Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   SA08028_RS00225 Genome accession   NZ_CP045435
Coordinates   52430..53779 (-) Length   449 a.a.
NCBI ID   WP_002486033.1    Uniprot ID   -
Organism   Staphylococcus aureus strain 08-028     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 27532..75160 52430..53779 within 0
SCCmec 44015..58951 52430..53779 within 0


Gene organization within MGE regions


Location: 27532..75160
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SA08028_RS00115 (SA08028_00020) - 28797..29585 (+) 789 WP_001104167.1 two-component system regulatory protein YycI -
  SA08028_RS00120 (SA08028_02283) - 29974..30774 (+) 801 WP_000088649.1 MBL fold metallo-hydrolase -
  SA08028_RS00125 (SA08028_00022) adsA 31002..33320 (+) 2319 WP_000645781.1 LPXTG-anchored adenosine synthase AdsA -
  SA08028_RS00130 (SA08028_02285) rlmH 33688..34167 (+) 480 WP_000704777.1 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH -
  SA08028_RS00135 (SA08028_02287) - 34489..35744 (+) 1256 Protein_24 MrcB family domain-containing protein -
  SA08028_RS00140 (SA08028_02288) - 36159..36398 (+) 240 WP_001282728.1 hypothetical protein -
  SA08028_RS00145 - 36430..37102 (-) 673 Protein_26 IS6 family transposase -
  SA08028_RS00150 (SA08028_03610) - 37188..38171 (-) 984 Protein_27 protein rep -
  SA08028_RS00155 (SA08028_00027) mobV 38416..39678 (-) 1263 WP_000119405.1 MobV family relaxase -
  SA08028_RS00160 (SA08028_02292) bleO 40185..40589 (-) 405 WP_001242578.1 bleomycin binding protein -
  SA08028_RS00165 (SA08028_02293) aadD1 40806..41576 (-) 771 WP_001795128.1 aminoglycoside O-nucleotidyltransferase ANT(4')-Ia -
  SA08028_RS00170 - 41769..42441 (-) 673 Protein_31 IS6 family transposase -
  SA08028_RS00175 (SA08028_00029) - 42701..43444 (+) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  SA08028_RS00180 (SA08028_02294) - 43541..43969 (+) 429 WP_000872606.1 MaoC family dehydratase -
  SA08028_RS00185 (SA08028_00031) mecA 44015..46024 (-) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  SA08028_RS00190 (SA08028_02295) - 46121..47107 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  SA08028_RS00195 (SA08028_02296) - 47094..47339 (+) 246 Protein_36 type I restriction enzyme endonuclease domain-containing protein -
  SA08028_RS00200 (SA08028_00033) - 47330..48940 (-) 1611 Protein_37 IS1182 family transposase -
  SA08028_RS00205 (SA08028_00034) - 48989..49495 (-) 507 WP_001092189.1 DUF1643 domain-containing protein -
  SA08028_RS00210 (SA08028_02298) - 49510..49821 (-) 312 WP_000700851.1 DUF960 family protein -
  SA08028_RS00215 (SA08028_02300) - 49908..50258 (-) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  SA08028_RS00220 (SA08028_00035) ccrB 50780..52408 (-) 1629 WP_001186608.1 cassette chromosome recombinase CcrB Machinery gene
  SA08028_RS00225 (SA08028_00036) ccrA 52430..53779 (-) 1350 WP_002486033.1 cassette chromosome recombinase CcrA Machinery gene
  SA08028_RS00230 (SA08028_00037) cch1 54013..55782 (-) 1770 WP_373684748.1 cassette chromosome replicative helicase -
  SA08028_RS00235 (SA08028_02301) ssb 55807..56103 (-) 297 WP_000273823.1 cassette chromosome ssDNA-binding protein -
  SA08028_RS00240 (SA08028_00038) - 56296..57342 (-) 1047 WP_001042023.1 hypothetical protein -
  SA08028_RS00245 (SA08028_00039) - 57797..58951 (+) 1155 WP_000912116.1 ATP-binding protein -
  SA08028_RS00250 (SA08028_00040) - 58981..59781 (+) 801 WP_000749913.1 abortive infection family protein -
  SA08028_RS00255 (SA08028_03597) - 60081..61336 (+) 1256 Protein_48 MrcB family domain-containing protein -
  SA08028_RS14860 - 61676..62715 (-) 1040 Protein_49 IS3 family transposase -
  SA08028_RS00275 (SA08028_00043) seh 63091..63816 (+) 726 WP_000608674.1 staphylococcal enterotoxin type H -
  SA08028_RS15040 - 64145..64276 (+) 132 WP_256530356.1 hypothetical protein -
  SA08028_RS15045 - 64263..64571 (+) 309 WP_078055703.1 exotoxin beta-grasp domain-containing protein -
  SA08028_RS00285 (SA08028_02304) - 64712..65185 (+) 474 WP_000455974.1 GNAT family N-acetyltransferase -
  SA08028_RS15050 - 65375..65527 (-) 153 Protein_54 dihydrouridine synthase -
  SA08028_RS00295 - 65552..65638 (-) 87 WP_031867549.1 hypothetical protein -
  SA08028_RS00300 (SA08028_00045) - 65736..66491 (-) 756 WP_000355844.1 sulfite exporter TauE/SafE family protein -
  SA08028_RS00305 (SA08028_02306) cstR 66491..66751 (-) 261 WP_001105087.1 persulfide-sensing transcriptional repressor CstR -
  SA08028_RS00310 (SA08028_00046) cstA 66888..67955 (+) 1068 WP_000816129.1 persulfide response sulfurtransferase CstA -
  SA08028_RS00315 (SA08028_00047) cstB 67986..69320 (+) 1335 WP_179976087.1 persulfide dioxygenase-sulfurtransferase CstB -
  SA08028_RS00320 (SA08028_00048) sqr 69339..70532 (+) 1194 WP_001033018.1 type II sulfide:quinone oxidoreductase Sqr -
  SA08028_RS15015 - 70799..71013 (-) 215 Protein_61 universal stress protein -
  SA08028_RS00325 (SA08028_00049) - 71199..72185 (-) 987 WP_000662031.1 tRNA-dihydrouridine synthase -
  SA08028_RS00330 (SA08028_02308) - 72494..72697 (+) 204 WP_000398679.1 DUF6007 family protein -
  SA08028_RS00335 (SA08028_02309) - 72746..73042 (+) 297 WP_000201640.1 TfoX/Sxy family protein -
  SA08028_RS00340 (SA08028_00050) - 73114..74334 (-) 1221 WP_000130763.1 MFS transporter -
  SA08028_RS00345 (SA08028_02310) - 74327..74878 (-) 552 WP_000627024.1 flavodoxin family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52614.32 Da        Isoelectric Point: 9.9100

>NTDB_id=394086 SA08028_RS00225 WP_002486033.1 52430..53779(-) (ccrA) [Staphylococcus aureus strain 08-028]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQLTQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYHVHILSVHDGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYDYH
NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRSQKQTKQTSSDNQLKQKIKCPCCNATLTNMTVRKKNHILRYYVCPKNMNASRFVCDFKGINAQTLEDKVLKV
CQDFYQDQHIYVKIKNAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFRKQMQSLHQQPQRITSISEHQIQHTIQ
NIIQKRFTLNILYPYIETIHIAKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=394086 SA08028_RS00225 WP_002486033.1 52430..53779(-) (ccrA) [Staphylococcus aureus strain 08-028]
ATGAAACAAGTCATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAAGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGACGCAAAGATAATCGTAGTGGGT
ATCGACAATTAACACAATTAATTCAACAAGGACAATGTGACATATTATGCTGTTATCGTCTTAATAGGTTGCATCGTAAT
CTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACGTACCATGTTCATATCTTAAGCGTACATGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGACTCAAGCTTAATATCTTCATCAGTTTAGCCGAACTTGAATCGGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACACATGCACCTTTTGGTTACGATTATCAC
AACGGAACATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGCCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGCCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATCGCAAAAACAAACCAAACAGACATCTTCGGATAATCAACTCAAACAAAAAATCAA
ATGCCCATGTTGTAATGCAACACTTACAAATATGACCGTTAGAAAAAAGAATCATATATTACGTTACTACGTCTGTCCTA
AAAATATGAATGCTTCACGCTTTGTCTGTGATTTCAAAGGCATCAATGCACAGACATTGGAAGATAAAGTATTAAAAGTG
TGCCAAGACTTTTATCAAGACCAACATATCTACGTAAAAATTAAAAATGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAGCATCACACATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATCGATGCAGAAACGTTTA
GAAAACAAATGCAATCATTACATCAACAACCACAACGCATCACATCCATCAGTGAGCATCAAATACAACACACCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTGCGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATCGTCAATCAAACCATGCAATCATCAATTGCATAA

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(169-250)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-322)

Resolvase, N terminal domain (Resolvase)

(4-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus N315

95.546

100

0.955
  ccrA Staphylococcus aureus COL

74.833

100

0.748

Multiple sequence alignment