Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   GH799_RS04810 Genome accession   NZ_CP045794
Coordinates   954041..954619 (+) Length   192 a.a.
NCBI ID   WP_005496569.1    Uniprot ID   A0AAX0M4K7
Organism   Vibrio parahaemolyticus 10329     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 949041..959619
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GH799_RS04795 (GH799_04790) oxyR 949364..950272 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  GH799_RS04800 (GH799_04795) - 950328..952898 (-) 2571 WP_005496567.1 PBP1A family penicillin-binding protein -
  GH799_RS04805 (GH799_04800) pilM 953041..954057 (+) 1017 WP_005496568.1 type IV pilus assembly protein PilM Machinery gene
  GH799_RS04810 (GH799_04805) pilN 954041..954619 (+) 579 WP_005496569.1 PilN domain-containing protein Machinery gene
  GH799_RS04815 (GH799_04810) pilO 954612..955205 (+) 594 WP_005496571.1 type 4a pilus biogenesis protein PilO Machinery gene
  GH799_RS04820 (GH799_04815) pilP 955195..955710 (+) 516 WP_005454781.1 pilus assembly protein PilP Machinery gene
  GH799_RS04825 (GH799_04820) pilQ 955742..957490 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ family protein Machinery gene
  GH799_RS04830 (GH799_04825) aroK 957679..958197 (+) 519 WP_005381319.1 shikimate kinase AroK -
  GH799_RS04835 (GH799_04830) aroB 958229..959329 (+) 1101 WP_005496576.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 192 a.a.        Molecular weight: 22478.98 Da        Isoelectric Point: 7.0769

>NTDB_id=397234 GH799_RS04810 WP_005496569.1 954041..954619(+) (pilN) [Vibrio parahaemolyticus 10329]
MLYRINLLHWRENQREEHRRRFVGLVVLGVMVALGIQWGVGKYYEYQQDLQQERLDYLTTYISDLDKRIEAMKIAELEHS
KILERLKVVEALQNGRNKTTDFMNLMPAVIPEGVYVDKIKMNDLEIEISGISDSTPRLATMLDNMERSAKLLDVEMHSIV
HGKARFGKEFQTFKVSFMFNTVEEKKEGKQHG

Nucleotide


Download         Length: 579 bp        

>NTDB_id=397234 GH799_RS04810 WP_005496569.1 954041..954619(+) (pilN) [Vibrio parahaemolyticus 10329]
GTGCTGTATCGCATTAACTTATTGCATTGGCGCGAAAATCAAAGGGAAGAGCATCGTCGTCGATTTGTCGGTTTAGTGGT
ATTGGGCGTCATGGTAGCGTTGGGGATTCAGTGGGGCGTTGGCAAATATTATGAGTACCAACAAGATCTTCAACAAGAGC
GACTCGACTATCTAACAACGTACATTTCCGATCTAGATAAGCGGATAGAAGCGATGAAGATCGCGGAGCTTGAACACAGC
AAAATCTTGGAACGCTTGAAAGTTGTGGAAGCGTTGCAAAATGGTCGGAATAAAACCACTGATTTTATGAACCTCATGCC
TGCTGTTATTCCAGAAGGCGTGTATGTCGACAAGATCAAAATGAACGATCTAGAAATTGAAATCTCAGGTATTTCAGACA
GTACACCTCGGCTAGCAACCATGCTCGATAACATGGAGCGTTCTGCCAAGCTACTGGACGTTGAAATGCACTCTATCGTG
CACGGAAAAGCCCGTTTCGGTAAAGAGTTTCAGACCTTTAAAGTGTCCTTCATGTTCAACACGGTAGAAGAGAAGAAAGA
GGGAAAACAACATGGTTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

83.333

100

0.833
  pilN Vibrio cholerae strain A1552

62.431

94.271

0.589

Multiple sequence alignment