Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LG045_RS07645 Genome accession   NZ_CP045729
Coordinates   1507356..1508729 (-) Length   457 a.a.
NCBI ID   WP_056937702.1    Uniprot ID   -
Organism   Limosilactobacillus gastricus strain LG045     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1502356..1513729
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG045_RS07620 (LG045_07620) rlmB 1502496..1503251 (-) 756 WP_056937697.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  LG045_RS07625 (LG045_07625) - 1503241..1503657 (-) 417 WP_056937698.1 Mini-ribonuclease 3 -
  LG045_RS07630 (LG045_07630) cysS 1503661..1505070 (-) 1410 WP_056937699.1 cysteine--tRNA ligase -
  LG045_RS07635 (LG045_07635) - 1505445..1505750 (+) 306 WP_056937700.1 hypothetical protein -
  LG045_RS07640 (LG045_07640) gltX 1505782..1507281 (-) 1500 WP_056937701.1 glutamate--tRNA ligase -
  LG045_RS07645 (LG045_07645) radA 1507356..1508729 (-) 1374 WP_056937702.1 DNA repair protein RadA Machinery gene
  LG045_RS07650 (LG045_07650) - 1508745..1509280 (-) 536 Protein_1491 dUTP diphosphatase -
  LG045_RS07655 (LG045_07655) - 1509423..1509713 (+) 291 WP_056937704.1 GNAT family N-acetyltransferase -
  LG045_RS07660 (LG045_07660) rpiA 1509730..1510419 (+) 690 WP_056937705.1 ribose-5-phosphate isomerase RpiA -
  LG045_RS07665 (LG045_07665) rcrQ 1510480..1512240 (-) 1761 WP_056937728.1 ABC transporter ATP-binding protein Regulator

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49538.91 Da        Isoelectric Point: 8.7241

>NTDB_id=396418 LG045_RS07645 WP_056937702.1 1507356..1508729(-) (radA) [Limosilactobacillus gastricus strain LG045]
MAKAKVHYVCQNCGYNSPRFLGRCPNCGEWNTLVEKVEQTSVAAATKATTTLTGIVARPQKINEIDTKKTPRVKTKLREL
NRVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSGQLSLENHPVLYISGEESANQIKMRAQRIAVSGDNFYIYPETNMDSIR
ATIDNLKPEYVVIDSVQTMQASGVESAIGSVAQIRAVTAQLMQIAKGENITVFVVGHVTKGGTIAGPKILEHMVDTVLYF
EGDSHHTYRILRSVKNRFGSTNELGIFEMHDTGLTEVANPSEMFLEERLKDATGSAVVVSLEGTRPILVEVQALITPTVF
GNAQRTASGLDRNRVSLIMAVLEKRANLLLQNQDAYLQAAGGVKLNEPAIDLALAISIASSYRDKGTRPTDAFIGELGLT
GEVRRVSRIEQRVAEAAKLGFQRVFIPKNNLQHWTPPTNIEVVGVATINEALKLALS

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=396418 LG045_RS07645 WP_056937702.1 1507356..1508729(-) (radA) [Limosilactobacillus gastricus strain LG045]
ATGGCGAAAGCAAAAGTGCATTATGTTTGCCAGAACTGTGGTTATAATTCACCCCGCTTCTTAGGGCGTTGTCCAAATTG
TGGTGAGTGGAATACTCTAGTTGAAAAGGTGGAACAAACCAGTGTGGCTGCAGCGACTAAGGCTACAACCACTTTGACGG
GGATTGTGGCTCGTCCCCAAAAAATTAATGAGATTGATACGAAAAAGACGCCACGGGTTAAAACTAAATTACGCGAATTA
AATCGGGTCCTAGGTGGCGGGATTGTACCAGGTTCGTTAGTGTTGATTGGGGGAGATCCTGGGATTGGGAAATCGACCCT
CTTGCTACAAGTCTCTGGACAGCTTAGTCTGGAAAATCACCCCGTTTTATATATTTCGGGGGAAGAAAGTGCGAATCAAA
TCAAGATGCGGGCTCAACGAATTGCAGTTTCGGGAGACAATTTTTATATTTATCCAGAAACCAATATGGATAGCATTCGC
GCTACGATTGATAATTTGAAACCTGAATATGTGGTTATTGATTCGGTCCAAACCATGCAAGCATCAGGAGTTGAATCAGC
CATTGGGAGTGTCGCGCAAATCCGAGCCGTCACCGCTCAATTAATGCAGATTGCTAAGGGGGAAAACATTACGGTGTTCG
TGGTCGGCCATGTAACCAAAGGTGGAACGATTGCGGGTCCTAAAATTTTGGAACACATGGTTGATACGGTCCTTTATTTT
GAGGGGGACTCTCATCATACTTACCGGATTTTACGGTCGGTTAAAAATCGGTTTGGCTCCACTAATGAATTAGGCATCTT
CGAGATGCATGATACGGGATTAACTGAGGTGGCGAATCCCTCGGAGATGTTTTTGGAAGAACGCTTAAAAGATGCTACCG
GATCGGCAGTAGTTGTTTCCTTGGAAGGGACGCGGCCGATTCTAGTAGAGGTGCAAGCCCTGATTACGCCTACCGTATTT
GGGAATGCCCAGCGAACCGCATCAGGTTTGGATCGAAATCGGGTTTCGTTAATCATGGCTGTTTTGGAAAAACGGGCTAA
TTTACTCTTACAAAATCAGGATGCCTATTTACAAGCGGCTGGGGGCGTAAAGTTAAACGAACCAGCGATTGATCTAGCCC
TAGCCATCAGTATTGCTTCGAGTTATCGCGACAAAGGGACCCGACCAACGGATGCTTTTATTGGTGAATTAGGTTTGACG
GGGGAAGTTCGGCGAGTAAGTCGTATTGAACAACGAGTGGCCGAAGCCGCTAAATTGGGTTTTCAACGGGTCTTTATCCC
GAAAAATAATTTGCAACATTGGACACCACCGACCAATATTGAAGTGGTTGGGGTTGCCACTATTAATGAAGCTTTAAAAT
TAGCTTTATCTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

64.901

99.125

0.643
  radA Streptococcus mitis NCTC 12261

64.68

99.125

0.641
  radA Streptococcus pneumoniae Rx1

64.68

99.125

0.641
  radA Streptococcus pneumoniae D39

64.68

99.125

0.641
  radA Streptococcus pneumoniae R6

64.68

99.125

0.641
  radA Streptococcus pneumoniae TIGR4

64.68

99.125

0.641
  radA Bacillus subtilis subsp. subtilis str. 168

62.719

99.781

0.626

Multiple sequence alignment