Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   GCU76_RS13450 Genome accession   NZ_CP045425
Coordinates   2477672..2478742 (-) Length   356 a.a.
NCBI ID   WP_017696192.1    Uniprot ID   -
Organism   Bacillus subtilis strain JAAA     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2472672..2483742
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCU76_RS13405 tapA 2473158..2473913 (-) 756 WP_017696199.1 amyloid fiber anchoring/assembly protein TapA -
  GCU76_RS13410 yqzG 2474184..2474510 (+) 327 WP_026113671.1 YqzG/YhdC family protein -
  GCU76_RS13415 spoIITA 2474552..2474731 (-) 180 WP_014480252.1 YqzE family protein -
  GCU76_RS13420 comGG 2474802..2475176 (-) 375 WP_017696197.1 ComG operon protein ComGG Machinery gene
  GCU76_RS13425 comGF 2475177..2475560 (-) 384 WP_017696196.1 ComG operon protein ComGF Machinery gene
  GCU76_RS13430 comGE 2475586..2475933 (-) 348 WP_017696195.1 competence type IV pilus minor pilin ComGE Machinery gene
  GCU76_RS13435 comGD 2475917..2476348 (-) 432 WP_017696194.1 competence type IV pilus minor pilin ComGD Machinery gene
  GCU76_RS13440 comGC 2476338..2476634 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  GCU76_RS13445 comGB 2476648..2477685 (-) 1038 WP_017696193.1 competence type IV pilus assembly protein ComGB Machinery gene
  GCU76_RS13450 comGA 2477672..2478742 (-) 1071 WP_017696192.1 competence protein ComGA Machinery gene
  GCU76_RS13455 - 2478953..2479099 (-) 147 WP_250619918.1 hypothetical protein -
  GCU76_RS13460 corA 2479152..2480105 (-) 954 WP_017696189.1 magnesium transporter CorA -
  GCU76_RS13465 yqhB 2480247..2481575 (+) 1329 WP_017696188.1 hemolysin family protein -
  GCU76_RS13470 rsbRD 2481628..2482464 (-) 837 WP_017696187.1 RsbT co-antagonist protein RsbRD -
  GCU76_RS13480 mgsR 2482688..2483068 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  GCU76_RS13485 yqgY 2483300..2483545 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40472.67 Da        Isoelectric Point: 9.0175

>NTDB_id=393996 GCU76_RS13450 WP_017696192.1 2477672..2478742(-) (comGA) [Bacillus subtilis strain JAAA]
MDSIEKISKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=393996 GCU76_RS13450 WP_017696192.1 2477672..2478742(-) (comGA) [Bacillus subtilis strain JAAA]
TTGGATTCAATAGAAAAGATAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATCGTGCC
GAGGGAGCGAGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGACTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCTCAATACAATATCCCTTCGATTGATAAACTGTCGTTATTTCCAAAGACAGGAGCCACATTACTCTCATTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACTACCACATTATACTCTCTCGTTCAATAC
GCAAAAAAACACTTTAATCGAAATATTGTTACATTAGAGGACCCTGTTGAAACTAGGGACGAAGACGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAGGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGATGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTGACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGGCTGCTTGAATTCGGTATCAATATGAATGAAATTGAACAGACCGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGTCCATTTTGTGAAAACGGATGTTCATCAGTATATTGCCGTCAGTCAC
GAAATACCAGAAGAGCTAGCGTTTATGAGCTTCTTTATGGGAAAAACCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

99.719

100

0.997

Multiple sequence alignment