Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   FIU98_RS02735 Genome accession   NZ_CP045359
Coordinates   581599..583707 (+) Length   702 a.a.
NCBI ID   WP_152487719.1    Uniprot ID   -
Organism   Pseudomonas sp. THAF42     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 576599..588707
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU98_RS02715 (FIU98_02690) comM 578766..579830 (+) 1065 WP_152487715.1 pilus assembly protein PilM Machinery gene
  FIU98_RS02720 (FIU98_02695) pilN 579830..580402 (+) 573 WP_152487716.1 type 4a pilus biogenesis protein PilN -
  FIU98_RS02725 (FIU98_02700) pilO 580399..581019 (+) 621 WP_152487717.1 type 4a pilus biogenesis protein PilO -
  FIU98_RS02730 (FIU98_02705) pilP 581019..581543 (+) 525 WP_152487718.1 type 4a pilus biogenesis lipoprotein PilP -
  FIU98_RS02735 (FIU98_02710) pilQ 581599..583707 (+) 2109 WP_152487719.1 type IV pilus secretin PilQ Machinery gene
  FIU98_RS02740 (FIU98_02715) aroK 583713..584231 (+) 519 WP_152487720.1 shikimate kinase AroK -
  FIU98_RS02745 (FIU98_02720) aroB 584283..585386 (+) 1104 WP_152487721.1 3-dehydroquinate synthase -
  FIU98_RS02750 (FIU98_02725) - 585397..586968 (+) 1572 WP_152487722.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 702 a.a.        Molecular weight: 75893.06 Da        Isoelectric Point: 4.7096

>NTDB_id=393631 FIU98_RS02735 WP_152487719.1 581599..583707(+) (pilQ) [Pseudomonas sp. THAF42]
MNGSLSRLSVAFMAALLSPVVLAANLQGLDVASLPGDRVELKLSFDEPVTAPRGYTIEQPARIALDLPGVSNKLGSKNRE
LGIGNARSVTIVEAKDRTRLIVNLTSLAAYSTRVEGNDLFVVVGDSAVAPRPATTPVAATPAPAKKAYGPQAKAISSIDF
QRGEQGEGNVVITLSDKSVAPDIQEQGGKIRLDFAKTELPESLRVRLDVKDFATPVQFVSATGSSNKASIVIEPVGFYDY
LAYQTENKLTLSVKPLTQEDVERRNADRFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVAGNITLRLQNVPWD
QALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELIQVNYAKATDMAKLFQSVTSADGAADD
RGSITVDDRTNSIIAYQTQERLDELRRIIAQLDIPVRQVMIEARIVEANVDYNKALGVRWGGASRRGNWSVYGKDGATSF
DDDGRVLLPGTDTVGDFTLDEGTAPVPFVDMGVANSTAGIGIGFLTDNITLDLQLSAMEATGNGEVISQPKVVTSDKETA
KILKGQEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPNFQAAINGVPPINKNEVNAKVLVNDGE
TIVIGGVFENTQTKATEKVPFLGDLPFIGRVFRRDIVQDEKTELLVFITPRIMNNNSIAVNR

Nucleotide


Download         Length: 2109 bp        

>NTDB_id=393631 FIU98_RS02735 WP_152487719.1 581599..583707(+) (pilQ) [Pseudomonas sp. THAF42]
ATGAACGGCTCTCTTTCGCGTCTTAGTGTCGCCTTCATGGCAGCGCTGCTGTCGCCTGTAGTCCTGGCGGCCAATCTGCA
AGGCCTGGATGTCGCCAGCCTGCCTGGTGACCGGGTCGAGCTGAAACTGTCTTTCGATGAGCCGGTGACGGCGCCGCGAG
GCTACACCATCGAGCAGCCCGCTCGCATCGCCCTGGATCTTCCGGGTGTATCGAACAAACTTGGTTCCAAGAACCGGGAA
CTGGGTATCGGTAATGCGCGCAGCGTCACCATTGTCGAGGCCAAGGACCGCACGCGCCTGATCGTCAATCTGACAAGCCT
GGCGGCCTATAGCACGCGTGTCGAAGGCAACGATCTGTTCGTCGTTGTCGGTGACTCTGCTGTCGCTCCTCGTCCGGCTA
CTACGCCTGTCGCAGCAACTCCCGCTCCTGCCAAGAAGGCCTATGGTCCGCAGGCGAAGGCAATCAGTAGTATCGACTTC
CAGCGTGGCGAGCAGGGTGAAGGTAACGTCGTCATTACCCTTTCCGACAAGTCCGTCGCACCTGATATTCAGGAGCAGGG
CGGCAAGATTCGTCTCGACTTCGCCAAGACCGAGTTGCCTGAGTCCTTGCGTGTACGTCTGGACGTGAAGGATTTCGCGA
CTCCAGTTCAGTTCGTCAGTGCCACCGGTAGTTCCAACAAGGCGAGCATCGTCATCGAGCCAGTAGGTTTTTACGATTAC
CTCGCCTATCAGACCGAGAACAAGCTGACCCTCAGCGTCAAGCCACTGACTCAGGAGGATGTCGAGCGTCGCAATGCGGA
TCGTTTTGCCTACACGGGTGAGAAGCTGTCGCTGAACTTCCAGGACATCGATGTCCGCTCCGTGCTGCAGCTGATTGCGG
ACTTCACCGATCTGAACCTGGTGGCCAGTGATACCGTCGCCGGTAATATCACCCTGCGTCTGCAGAACGTGCCATGGGAT
CAGGCGCTGGACCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGTGGGCAACGTGCTGTTGGTCGCACCGGCCGA
CGAGATCGCCGCCCGCGAGCGCCAGGAGCTGGAGTCGCAGAAGCAGATCGCCGAACTCGCTCCGCTGCGTCGTGAGCTGA
TTCAGGTCAACTACGCCAAGGCGACCGATATGGCCAAGCTGTTCCAGTCGGTGACCAGTGCCGATGGTGCGGCTGATGAC
CGTGGTTCTATCACGGTGGACGATCGCACCAATAGCATCATTGCTTATCAGACCCAGGAACGTCTGGACGAGCTGCGCCG
GATCATTGCCCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGAATCGTGGAGGCCAACGTTGACTACAACA
AGGCGTTGGGTGTTCGCTGGGGTGGTGCTTCCCGCCGTGGTAACTGGAGTGTGTATGGCAAGGATGGTGCTACGTCATTT
GATGATGACGGGCGGGTTTTGCTGCCGGGTACCGATACTGTCGGCGACTTCACGCTGGATGAGGGTACCGCTCCTGTGCC
GTTCGTTGACATGGGGGTCGCCAATAGTACAGCGGGCATCGGCATTGGCTTCCTGACTGACAACATCACATTGGACCTGC
AGCTTTCTGCCATGGAAGCGACCGGTAACGGTGAGGTCATCTCCCAGCCCAAGGTCGTGACTTCCGACAAGGAGACCGCG
AAGATTCTCAAAGGGCAGGAAGTGCCTTACCAGGAGGCCAGTTCCAGTGGTGCGACCAGCACCTCCTTCAAAGAGGCCGC
TCTATCGCTTGAGGTGACGCCGCAGATCACGCCGGATAACCGCATCATCATGGAAGTCAAGGTGACCAAGGACGCCCCGA
ACTTCCAGGCCGCCATCAACGGCGTTCCGCCGATCAACAAGAACGAGGTCAATGCCAAGGTGCTGGTCAACGATGGTGAA
ACCATCGTGATCGGTGGGGTATTCGAGAATACCCAGACCAAGGCGACCGAGAAGGTGCCTTTCCTCGGTGACCTGCCGTT
CATCGGTCGCGTGTTCCGTCGCGACATCGTGCAGGACGAAAAGACCGAGTTGCTGGTTTTCATCACTCCGCGCATCATGA
ATAACAATTCTATTGCCGTGAATCGATGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-121)

Bacterial type II and III secretion system protein (Secretin)

(537-693)

Secretin and TonB N terminus short domain (STN)

(299-345)

Bacterial type II/III secretion system short domain (Secretin_N)

(373-438)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

76.19

100

0.775
  comQ Acinetobacter baylyi ADP1

43.434

98.718

0.429
  pilQ Acinetobacter baumannii strain A118

40.108

100

0.422
  pilQ Acinetobacter baumannii D1279779

40.108

100

0.422
  pilQ Legionella pneumophila strain ERS1305867

38.872

96.011

0.373
  pilQ Legionella pneumophila strain Lp02

38.724

96.011

0.372

Multiple sequence alignment