Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   HPY16_RS11265 Genome accession   NZ_CP053802
Coordinates   2515638..2517365 (-) Length   575 a.a.
NCBI ID   WP_032478787.1    Uniprot ID   -
Organism   Vibrio cholerae strain L6G     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2510638..2522365
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY16_RS11230 (HPY16_11225) - 2510909..2511679 (-) 771 WP_000086312.1 PilW family protein -
  HPY16_RS11235 (HPY16_11230) - 2511679..2512245 (-) 567 WP_042989135.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HPY16_RS11240 (HPY16_11235) - 2512235..2512747 (-) 513 WP_142546692.1 type II secretion system protein -
  HPY16_RS11245 (HPY16_11240) - 2512811..2513281 (-) 471 WP_000828592.1 type II secretion system protein -
  HPY16_RS11250 (HPY16_11245) - 2513316..2513870 (-) 555 WP_000738708.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HPY16_RS11255 (HPY16_11250) - 2513951..2514403 (-) 453 WP_001960380.1 hypothetical protein -
  HPY16_RS11260 (HPY16_11255) - 2514406..2515629 (-) 1224 WP_057555914.1 type II secretion system F family protein -
  HPY16_RS11265 (HPY16_11260) pilB/pilB1 2515638..2517365 (-) 1728 WP_032478787.1 GspE/PulE family protein Machinery gene
  HPY16_RS11270 (HPY16_11265) - 2517355..2518506 (-) 1152 WP_000117835.1 lipopolysaccharide assembly protein LapB -
  HPY16_RS11275 (HPY16_11270) - 2518503..2519348 (-) 846 WP_000281360.1 ExeA family protein -
  HPY16_RS11280 (HPY16_11275) mshL 2519353..2521016 (-) 1664 Protein_2173 pilus (MSHA type) biogenesis protein MshL -
  HPY16_RS11285 (HPY16_11280) - 2521031..2521348 (-) 318 WP_000252811.1 MSHA biogenesis protein MshK -
  HPY16_RS11290 (HPY16_11285) gspM 2521341..2521991 (-) 651 WP_000835144.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63858.32 Da        Isoelectric Point: 6.0455

>NTDB_id=395082 HPY16_RS11265 WP_032478787.1 2515638..2517365(-) (pilB/pilB1) [Vibrio cholerae strain L6G]
MPINKLRKRLGDLLVEEGIVSEAQLEQALNAQKNTGRRLGDTLISLGFLSETQLLNFLAQQLSLPVIDLSRANVDIDAVP
LLPEVHARRLRALVIGRSGDTLRIAMSDPADLFAQEALLNHLPDYGFEFVIAPEKQLVDGFDRYYRRTKEIVSFAEQLHA
EHKTNDSFDFEITDSDSDEVTVVKLINSLFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLHEVNIASALVLRLKL
MANLDISEKRLPQDGRFNIRVKGQSVDIRMSTMPIQHGESVVMRLLNQSAGVRKLEESGIPPHLLLRLRHQLKRPHGMIL
VTGPTGSGKTTTLYGALNELNTSGKKIITAEDPVEYRISRINQVQVNPKINLDFSTLLRTFLRQDPDIILVGEMRDQETV
EIGLRAALTGHLVLSTLHTNDAVDSALRLIDMGAPGYLVASAVRAVVAQRLVRKVCPDCSGHDEVDEARRQWLVTRFPNQ
VAAKFTRGRGCQNCNLTGYRGRIGVFEMLELDQPMMDCLRANDAVAFSKAARSNTDYKPLLASAMELALQGIVSLDEVMS
LGEGDSSGLVEPIYL

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=395082 HPY16_RS11265 WP_032478787.1 2515638..2517365(-) (pilB/pilB1) [Vibrio cholerae strain L6G]
GTGCCAATTAATAAACTGCGTAAACGGCTTGGTGACTTGCTGGTTGAAGAGGGGATTGTCAGCGAAGCGCAGCTTGAACA
AGCGCTCAATGCCCAGAAAAATACCGGACGCCGCTTAGGGGATACCTTAATTTCGCTTGGCTTTTTAAGCGAAACCCAGT
TGCTGAACTTTTTGGCGCAGCAATTGAGTTTACCTGTGATCGATTTGAGCCGCGCTAATGTTGATATTGATGCGGTTCCT
TTACTGCCTGAAGTTCATGCCCGCCGTTTGCGGGCGCTGGTGATAGGCCGCAGTGGTGATACGCTGCGCATAGCCATGAG
TGATCCTGCGGATCTGTTTGCTCAAGAAGCCTTGCTCAACCACTTGCCTGATTACGGTTTTGAGTTTGTCATCGCTCCTG
AAAAGCAGTTGGTCGATGGCTTTGATCGTTACTATCGCCGCACCAAAGAAATTGTCTCGTTTGCCGAGCAATTGCATGCC
GAACACAAAACCAATGATAGTTTTGATTTTGAGATCACCGATTCAGATAGCGATGAAGTGACGGTTGTTAAACTGATCAA
CTCGCTGTTTGAAGATGCGATTCAAGTGGGTGCCTCGGATATACACATTGAGCCAGATGCCAACGTACTGCGTTTGCGCC
AACGGATCGATGGGGTATTGCATGAAACCTTGCTTCATGAAGTGAATATCGCCTCTGCCTTAGTGCTGCGCTTAAAATTA
ATGGCGAATCTGGATATTTCAGAAAAACGCCTTCCGCAAGATGGACGTTTTAACATCCGTGTAAAAGGACAATCCGTCGA
TATCCGGATGTCAACCATGCCTATCCAGCACGGTGAATCGGTGGTGATGCGTTTGCTCAACCAATCTGCAGGGGTTAGAA
AGCTCGAAGAGTCGGGTATTCCGCCTCATTTATTGCTGCGTTTGCGCCATCAGTTGAAACGCCCTCATGGCATGATTCTG
GTGACTGGGCCTACTGGCTCGGGGAAAACCACCACCTTGTATGGCGCACTCAACGAGCTCAATACGTCCGGCAAAAAAAT
CATTACTGCGGAAGATCCGGTGGAATACCGGATTTCACGGATCAATCAGGTGCAAGTGAACCCGAAAATCAATCTCGATT
TCTCGACGTTACTGCGCACTTTCTTGCGCCAAGACCCAGATATTATTCTGGTCGGTGAGATGCGTGACCAAGAGACGGTC
GAAATTGGCCTGCGCGCTGCCTTAACCGGTCACTTAGTCTTAAGTACTCTGCACACCAATGATGCGGTCGATAGCGCGCT
GCGTTTGATTGATATGGGGGCGCCCGGTTATCTGGTTGCCAGTGCGGTACGTGCCGTGGTTGCGCAGCGTTTAGTACGAA
AAGTCTGTCCGGATTGTAGTGGACATGATGAGGTGGATGAAGCGCGTCGCCAATGGCTTGTGACGCGTTTCCCTAATCAA
GTTGCCGCCAAATTCACTCGTGGACGCGGCTGTCAAAACTGTAACTTAACTGGTTATCGCGGCCGGATCGGTGTATTTGA
AATGCTTGAGCTGGATCAGCCGATGATGGATTGCTTGCGGGCAAATGATGCTGTGGCCTTCTCTAAAGCCGCACGCAGTA
ACACAGATTATAAACCGCTACTGGCTTCGGCGATGGAGTTAGCGCTGCAAGGTATCGTCAGTCTTGATGAAGTGATGTCG
CTGGGTGAAGGTGATTCCTCTGGTTTGGTTGAACCGATTTATCTGTAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-149)

Type II/IV secretion system protein (T2SSE)

(189-454)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

35.596

100

0.374
  pilB Vibrio campbellii strain DS40M4

36.796

98.783

0.363
  pilB Vibrio cholerae strain A1552

36.945

97.913

0.362
  pilB Legionella pneumophila strain ERS1305867

39.245

92.174

0.362
  pilF Thermus thermophilus HB27

37.209

97.217

0.362
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.477

96.522

0.362

Multiple sequence alignment