Detailed information NTG0393790

Bioinformatically predicted

Overview

• Name: addB
• Type: Machinery gene
• Locus tag: FIU87_RS06390
• Genome accession: NZ_CP045403
• Coordinates: 1206522..1210037 (+)
• Length: 1171 a.a.
• NCBI ID: WP_152443808.1
• Uniprot ID: A0A5P9HLY5
• Organism: Bacillus sp. THAF10
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1201522..1215037

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FIU87_RS06380 (FIU87_06595)	-	1201891..1204947 (+)	3057	WP_152443806.1	efflux RND transporter permease subunit	-
FIU87_RS06385 (FIU87_06600)	-	1204978..1206123 (-)	1146	WP_253905574.1	MFS transporter	-
FIU87_RS06390 (FIU87_06605)	addB	1206522..1210037 (+)	3516	WP_152443808.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
FIU87_RS06395 (FIU87_06610)	addA	1210034..1213804 (+)	3771	WP_152443809.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
FIU87_RS06400 (FIU87_06615)	sbcD	1213821..1214999 (+)	1179	WP_152443810.1	exonuclease subunit SbcD	-

Sequence

Protein

• Length: 1171 a.a.
• Molecular weight: 135198.91 Da
• Isoelectric Point: 6.2657

>NTDB_id=393790 FIU87_RS06390 WP_152443808.1 1206522..1210037(+) (addB) [Bacillus sp. THAF10]
MSVQFLIGRTGSGKTHWCHEHILHKLKEDPQGTPIVYLVPEQMTFQSEYRLVQSPEIKGMIRSQVFSFTRLAWRILQEVG
GISRFHIDQTGIHMLLRKVVEEHKQEFHVFGRSAEQFGFIEEMEGMITEFKRYCITPETLEADITKMRQDASITEHEKML
AKKLTDILVVYRQLEQALEGKYLDGEDYLALLAEKIPESSYIQKADIYIDGFHSFTPQELNVLKELMKHANSVTIALTLD
KPYDEEAPYDLHLFRSTGMTYQRLRSLALEEACEMKEPIVLEEASRFSTSHSLHHLEKFFDVRPTVSYENTTDVTILQAV
NRRAEVEGIAREIISLVRDKGNRYRDIAIIIRNSEAYADLFNTVFQDFQIPFFIDQKRTMLHHPLVELLRSSMEVITKNW
RYESVFRCVKTDFFYSTDQLVQEMREKTDKLENYCLSYGIQGKKWTSEIPWTYRRYFSVDTDGPYIQKEDEIAYEQELNE
LRKMIVQPLHTLEKRWKKAKTIEDYCKALYEYLEELQIPQKLEARMRKSEASGNLAAAREHEQVWKAIIQMLDQMVELLK
GQPMSTSMFTKMLESGLESLRFSLVPPSMDQVIVANYDLSRLTDIKHTFVIGVNDAVIPAKVKDEGFVSEEERELLANYG
MELAPSSMEKLLDENFLAYTAFLTPSQHLYISYSLADEEGKSLLASSYIKRIKEMFPRIEEQLLLNDPTENKQEHPLRYI
HTPATTLSNLAIQLQGWMKKYPVEPTWWDVYNTLLENQKWSERTKHVVNSLFYKNEAKKLTNSISRELYGTHMEASVSRM
EKFSACAFSHFASHGLKLKDRKIYRLEAPDIGELFHAALKHISDVLRHNKTDWKSLTKQQCEVLAREAVDMLSPKLQGEI
LLSSNRFQYIKKKLETVITRASQILSDHAKASKFTPVGLELGFGRTGELPPIKIDLPNDVTMELIGRIDRVDRAEGSNGL
LLRIVDYKSSKKALDLTEVYYGLALQMLTYLDIVITHSKSWLGDQANPAGVLYFHVHNPMIKKSGMIPEDIIEEEIFKSF
KMKGLLLGDEETVRLMDQTVDSGHSKIVSASFKKNGGFQAASSIASEEEFTSLRKYVRQTFEEIGSKIAEGETAIAPYKL
KDQVPCTFCSYKPFCQFDTSLDENDYRVLQSIPKDEVITSIKDKLEGGGTR

Nucleotide

• Length: 3516 bp

>NTDB_id=393790 FIU87_RS06390 WP_152443808.1 1206522..1210037(+) (addB) [Bacillus sp. THAF10]
ATGTCTGTGCAATTTTTAATAGGGAGAACAGGAAGTGGAAAGACCCATTGGTGTCATGAGCACATCCTTCATAAACTGAA
GGAAGACCCTCAAGGAACTCCAATTGTTTACCTCGTTCCTGAACAAATGACCTTTCAATCAGAATATCGACTTGTACAAT
CACCTGAAATTAAAGGGATGATTCGTTCGCAAGTATTTAGCTTTACAAGACTAGCATGGAGGATTCTGCAAGAGGTCGGG
GGAATCAGCCGTTTTCATATTGACCAAACAGGGATACATATGCTGCTTAGAAAAGTGGTAGAAGAGCATAAGCAAGAGTT
TCATGTTTTCGGTAGATCTGCAGAACAATTTGGTTTTATTGAGGAAATGGAAGGCATGATAACGGAATTCAAACGCTATT
GCATTACACCGGAAACACTAGAAGCGGACATCACAAAAATGAGACAGGATGCTTCGATTACTGAGCACGAGAAAATGCTT
GCTAAAAAGCTAACCGATATTCTTGTAGTTTATCGTCAGCTTGAACAAGCCTTAGAAGGAAAGTATCTTGATGGAGAGGA
TTACTTGGCATTGCTTGCCGAAAAAATTCCCGAGTCCTCCTACATTCAAAAAGCTGATATTTATATTGACGGCTTTCATA
GCTTTACACCACAGGAATTAAATGTCCTAAAAGAATTAATGAAACATGCTAATTCTGTCACGATTGCATTAACGTTAGAT
AAACCATATGACGAAGAGGCGCCATATGATTTACATCTGTTCCGCTCCACAGGCATGACGTATCAAAGGCTAAGAAGTCT
TGCGTTAGAAGAAGCTTGTGAAATGAAAGAGCCAATTGTTTTAGAGGAAGCTAGTAGATTTTCAACTTCTCATTCCCTTC
ATCATCTGGAGAAATTTTTTGATGTTCGGCCAACAGTTAGCTATGAAAATACAACGGACGTTACGATACTACAAGCTGTA
AACAGAAGAGCAGAAGTGGAAGGGATTGCTAGAGAGATTATTTCACTCGTTAGGGATAAGGGTAACCGTTATAGGGATAT
TGCGATTATTATACGTAACAGTGAAGCCTATGCGGACCTTTTTAATACAGTGTTTCAGGATTTTCAGATACCTTTCTTTA
TTGACCAGAAACGAACGATGCTTCACCATCCTTTAGTCGAGCTGCTGAGATCCAGTATGGAGGTTATTACGAAAAATTGG
CGCTATGAATCTGTGTTTCGTTGCGTGAAAACGGACTTCTTTTACTCTACCGATCAACTTGTTCAAGAGATGAGAGAGAA
AACGGATAAGCTAGAAAATTATTGTCTTTCCTATGGAATACAAGGCAAGAAATGGACAAGTGAAATTCCATGGACATATC
GCCGTTATTTCAGTGTAGATACAGATGGTCCTTACATTCAAAAAGAAGATGAAATTGCATACGAACAAGAATTAAATGAA
CTGCGAAAAATGATCGTTCAGCCACTTCATACACTAGAAAAACGTTGGAAAAAAGCGAAGACAATCGAGGACTATTGTAA
AGCACTATATGAATATTTAGAGGAATTGCAAATCCCGCAAAAGCTGGAAGCGAGGATGAGGAAAAGCGAAGCATCTGGAA
ATTTAGCAGCTGCAAGGGAACATGAACAGGTATGGAAAGCGATTATTCAGATGCTCGATCAAATGGTGGAGCTTCTAAAA
GGCCAGCCAATGAGTACCTCCATGTTTACAAAGATGCTTGAAAGTGGATTAGAAAGTCTTCGCTTCAGTCTTGTCCCTCC
ATCAATGGATCAGGTTATTGTGGCAAACTACGATCTTTCCCGGTTAACAGACATAAAGCATACCTTTGTGATTGGTGTAA
ATGACGCTGTTATCCCTGCAAAAGTAAAGGATGAAGGGTTTGTTTCAGAAGAGGAACGGGAGCTGCTCGCAAACTATGGG
ATGGAGCTAGCACCTTCTAGCATGGAAAAACTTCTTGACGAGAATTTTTTAGCATATACAGCATTTTTAACACCATCCCA
GCACCTGTATATAAGCTATAGTTTAGCCGATGAAGAAGGAAAATCCTTATTAGCCTCCTCCTATATAAAACGGATAAAAG
AGATGTTTCCTAGAATAGAAGAGCAACTTCTGTTAAATGATCCAACTGAAAATAAACAGGAACATCCATTACGGTACATT
CACACCCCAGCGACAACTCTCTCCAACCTTGCCATACAGCTTCAAGGATGGATGAAGAAATATCCTGTGGAACCTACTTG
GTGGGATGTCTATAATACGCTACTTGAAAATCAAAAATGGTCAGAGCGAACCAAGCATGTCGTGAATAGCTTGTTTTATA
AAAATGAAGCCAAAAAATTAACAAACTCTATCAGCAGAGAGCTTTATGGTACACACATGGAGGCAAGTGTATCGAGGATG
GAAAAGTTCAGTGCATGCGCATTTTCCCATTTTGCTTCTCATGGTCTCAAATTAAAGGACAGGAAAATCTATCGTTTGGA
AGCACCCGATATTGGAGAGCTTTTTCACGCGGCCTTAAAGCATATTTCAGATGTTCTAAGACACAACAAAACGGATTGGA
AAAGTCTCACGAAGCAGCAATGTGAAGTACTGGCAAGGGAAGCGGTGGATATGCTTTCTCCTAAACTGCAAGGGGAGATT
CTGCTTAGCTCCAACCGGTTCCAATACATTAAAAAGAAGCTTGAAACAGTCATTACGAGAGCATCACAAATCCTAAGCGA
TCACGCAAAAGCGAGTAAATTTACACCGGTAGGACTCGAGCTTGGGTTTGGAAGAACGGGCGAGCTTCCGCCAATAAAAA
TTGACTTACCAAATGATGTAACGATGGAGCTGATTGGCAGAATTGATAGAGTCGACCGTGCGGAAGGAAGTAACGGCTTG
CTCCTACGAATTGTTGACTACAAATCTAGCAAAAAGGCACTCGATCTTACGGAAGTGTACTACGGTTTAGCCCTACAAAT
GCTTACCTATTTGGATATCGTCATCACGCATTCCAAAAGCTGGTTAGGTGATCAGGCGAACCCAGCAGGTGTCTTGTATT
TTCATGTGCATAATCCAATGATTAAAAAGAGTGGAATGATACCAGAGGATATCATTGAAGAAGAAATTTTTAAAAGCTTT
AAAATGAAAGGCTTGCTGTTAGGCGATGAGGAGACCGTTCGATTAATGGACCAAACGGTAGATAGCGGTCACTCCAAAAT
TGTCTCAGCCTCCTTCAAAAAGAACGGTGGCTTTCAAGCTGCCTCCTCCATCGCAAGTGAAGAGGAGTTTACGAGTCTTA
GAAAATACGTAAGGCAAACCTTTGAGGAGATTGGAAGTAAAATAGCAGAAGGAGAAACAGCCATTGCACCGTATAAACTA
AAAGATCAGGTTCCATGTACATTTTGTTCCTATAAGCCTTTTTGTCAATTTGACACATCACTTGATGAGAATGATTACAG
AGTTCTGCAGAGTATTCCGAAAGATGAGGTTATCACCTCCATTAAAGATAAGTTGGAAGGAGGAGGTACAAGGTGA

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (795-1136)

• UvrD-like helicase C-terminal domain (UvrD_C) (296-560)

3D structure

• AlphaFold DB: A0A5P9HLY5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addB	Bacillus subtilis subsp. subtilis str. 168	51.066	100	0.512