Detailed information NTG0393791

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: FIU87_RS06395
• Genome accession: NZ_CP045403
• Coordinates: 1210034..1213804 (+)
• Length: 1256 a.a.
• NCBI ID: WP_152443809.1
• Uniprot ID: A0A5P9HLW0
• Organism: Bacillus sp. THAF10
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1205034..1218804

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FIU87_RS06390 (FIU87_06605)	addB	1206522..1210037 (+)	3516	WP_152443808.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
FIU87_RS06395 (FIU87_06610)	addA	1210034..1213804 (+)	3771	WP_152443809.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
FIU87_RS06400 (FIU87_06615)	sbcD	1213821..1214999 (+)	1179	WP_152443810.1	exonuclease subunit SbcD	-
FIU87_RS06405 (FIU87_06620)	-	1214996..1218415 (+)	3420	WP_152443811.1	AAA family ATPase	-
FIU87_RS06410 (FIU87_06625)	-	1218466..1218684 (-)	219	WP_152443812.1	spore germination protein	-

Sequence

Protein

• Length: 1256 a.a.
• Molecular weight: 145523.25 Da
• Isoelectric Point: 4.9629

>NTDB_id=393791 FIU87_RS06395 WP_152443809.1 1210034..1213804(+) (addA) [Bacillus sp. THAF10]
MILDVIPKPDGAQWTDDQWKAIVASGRDMLVAAAAGSGKTAVLVERIITKMVSEHVDVDRLLVVTFTNASAAEMRHRIGE
ALEKELEKKPASLHLRRQLSLLNRASISTIHSFCLEVIRKYYYLIDIDPGFRIADTTEIQLLQEEVLEMVFEEEYGQEND
AFFDLVDRFTSDRTDHALQELVLKLYEFSRANEDPEQWLEDIVTFYRSANEESIDHFPFIEMLKNMIKLQLEAARTLLEK
ASELTLRPGGPAPRAENVEQDLSQLFRLLEGLNLSWEDLYASMQELEFPRAKTCRGDEYDKDLLEAWTKARNQAKDIVNG
IKEEFFTRRPESLLTDLAELAPVMDMLVKLVKKYGNAFHLLKQEKGLVDFSDLEHLCLRILRKKTGDGWTRSEAALRYQS
TFKEVMVDEYQDTNMVQEAILKLVTKDSEEEGNMFMVGDVKQSIYRFRLAEPFLFLSKYKRFHSEGYETGLKIDLNQNFR
SRPEILDATNFLFKQMMGETVGEIDYNDDAELKFGASYYPEASNRETELLLIDRSDSLLNEEDDDQPSDGAETNTAFDKT
ELETAQVEAKLIAKRIKQLIQNGYEVYDKHLKVMRPVTYRDFVILMRSMPWAPQFMDEFKQQGLPLYANLSTGYFEATEV
AILLSLLKIIDNPYQDVPLAAVLRSPIVGLDSNDLATIRIQQKKGTYFEALLAFLERQPDSVEERELLYKVKEFYELLQA
WRTQAREGALSELIWKVYQDTHFYDFVGGMPGGKQRQANLRALYDRARQYEATSFRGLFRFLRFIERMQDRGEDLGAARA
LGEQEDVVRLMTIHSSKGLEFPIVFVAGLSKQFNMMDLRKSYLMDKELGFGASFVNPKLRITYPTILQLAMKHKAKAQLI
AEEMRVLYVALTRAKEKLYLVGTLKDAEKSMQNWNESVTHSDWLLPDYVRANAKSYMDWIGPALIRHNDTELLRDKEQTT
ECAAEITAHPSKWKVTMTNVHELLEEIEEDKAVEDEQINRLRSGLPVSMQSPLKAQVESQLYWNYPYTKASLQRSKQSVS
EIKRLREQQDPYAEQTMIRKEKRFFLDRPAFLQKKQLSPTEVGTAMHAVMQNIKLSLEEEMNRDHLQAQVQEMVQKELLS
KEQAEVIDFEAILSFFHTKIGRKMLHAKQIHREIPFSYALQGDDMMEKPGVSEETVLIQGVIDCLIEDEEGIILIDYKTD
KINERYSSITEAEPILKRRYQEQVALYGKAVEHIWKKQLSAKYLYFFDGNHLIQMD

Nucleotide

• Length: 3771 bp

>NTDB_id=393791 FIU87_RS06395 WP_152443809.1 1210034..1213804(+) (addA) [Bacillus sp. THAF10]
GTGATATTAGACGTTATTCCAAAGCCAGATGGGGCACAATGGACAGACGATCAATGGAAGGCAATAGTAGCTTCTGGCAG
AGATATGCTTGTTGCCGCAGCAGCTGGTTCCGGGAAAACAGCCGTGTTAGTCGAGCGGATTATTACTAAAATGGTAAGTG
AGCATGTGGATGTAGACAGACTTCTTGTTGTAACGTTCACCAATGCATCAGCAGCCGAAATGAGGCACAGAATTGGAGAA
GCACTAGAAAAAGAGCTTGAGAAAAAACCAGCCTCTTTGCACTTGCGCAGGCAGCTTAGTTTATTAAATCGTGCATCCAT
CTCTACCATCCATTCCTTTTGTCTGGAGGTAATTAGAAAATATTATTACTTAATTGATATCGATCCAGGCTTTCGTATTG
CAGATACAACGGAAATTCAATTGCTGCAGGAAGAAGTCTTAGAAATGGTATTTGAAGAGGAGTATGGACAAGAGAACGAT
GCTTTCTTTGACCTTGTTGACCGTTTTACAAGCGACCGAACAGACCATGCCTTACAGGAGTTAGTCCTCAAACTTTATGA
ATTTTCTCGTGCAAACGAAGACCCAGAGCAGTGGCTTGAGGATATTGTGACGTTTTATCGGTCAGCAAATGAGGAGAGCA
TCGATCATTTTCCCTTTATAGAAATGCTGAAAAATATGATAAAGCTTCAACTTGAAGCTGCTCGTACTCTTTTGGAAAAG
GCGAGTGAGCTTACGCTTCGCCCAGGAGGACCTGCTCCGCGTGCAGAAAATGTAGAACAAGATCTTTCTCAGCTATTCCG
GCTGCTGGAAGGGCTAAATCTATCATGGGAGGATTTATACGCTTCCATGCAGGAGTTAGAGTTTCCTCGAGCAAAAACCT
GCCGTGGCGATGAATATGACAAAGATTTGCTGGAAGCGTGGACAAAGGCTCGTAATCAAGCAAAAGACATCGTGAATGGA
ATAAAGGAAGAATTTTTCACTAGAAGGCCAGAATCCTTGTTAACGGACTTGGCGGAGCTTGCCCCTGTTATGGACATGTT
AGTGAAATTGGTGAAAAAATATGGAAACGCCTTTCATTTACTAAAGCAGGAAAAAGGATTGGTCGATTTTTCTGATTTGG
AGCATCTATGCTTACGTATTCTTAGGAAGAAAACGGGAGACGGCTGGACCCGCTCTGAAGCGGCCCTTCGATATCAGTCT
ACCTTTAAAGAAGTCATGGTCGATGAATACCAGGATACGAATATGGTGCAAGAAGCAATATTAAAGCTAGTTACAAAGGA
TTCAGAAGAGGAAGGAAACATGTTTATGGTTGGGGATGTGAAGCAGTCCATCTATCGTTTCCGATTAGCAGAACCATTCC
TTTTCTTAAGCAAGTATAAAAGGTTTCATTCAGAGGGTTATGAAACAGGATTAAAAATTGATCTAAATCAAAATTTCCGC
TCTCGTCCTGAAATTCTTGATGCGACGAACTTCCTTTTTAAACAAATGATGGGTGAAACAGTTGGGGAAATTGACTATAA
CGACGATGCGGAGCTTAAATTTGGAGCCTCCTATTACCCTGAGGCCAGCAACAGAGAAACAGAGCTTCTTTTAATTGATA
GAAGTGACTCTCTTTTGAATGAAGAAGATGATGATCAACCAAGTGATGGGGCAGAGACAAACACAGCTTTTGATAAAACG
GAGCTTGAAACAGCACAAGTGGAAGCAAAACTCATCGCAAAAAGGATCAAACAGCTTATTCAAAATGGCTATGAGGTGTA
TGACAAGCATCTGAAAGTGATGCGGCCTGTCACTTATCGAGATTTTGTTATTTTAATGCGATCGATGCCATGGGCGCCAC
AATTTATGGATGAGTTCAAACAACAGGGTCTTCCCCTTTACGCAAATCTAAGCACAGGTTATTTTGAAGCAACGGAAGTG
GCCATACTCTTGTCGTTGCTAAAAATAATTGATAACCCATATCAGGATGTTCCGCTTGCTGCCGTTTTACGTTCTCCTAT
TGTAGGGCTTGATTCCAATGATCTCGCTACGATAAGAATACAACAAAAAAAGGGCACCTATTTTGAAGCACTTCTGGCCT
TTTTAGAGCGACAGCCTGATTCAGTAGAAGAGAGAGAACTCCTTTATAAGGTAAAAGAGTTTTATGAGTTGCTTCAGGCT
TGGAGAACTCAAGCTAGAGAAGGAGCCTTATCAGAGCTGATCTGGAAAGTCTATCAAGATACACACTTTTATGATTTTGT
TGGAGGCATGCCTGGAGGAAAACAGCGCCAGGCCAACCTACGTGCATTATATGACCGAGCAAGACAATATGAAGCTACCT
CATTTCGAGGACTTTTCCGCTTTTTACGCTTCATTGAAAGAATGCAAGACCGCGGGGAGGACCTTGGAGCAGCAAGAGCC
CTTGGAGAACAGGAAGATGTGGTTCGTTTAATGACGATTCACTCAAGCAAGGGGTTAGAGTTTCCAATCGTTTTTGTAGC
TGGTTTATCCAAGCAATTTAATATGATGGATTTGAGAAAAAGCTACTTAATGGATAAAGAGCTTGGCTTTGGTGCTAGCT
TTGTCAACCCTAAGCTGAGAATTACGTATCCGACTATTTTACAGCTCGCCATGAAGCATAAAGCAAAAGCACAGCTGATT
GCAGAAGAAATGAGGGTGCTTTATGTGGCGCTGACTCGTGCAAAAGAAAAGCTTTACCTAGTCGGTACTTTAAAGGACGC
AGAAAAATCAATGCAAAATTGGAATGAATCTGTTACGCATTCAGACTGGCTTCTACCAGATTACGTAAGAGCAAATGCAA
AGTCCTATATGGACTGGATTGGTCCAGCTCTTATTCGCCATAACGATACAGAGTTACTTCGTGATAAAGAGCAAACAACA
GAGTGTGCTGCTGAAATCACTGCTCATCCTTCAAAATGGAAGGTTACGATGACCAACGTCCACGAGCTTCTAGAAGAGAT
AGAAGAGGATAAAGCAGTGGAAGACGAACAAATTAATAGGCTGAGAAGTGGGTTACCAGTATCGATGCAAAGTCCTCTAA
AAGCTCAGGTTGAGTCCCAACTATACTGGAATTACCCTTATACAAAAGCGAGTCTTCAACGATCAAAACAGTCAGTGTCG
GAAATAAAAAGGTTGAGAGAGCAACAAGATCCTTATGCAGAACAGACGATGATTCGTAAGGAAAAAAGATTCTTCCTTGA
CAGACCCGCCTTTTTACAAAAGAAACAGCTATCACCTACAGAGGTGGGGACCGCTATGCATGCTGTCATGCAAAATATCA
AGCTGAGCTTGGAAGAGGAGATGAACAGGGACCATCTGCAGGCACAGGTCCAAGAGATGGTGCAAAAAGAGTTACTCTCA
AAAGAACAAGCTGAAGTCATCGATTTTGAAGCAATCCTGTCATTCTTCCACACAAAGATTGGGAGAAAAATGCTTCATGC
TAAACAAATTCATAGAGAAATTCCGTTCAGCTATGCCCTTCAAGGCGATGACATGATGGAAAAGCCTGGAGTTTCGGAGG
AAACCGTTTTAATCCAAGGTGTTATTGACTGTTTGATAGAGGATGAAGAAGGGATCATTTTGATTGATTACAAGACAGAT
AAAATTAATGAACGCTATTCAAGTATTACAGAAGCAGAACCAATTTTAAAAAGAAGATATCAAGAACAGGTTGCCCTGTA
TGGAAAAGCAGTAGAACATATTTGGAAAAAGCAGCTTTCTGCCAAGTATTTATATTTCTTTGATGGAAATCACCTGATTC
AAATGGATTAA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (473-901)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1063-1247)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-463)

3D structure

• AlphaFold DB: A0A5P9HLW0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	55.096	100	0.551