Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   GE569_RS03750 Genome accession   NZ_CP045576
Coordinates   803330..804835 (-) Length   501 a.a.
NCBI ID   WP_013699407.1    Uniprot ID   A0A560VPW2
Organism   Burkholderia gladioli strain KACC 18963     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 798330..809835
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GE569_RS03725 (GE569_03715) - 799321..799956 (-) 636 WP_013699412.1 LON peptidase substrate-binding domain-containing protein -
  GE569_RS03730 (GE569_03720) rapZ 799989..800882 (-) 894 WP_013699411.1 RNase adapter RapZ -
  GE569_RS03735 (GE569_03725) hprK 800993..801961 (-) 969 WP_013699410.1 HPr(Ser) kinase/phosphatase -
  GE569_RS03740 (GE569_03730) - 802087..802599 (-) 513 WP_096750181.1 PTS sugar transporter subunit IIA -
  GE569_RS03745 (GE569_03735) raiA 802814..803179 (-) 366 WP_013699408.1 ribosome-associated translation inhibitor RaiA -
  GE569_RS03750 (GE569_03740) rpoN/rpoN1 803330..804835 (-) 1506 WP_013699407.1 RNA polymerase factor sigma-54 Machinery gene
  GE569_RS03755 (GE569_03745) lptB 805026..805802 (-) 777 WP_013699406.1 LPS export ABC transporter ATP-binding protein -
  GE569_RS03760 (GE569_03750) lptA 805799..806467 (-) 669 WP_013699405.1 lipopolysaccharide transport periplasmic protein LptA -
  GE569_RS03765 (GE569_03755) lptC 806486..807100 (-) 615 WP_017918294.1 LPS export ABC transporter periplasmic protein LptC -
  GE569_RS03770 (GE569_03760) - 807104..807643 (-) 540 WP_098153593.1 HAD family hydrolase -
  GE569_RS03775 (GE569_03765) kdsD 807643..808626 (-) 984 WP_186110957.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 55047.01 Da        Isoelectric Point: 4.9483

>NTDB_id=395294 GE569_RS03750 WP_013699407.1 803330..804835(-) (rpoN/rpoN1) [Burkholderia gladioli strain KACC 18963]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDEWIASPLRVAADGSLIAQSPPSSTPEPMHG
GGSNGSEASGDRAERDDSRNGDDYDYNADSGDSGQWNLDDYGRSSGASDDDDLPPLQVHEASTSLREHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLGAGLDEVLTDLPEELEVDLDELGAALALLHSFDPAGVGARSASECLRLQLLRLDPSPTRTL
SLDIVSQHLELLAARDFTRLRKQLKANDDELREAHALIRSLEPFPGAAYGKTEADYVVPDIMVRKSGQNWLAELNPEVVP
KLRINHLYANILRNSRGDPGAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDQKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=395294 GE569_RS03750 WP_013699407.1 803330..804835(-) (rpoN/rpoN1) [Burkholderia gladioli strain KACC 18963]
ATGAAAGCCAGCCTCCAACTTCGCCTGTCGCAGCACCTCGCGCTCACGCCCCAGCTCCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCCCAGAATCCGCTGCTCGAGAACGACGAATGGATCG
CGAGCCCGCTGCGGGTGGCCGCCGACGGCTCGCTGATCGCGCAGAGCCCGCCTTCCTCGACGCCCGAGCCGATGCACGGC
GGCGGCTCGAACGGCAGCGAAGCCTCGGGCGATCGCGCCGAACGCGACGACTCGCGCAATGGCGACGACTACGACTACAA
CGCCGACAGCGGCGATTCGGGTCAGTGGAATCTCGACGACTACGGCCGCTCCTCGGGCGCCTCCGACGATGACGACCTCC
CGCCGCTGCAGGTGCACGAGGCCTCGACCTCGCTGCGCGAGCATCTGTCCGCGCAATTGCGGGTGACCCAGGCGGGTCCG
CGCGACCGCGCGCTGGTGATGTTCCTGATCGAATCGCTCGACGACGACGGTTATCTCGGCGCGGGGCTCGACGAGGTGTT
GACCGACCTGCCCGAGGAACTGGAGGTCGATCTCGACGAACTGGGCGCCGCGCTGGCGCTGCTGCACAGCTTCGACCCGG
CCGGCGTGGGGGCGCGCTCCGCATCAGAATGCTTGCGCCTGCAATTGTTGCGACTCGATCCGTCCCCCACGCGCACGCTG
TCGCTCGACATCGTCTCGCAGCATCTCGAATTGCTGGCCGCGCGCGACTTCACGCGGCTGCGCAAGCAGTTGAAGGCCAA
CGACGACGAGCTGCGCGAGGCCCATGCACTGATCCGCTCGCTCGAGCCCTTCCCGGGCGCCGCCTACGGCAAGACCGAGG
CGGACTACGTGGTGCCCGACATCATGGTCAGGAAGAGCGGCCAGAACTGGCTCGCGGAGCTCAATCCCGAGGTGGTGCCG
AAGCTGCGCATCAACCACCTGTACGCCAATATCCTGCGCAACAGCCGGGGCGATCCGGGCGCCGGTTCGCTCAAGCAGCA
GCTCCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGATTCGAGACCATCCTGCGTGTCGCGCAGGCCATCGTGG
AGCGTCAGAAGAACTTTTTTGCGCATGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACTCTTGAATTTAAGTACTT
CTTCGGATCGCACGTGTCGACGGATACCGGGGGCGCTGCCTCTTCCACGGCAATCCGCGCCCTCATCAAGCAACTGATAG
GAGCCGAAGACCAGAAATCTCCTCTTTCGGACAGCCGAATAGCCGAGCTGCTGGCGGAACAAGGCTTCGTGGTCGCGCGC
CGCACCGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCCGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A560VPW2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.379

100

0.691

Multiple sequence alignment