Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilK   Type   Machinery gene
Locus tag   GHB96_RS02320 Genome accession   NZ_CP045707
Coordinates   449567..450166 (+) Length   199 a.a.
NCBI ID   WP_050157381.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain NG250     
Function   type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 444567..455166
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GHB96_RS02295 (GHB96_02415) - 444896..445477 (-) 582 WP_003698180.1 superoxide dismutase -
  GHB96_RS02300 (GHB96_02420) dnaB 445640..447046 (+) 1407 WP_215772538.1 replicative DNA helicase -
  GHB96_RS02305 (GHB96_02425) pilH 447354..448016 (+) 663 WP_215772539.1 Tfp pilus assembly protein FimT/FimU Machinery gene
  GHB96_RS02310 (GHB96_02430) pilI 448048..448656 (+) 609 WP_215772540.1 type IV pilus modification protein PilV Machinery gene
  GHB96_RS02315 (GHB96_02435) pilJ 448653..449588 (+) 936 WP_215772541.1 PilW family protein Machinery gene
  GHB96_RS02320 (GHB96_02440) pilK 449567..450166 (+) 600 WP_050157381.1 pilus assembly protein Machinery gene
  GHB96_RS02325 (GHB96_02445) pilL 450168..450641 (+) 474 WP_215772556.1 PilX family type IV pilin Machinery gene
  GHB96_RS02330 (GHB96_02450) - 450711..451019 (-) 309 WP_025456241.1 AzlD family protein -
  GHB96_RS02335 (GHB96_02455) - 451016..451727 (-) 712 Protein_459 AzlC family ABC transporter permease -
  GHB96_RS02340 (GHB96_02460) dut 451893..452345 (+) 453 WP_003687923.1 dUTP diphosphatase -
  GHB96_RS02345 (GHB96_02465) dapC 452423..453610 (+) 1188 WP_003687924.1 succinyldiaminopimelate transaminase -
  GHB96_RS02350 (GHB96_02470) yaaA 453921..454700 (+) 780 WP_003687925.1 peroxide stress protein YaaA -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 21709.74 Da        Isoelectric Point: 8.4516

>NTDB_id=396153 GHB96_RS02320 WP_050157381.1 449567..450166(+) (pilK) [Neisseria gonorrhoeae strain NG250]
MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNTEQRISANESDRKLALSLAEAALREGEFQVLDLEYAA
DSKVTFSENCEKGLCTAVNVRTNNNGNEEAFGNIVVKGKPIVEAVKRSCPANSASLCIDNRGMEYKKGTAGVSKMPRYII
EYLGVKNGQNVYRVTAKAWGKNANTVVVLQSYVGNNDEQ

Nucleotide


Download         Length: 600 bp        

>NTDB_id=396153 GHB96_RS02320 WP_050157381.1 449567..450166(+) (pilK) [Neisseria gonorrhoeae strain NG250]
ATGCGCAAACAGAACACTTTGACAGGAATCCCGACTTCTGACGGACAGAGGGGGTTCGCACTGTTTATCGTGCTGATGGT
GATGATAGTCGTGGCCTTTTTGGTTGTAACTGCCGCCCAGTCCTACAATACCGAACAGAGGATCAGTGCCAACGAATCAG
ACAGGAAATTGGCTTTGTCTTTAGCCGAGGCGGCTTTGCGGGAGGGCGAATTTCAGGTTTTGGATTTGGAATATGCTGCG
GATAGTAAGGTTACATTTAGCGAAAACTGTGAAAAAGGCCTGTGTACCGCAGTGAATGTGCGGACAAATAATAATGGTAA
TGAAGAGGCTTTTGGCAATATCGTGGTGAAAGGCAAGCCCATCGTTGAGGCGGTGAAGCGTTCTTGCCCTGCAAATTCTG
CCAGCCTGTGCATTGACAATAGAGGGATGGAATATAAGAAAGGCACGGCAGGCGTCAGCAAAATGCCGCGCTATATTATC
GAATATTTAGGCGTGAAGAACGGACAAAATGTTTATCGGGTTACTGCCAAGGCTTGGGGTAAGAATGCCAATACCGTGGT
CGTCCTTCAATCTTATGTAGGCAATAATGATGAGCAATAA

Domains

Predicted by InterProScan.

Type IV pilus assembly protein PilX C-term (PilX)

(92-194)

PilX N-terminal (PilX_N)

(17-67)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilK Neisseria gonorrhoeae MS11

90.148

100

0.92

Multiple sequence alignment