851508 (+) 2076 WP_003227540.1 transglycosylase domain-containing protein -

Sequence

Protein


Download         Length: 39 a.a.        Molecular weight: 4199.10 Da        Isoelectric Point: 10.4997

>NTDB_id=397545 GII89_RS20155 WP_009968329.1 3846745..3846864(+) (phrF) [Bacillus subtilis strain P8_B3]
MKLKSKLLLSCLALSTVFVATTIANAPTHQIEVAQRGMI

Nucleotide


Download         Length: 120 bp        

>NTDB_id=397545 GII89_RS20155 WP_009968329.1 3846745..3846864(+) (phrF) [Bacillus subtilis strain P8_B3]
ATGAAATTGAAGTCTAAACTATTACTCTCTTGTCTGGCTCTAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACC
TACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAA

Domains

Predicted by InterproScan.

Rap-phr extracellular signalling (PhrC_PhrF)

(3-39)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A199WEM2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  phrF Bacillus subtilis subsp. subtilis str. 168

100

100

1
  phrC Bacillus subtilis subsp. subtilis str. 168

48.718

100

0.487

Multiple sequence alignment