Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   HIBPF_RS01550 Genome accession   NC_014920
Coordinates   313420..313959 (+) Length   179 a.a.
NCBI ID   WP_005686810.1    Uniprot ID   -
Organism   Haemophilus influenzae F3031     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 308420..318959
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HIBPF_RS01530 (HIBPF03090) - 308881..309801 (-) 921 WP_013525568.1 ATP-binding cassette domain-containing protein -
  HIBPF_RS01535 (HIBPF03100) - 309801..310682 (-) 882 WP_013525569.1 metal ABC transporter substrate-binding protein -
  HIBPF_RS01540 (HIBPF03110) - 311043..311921 (-) 879 WP_013525570.1 D-alanyl-D-alanine carboxypeptidase family protein -
  HIBPF_RS01545 (HIBPF03120) - 312168..313319 (+) 1152 WP_013525571.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  HIBPF_RS01550 (HIBPF03130) pilF/pilF2 313420..313959 (+) 540 WP_005686810.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  HIBPF_RS01555 (HIBPF03140) - 314031..314942 (+) 912 WP_013525572.1 helix-turn-helix domain-containing protein -
  HIBPF_RS01560 (HIBPF03150) ispG 314951..316057 (+) 1107 WP_005654847.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  HIBPF_RS01565 (HIBPF03160) hisS 316067..317338 (+) 1272 WP_013525573.1 histidine--tRNA ligase -
  HIBPF_RS01570 (HIBPF03170) - 317356..317970 (+) 615 WP_013525574.1 YfgM family protein -
  HIBPF_RS01575 (HIBPF03180) iscX 318021..318215 (-) 195 WP_005630102.1 Fe-S cluster assembly protein IscX -
  HIBPF_RS01580 (HIBPF03190) fdx 318215..318556 (-) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20595.25 Da        Isoelectric Point: 8.6216

>NTDB_id=39407 HIBPF_RS01550 WP_005686810.1 313420..313959(+) (pilF/pilF2) [Haemophilus influenzae F3031]
MKTISKQLSAVIFPFIFSACVSQSASNLNHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=39407 HIBPF_RS01550 WP_005686810.1 313420..313959(+) (pilF/pilF2) [Haemophilus influenzae F3031]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTTCCTTTTATTTTTTCTGCCTGCGTTTCACAATCTGCATCTAA
CTTAAATCATCAAGCTGCCGCTAAAGCGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCCCAACAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAGCGGATACATTTGAAAATATTGTGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACT
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

98.883

100

0.989
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447

Multiple sequence alignment