Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   HP432_RS02390 Genome accession   NZ_CP053730
Coordinates   278383..279060 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain CP61_Sichuan     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 273383..284060
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HP432_RS02355 (HP432_02360) gspG 273863..274300 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  HP432_RS02360 (HP432_02365) gspH 274308..274817 (+) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  HP432_RS02365 (HP432_02370) gspI 274814..275191 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  HP432_RS02370 (HP432_02375) gspJ 275184..275771 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  HP432_RS02375 (HP432_02380) gspK 275764..276747 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  HP432_RS02380 (HP432_02385) gspL 276762..277925 (+) 1164 WP_001115238.1 type II secretion system protein GspL -
  HP432_RS02385 (HP432_02390) gspM 277922..278383 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  HP432_RS02390 (HP432_02395) pilD 278383..279060 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  HP432_RS02395 (HP432_02400) bfr 279089..279565 (-) 477 WP_000675504.1 bacterioferritin -
  HP432_RS02400 (HP432_02405) bfd 279637..279831 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  HP432_RS02405 (HP432_02410) chiA 280000..282693 (-) 2694 WP_094322432.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=393780 HP432_RS02390 WP_000178161.1 278383..279060(+) (pilD) [Escherichia coli strain CP61_Sichuan]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=393780 HP432_RS02390 WP_000178161.1 278383..279060(+) (pilD) [Escherichia coli strain CP61_Sichuan]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment