Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   GE376_RS21520 Genome accession   NZ_CP045606
Coordinates   4267586..4268629 (-) Length   347 a.a.
NCBI ID   WP_001013177.1    Uniprot ID   -
Organism   Bacillus cereus strain SB1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4262586..4273629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GE376_RS21475 (GE376_21960) - 4263020..4263220 (-) 201 WP_000106083.1 YqzE family protein -
  GE376_RS21480 (GE376_21965) aroK 4263259..4263756 (-) 498 WP_000836649.1 shikimate kinase AroK -
  GE376_RS21485 (GE376_21970) - 4263872..4264522 (-) 651 WP_000183853.1 2OG-Fe(II) oxygenase -
  GE376_RS21490 (GE376_21975) comGG 4264696..4265067 (-) 372 WP_023523011.1 competence type IV pilus minor pilin ComGG -
  GE376_RS21495 (GE376_21980) comGF 4265064..4265534 (-) 471 WP_000923097.1 competence type IV pilus minor pilin ComGF -
  GE376_RS21500 (GE376_21985) - 4265504..4265806 (-) 303 WP_000829462.1 hypothetical protein -
  GE376_RS21505 (GE376_21990) comGD 4265799..4266254 (-) 456 WP_002094140.1 competence type IV pilus minor pilin ComGD -
  GE376_RS21510 (GE376_21995) comGC 4266251..4266550 (-) 300 WP_001178687.1 competence type IV pilus major pilin ComGC -
  GE376_RS21515 (GE376_22000) comGB 4266562..4267593 (-) 1032 WP_023523013.1 competence type IV pilus assembly protein ComGB -
  GE376_RS21520 (GE376_22005) comGA 4267586..4268629 (-) 1044 WP_001013177.1 competence type IV pilus ATPase ComGA Machinery gene
  GE376_RS21525 (GE376_22010) - 4268835..4269530 (+) 696 WP_000434124.1 helix-turn-helix transcriptional regulator -
  GE376_RS21530 (GE376_22015) - 4269657..4269899 (+) 243 WP_000375619.1 DUF2626 domain-containing protein -
  GE376_RS21535 (GE376_22020) - 4270007..4271401 (+) 1395 WP_001094344.1 L-cystine transporter -
  GE376_RS31925 - 4271481..4271873 (-) 393 Protein_4264 hypothetical protein -
  GE376_RS21545 (GE376_22030) - 4271961..4272176 (-) 216 WP_001008326.1 DUF3912 family protein -
  GE376_RS21550 (GE376_22035) - 4272430..4272741 (+) 312 WP_001093240.1 hypothetical protein -
  GE376_RS21555 (GE376_22040) - 4272776..4273264 (-) 489 WP_000764263.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39177.60 Da        Isoelectric Point: 8.8944

>NTDB_id=395644 GE376_RS21520 WP_001013177.1 4267586..4268629(-) (comGA) [Bacillus cereus strain SB1]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRHCIKKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEADGEC
VTYKHETLESSIRKGYALGFLEDDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=395644 GE376_RS21520 WP_001013177.1 4267586..4268629(-) (comGA) [Bacillus cereus strain SB1]
ATGAATGGGATTGAAATTTTTGCGAATACAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGAGACATTGTATTAAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTTAAATTTTTAGCATCTATGGACATAGGAGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTGCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTCGTGTTTACTGGACCGACTGGTTCTGGGAAGACAACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTCATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCAAGCATTTATGAGCTGCTATATGGATATGAGTTAAAACAAGCGCTTAAAGAAGCAGATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATAAGAAAAGGATATGCTTTAGGTTTTTTAGAAGATGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.196

100

0.562
  pilB Haemophilus influenzae 86-028NP

39.752

92.795

0.369

Multiple sequence alignment