Detailed information NTG0396024

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GHA50_RS22355
• Genome accession: NZ_CP045694
• Coordinates: 4335127..4335927 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain TK421
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4330127..4340927

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GHA50_RS22320 (GHA50_22320)	gspH	4330283..4330798 (+)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-
GHA50_RS22325 (GHA50_22325)	gspI	4330795..4331160 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
GHA50_RS22330 (GHA50_22330)	gspJ	4331157..4331753 (+)	597	WP_023279845.1	type II secretion system minor pseudopilin GspJ	-
GHA50_RS22335 (GHA50_22335)	gspK	4331750..4332736 (+)	987	WP_004177385.1	type II secretion system minor pseudopilin GspK	-
GHA50_RS22340 (GHA50_22340)	gspL	4332723..4333916 (+)	1194	WP_025985182.1	type II secretion system protein GspL	-
GHA50_RS22345 (GHA50_22345)	-	4333909..4334394 (+)	486	WP_153459431.1	type II secretion system protein M	-
GHA50_RS22350 (GHA50_22350)	-	4334384..4335130 (+)	747	WP_004145897.1	type II secretion system protein N	-
GHA50_RS22355 (GHA50_22355)	pilD	4335127..4335927 (+)	801	WP_004145898.1	prepilin peptidase	Machinery gene
GHA50_RS22360 (GHA50_22360)	hrpB	4335975..4338404 (-)	2430	WP_023302300.1	ATP-dependent helicase HrpB	-
GHA50_RS22365 (GHA50_22365)	thpR	4338477..4339013 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
GHA50_RS22370 (GHA50_22370)	sfsA	4339013..4339729 (+)	717	WP_004177390.1	DNA/RNA nuclease SfsA	-
GHA50_RS22375 (GHA50_22375)	dksA	4339892..4340347 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=396024 GHA50_RS22355 WP_004145898.1 4335127..4335927(+) (pilD) [Klebsiella pneumoniae strain TK421]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=396024 GHA50_RS22355 WP_004145898.1 4335127..4335927(+) (pilD) [Klebsiella pneumoniae strain TK421]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425