Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   GCU53_RS07695 Genome accession   NZ_CP045302
Coordinates   1627982..1629574 (+) Length   530 a.a.
NCBI ID   WP_152389817.1    Uniprot ID   -
Organism   Azotobacter salinestris strain KACC 13899     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 1622982..1634574
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCU53_RS07675 rluD 1623644..1624615 (-) 972 WP_152387098.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  GCU53_RS07680 - 1624762..1625775 (+) 1014 WP_152387099.1 outer membrane protein assembly factor BamD -
  GCU53_RS07685 - 1625873..1627507 (-) 1635 WP_208845454.1 NAD+ synthase -
  GCU53_RS07690 - 1627735..1627977 (+) 243 WP_152387101.1 PP0621 family protein -
  GCU53_RS07695 pilS 1627982..1629574 (+) 1593 WP_152389817.1 ATP-binding protein Regulator
  GCU53_RS07700 pilR 1629636..1630976 (+) 1341 WP_152387102.1 sigma-54 dependent transcriptional regulator Regulator
  GCU53_RS07705 thiO 1631057..1632154 (-) 1098 WP_152387103.1 glycine oxidase ThiO -
  GCU53_RS07710 - 1632344..1632907 (+) 564 WP_152387104.1 GspH/FimT family pseudopilin -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58302.53 Da        Isoelectric Point: 9.2263

>NTDB_id=393271 GCU53_RS07695 WP_152389817.1 1627982..1629574(+) (pilS) [Azotobacter salinestris strain KACC 13899]
MPKDSASLAGSVPSGNVLRLYHLYRLVIGLLLVLLISSNLDERLLQVAYPDLFRHGSWLYLILNILVAVLVHHPKPLQLF
SLALVDVILLSGLLYTAGGPSSGIGNLIIVSVAVANILLRGRIGLLIAAVAASGLVYMTFYLGLNRPAAATQYVHAGALG
SLCFAAALLVQSLTRRLQRSESLAERRAAEVADLQALNAQILERMRTGILVLDPQQQVLLANQSAFTLLGNPALVGQPLA
GHCPALVERLKHWQQNPTLRPQDLHTLPHGPLVQPNFVPLTRGGKQSILVFLEDVSQIAQQAQQLKLASLGRLTAGIAHE
IRNPLGAISHAAQLLQESDDLDGPDRRLAQIIQDQSRRMNLVIENVLQLSRRRQAQPQLLDLKYWLHRFASEYRASAAPN
QILHLEIQSGSLKTRMDSNQLIQVLNNLVQNGLRYSAQCHPQGQVWLKLFRDPESDLPVLEVRDDGPGVPAAQIEHIFEP
FFTTESKGTGLGLYLSRELCESNQARLDYRGDEGKGGCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=393271 GCU53_RS07695 WP_152389817.1 1627982..1629574(+) (pilS) [Azotobacter salinestris strain KACC 13899]
CTGCCGAAGGACAGCGCCTCGCTGGCCGGCAGCGTGCCGAGCGGCAACGTCCTGCGCCTGTACCACCTGTACCGGCTGGT
GATCGGCCTGCTGCTGGTACTGCTGATCTCCAGCAATCTGGACGAGCGACTGTTGCAGGTCGCTTACCCGGACCTGTTCC
GCCATGGCAGCTGGCTCTACCTGATCCTGAACATCCTGGTCGCCGTCCTGGTACACCACCCCAAGCCCCTGCAGCTGTTC
AGCCTGGCGCTGGTCGACGTGATCCTGCTCTCGGGTCTGCTCTATACGGCCGGAGGCCCGTCCAGCGGCATCGGCAACCT
GATCATCGTCTCGGTCGCCGTCGCCAACATCCTCCTGCGCGGCCGGATCGGCCTGCTGATCGCCGCCGTGGCGGCCAGCG
GTCTGGTCTACATGACCTTCTACCTGGGACTCAACCGTCCCGCGGCAGCCACCCAGTACGTGCATGCGGGAGCATTGGGC
TCGCTGTGCTTCGCCGCTGCGCTGCTCGTGCAGAGCCTGACCCGCCGCCTGCAGCGCAGCGAATCCCTGGCCGAGCGACG
CGCCGCCGAGGTCGCCGACCTGCAGGCCCTGAACGCGCAGATCCTGGAGCGCATGCGGACCGGCATCCTGGTGCTCGACC
CGCAGCAGCAGGTGCTGCTGGCCAATCAGAGCGCCTTCACCCTGCTCGGCAATCCCGCACTGGTCGGCCAGCCACTCGCC
GGTCACTGCCCTGCGCTGGTCGAACGCCTGAAACATTGGCAGCAGAACCCGACCCTGCGCCCGCAGGATCTGCACACCCT
GCCCCATGGCCCGCTGGTGCAGCCGAACTTCGTTCCGCTCACGCGAGGCGGAAAGCAGAGCATCCTGGTTTTCCTCGAGG
ATGTCTCGCAGATCGCCCAGCAGGCTCAGCAGCTCAAGCTCGCCTCGCTCGGCCGCCTGACCGCCGGCATCGCCCACGAG
ATCCGCAATCCGCTCGGCGCCATCAGCCATGCGGCGCAGTTGCTGCAGGAGTCCGACGACCTCGACGGCCCTGACCGGCG
CCTGGCGCAGATCATCCAGGATCAGTCGCGACGCATGAACTTGGTGATCGAGAACGTGCTGCAGCTGTCGCGCCGGCGCC
AGGCACAACCGCAGCTGCTCGACTTGAAATACTGGCTGCACCGCTTCGCCAGCGAATATCGCGCCTCGGCAGCACCAAAC
CAGATCCTGCATCTGGAAATTCAGAGCGGCTCCCTGAAGACCCGCATGGATTCGAACCAGCTCATCCAGGTTCTCAACAA
CCTGGTCCAGAACGGCCTGCGCTACAGCGCCCAATGTCATCCGCAGGGGCAGGTCTGGCTCAAACTGTTCCGCGATCCGG
AAAGCGACCTGCCGGTGCTCGAGGTGCGCGACGACGGGCCCGGCGTGCCGGCCGCGCAGATCGAGCACATTTTCGAGCCG
TTCTTCACCACGGAAAGCAAGGGAACCGGGCTGGGACTGTACCTCTCCCGCGAGCTGTGCGAGAGCAATCAGGCCCGCCT
CGACTACCGCGGGGACGAAGGCAAGGGCGGCTGCTTCCGCATCACCTTCGCCCATCCGCGCAAGCTGAGCTAG

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

66.019

97.17

0.642

Multiple sequence alignment