Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HP431_RS09405 Genome accession   NZ_CP053731
Coordinates   1936936..1937376 (+) Length   146 a.a.
NCBI ID   WP_000360902.1    Uniprot ID   A7ZHJ7
Organism   Escherichia coli strain CP55_Sichuan     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1931936..1942376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HP431_RS09385 (HP431_09385) aroP 1932934..1934307 (+) 1374 WP_000969914.1 aromatic amino acid transporter AroP -
  HP431_RS09390 (HP431_09390) ampE 1934350..1935204 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  HP431_RS09395 (HP431_09395) ampD 1935201..1935752 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HP431_RS09400 (HP431_09400) nadC 1935840..1936733 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HP431_RS09405 (HP431_09405) pilA 1936936..1937376 (+) 441 WP_000360902.1 prepilin peptidase-dependent pilin Machinery gene
  HP431_RS09410 (HP431_09410) pilB 1937386..1938771 (+) 1386 WP_001025155.1 type II secretion system protein GspE Machinery gene
  HP431_RS09415 (HP431_09415) hofC 1938761..1939963 (+) 1203 WP_000157234.1 protein transport protein HofC -
  HP431_RS09420 (HP431_09420) guaC 1939998..1941041 (-) 1044 WP_001217338.1 GMP reductase -
  HP431_RS26520 - 1941197..1941241 (-) 45 WP_120795372.1 protein YacM -
  HP431_RS09425 (HP431_09425) coaE 1941266..1941886 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15630.76 Da        Isoelectric Point: 4.5559

>NTDB_id=393872 HP431_RS09405 WP_000360902.1 1936936..1937376(+) (pilA) [Escherichia coli strain CP55_Sichuan]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=393872 HP431_RS09405 WP_000360902.1 1936936..1937376(+) (pilA) [Escherichia coli strain CP55_Sichuan]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZHJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

41.791

91.781

0.384
  pilA Haemophilus influenzae Rd KW20

41.045

91.781

0.377