Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   N028_RS03470 Genome accession   NZ_CP045799
Coordinates   760984..762576 (-) Length   530 a.a.
NCBI ID   WP_003403505.1    Uniprot ID   A0A0L8IK44
Organism   Pseudomonas syringae USA011     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 755984..767576
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N028_RS03450 (N028_03450) - 757994..758401 (+) 408 WP_024639448.1 type IV pilin protein -
  N028_RS03455 (N028_03455) thiO 758427..759530 (+) 1104 WP_024639449.1 glycine oxidase ThiO -
  N028_RS03465 (N028_03465) pilR 759644..760981 (-) 1338 WP_024639450.1 sigma-54-dependent transcriptional regulator Regulator
  N028_RS03470 (N028_03470) pilS 760984..762576 (-) 1593 WP_003403505.1 sensor histidine kinase Regulator
  N028_RS03475 (N028_03475) - 762566..762805 (-) 240 WP_032611259.1 PP0621 family protein -
  N028_RS03480 (N028_03480) - 762926..763948 (-) 1023 WP_003371138.1 outer membrane protein assembly factor BamD -
  N028_RS03485 (N028_03485) rluD 764098..765060 (+) 963 WP_024639452.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  N028_RS03490 (N028_03490) pgeF 765057..765785 (+) 729 WP_024639453.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 57986.92 Da        Isoelectric Point: 7.0011

>NTDB_id=397298 N028_RS03470 WP_003403505.1 760984..762576(-) (pilS) [Pseudomonas syringae USA011]
MTADSPSLVSPQAQRILRLYHLYRLTIGVVLVLLISSSLETELLQLANPNLFRSGCWLYLVFNILVVVLLERPSRQAQFF
GLAMTDAIMLSALFYAAGGPSSGIGNLLIASVAISNVLLRGRIGLLIAAVSAIGIIYLTFYISFGTPSVSNDYVQAGSLG
ALCFAAALLVQALTARMQVSEVLAEQRAADVDSLEALNALILQRMRTGILVLDANHQVLLANQGALHLLGQEQLVGQIID
TCSPQLVERLRQWLVNPIMVPRSITTAPVGLVLQPSFATLNRGDQRQTLVFLEDLSQIAHQAQQLKLAALGRLTAGIAHE
IRNPLGAVSHAAQLLNESEELSAADRRLGQIIHEQSGRIDRVIENVLQLSRRREAQPELLDLKTWLEHFVAEFRSSAAPN
QKIHVDISPGTLTTRMDSGQLTQIMTNLVQNGLRYSGKNHPSAQVWLKLHNDQKSDLPTLEVLDDGPGVSEQHLSKMFEP
FFTTESKGTGLGLYLSRELCESNQAHLEYAPRPTGGSCLRITFAHPFKTS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=397298 N028_RS03470 WP_003403505.1 760984..762576(-) (pilS) [Pseudomonas syringae USA011]
GTGACCGCTGACTCACCGTCCCTTGTCAGCCCGCAGGCACAGCGGATTCTACGGCTGTACCACCTCTACCGACTGACCAT
CGGCGTTGTACTGGTTCTGTTGATTTCCAGCAGCCTGGAAACCGAGCTGCTGCAACTGGCCAACCCGAACCTGTTCCGCT
CGGGCTGCTGGTTGTATCTGGTGTTCAACATTCTGGTCGTCGTGTTGCTGGAACGGCCCAGCCGTCAGGCACAGTTTTTC
GGCCTGGCGATGACCGACGCGATCATGCTCTCCGCGCTATTCTACGCAGCCGGCGGGCCTTCCAGCGGTATCGGCAACCT
GTTGATTGCCTCAGTGGCGATCAGCAACGTGCTGCTGCGCGGCCGTATCGGCTTGTTGATCGCTGCGGTTTCGGCCATCG
GCATCATCTACCTGACGTTCTACATCAGCTTCGGCACACCCTCGGTTTCCAACGACTACGTGCAGGCTGGCTCGCTGGGC
GCCTTGTGCTTCGCCGCCGCCCTGCTGGTGCAGGCGCTGACAGCGCGCATGCAGGTCAGCGAGGTACTGGCCGAACAGCG
CGCGGCGGATGTCGACAGCCTCGAAGCACTCAACGCACTCATTCTGCAACGCATGCGTACCGGCATTCTGGTGCTGGATG
CCAATCACCAGGTGCTGCTGGCCAATCAGGGTGCCTTACACCTGCTGGGTCAGGAGCAACTGGTCGGCCAGATCATCGAC
ACCTGCTCGCCACAACTGGTTGAACGCCTGCGCCAGTGGCTCGTCAACCCGATCATGGTGCCGCGCAGCATCACCACCGC
CCCCGTCGGGCTGGTACTGCAACCGAGTTTTGCCACACTCAACCGTGGCGATCAGCGTCAGACGCTGGTTTTTCTCGAAG
ACCTGTCGCAGATCGCCCATCAGGCGCAGCAGCTCAAGCTTGCCGCACTGGGGCGCCTGACCGCCGGGATTGCCCATGAG
ATACGCAACCCGCTGGGCGCCGTCAGTCATGCGGCGCAGCTGCTTAATGAATCGGAAGAGCTCAGTGCGGCGGATCGACG
CTTGGGGCAGATCATTCATGAGCAGTCGGGACGCATTGATAGGGTCATCGAAAATGTCCTGCAGTTGTCACGCAGACGCG
AAGCGCAACCCGAACTGCTCGATCTGAAAACCTGGCTGGAACACTTTGTCGCTGAATTTCGCAGTTCCGCTGCGCCCAAT
CAGAAGATTCATGTCGACATCAGCCCTGGCACGCTGACAACCCGCATGGATTCTGGCCAACTGACGCAAATCATGACCAA
CCTGGTGCAGAACGGCCTGCGCTACAGCGGCAAGAATCATCCGTCAGCGCAGGTCTGGCTGAAACTGCACAATGACCAGA
AGAGCGACCTGCCCACACTGGAAGTGCTGGACGACGGGCCGGGGGTCAGCGAGCAACACCTGAGCAAAATGTTCGAACCG
TTTTTCACCACAGAAAGCAAAGGCACCGGCCTTGGTCTATATCTGTCACGTGAACTGTGCGAAAGCAATCAGGCGCATCT
GGAATATGCGCCCCGCCCCACGGGTGGCAGTTGCCTGCGCATTACCTTCGCCCACCCGTTCAAGACGAGCTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)

PAS domain (PAS_8)

(202-254)

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0L8IK44

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

61.017

100

0.611

Multiple sequence alignment