Detailed information NTG0392587

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: F8E20_RS21230
• Genome accession: NZ_CP045193
• Coordinates: 4399743..4400543 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain YML0508
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4394743..4405543

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
F8E20_RS21195	gspH	4394899..4395414 (+)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-
F8E20_RS21200	gspI	4395411..4395776 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
F8E20_RS21205	gspJ	4395773..4396369 (+)	597	WP_023302298.1	type II secretion system minor pseudopilin GspJ	-
F8E20_RS21210	gspK	4396366..4397352 (+)	987	WP_004177385.1	type II secretion system minor pseudopilin GspK	-
F8E20_RS21215	gspL	4397339..4398532 (+)	1194	WP_029498035.1	type II secretion system protein GspL	-
F8E20_RS21220	-	4398525..4399010 (+)	486	WP_004145896.1	type II secretion system protein M	-
F8E20_RS21225	-	4399000..4399746 (+)	747	WP_004145897.1	type II secretion system protein N	-
F8E20_RS21230	pilD	4399743..4400543 (+)	801	WP_004145898.1	prepilin peptidase	Machinery gene
F8E20_RS21235	hrpB	4400591..4403020 (-)	2430	WP_023302300.1	ATP-dependent helicase HrpB	-
F8E20_RS21240	thpR	4403093..4403629 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
F8E20_RS21245	sfsA	4403629..4404345 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
F8E20_RS21250	dksA	4404508..4404963 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=392587 F8E20_RS21230 WP_004145898.1 4399743..4400543(+) (pilD) [Klebsiella pneumoniae strain YML0508]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=392587 F8E20_RS21230 WP_004145898.1 4399743..4400543(+) (pilD) [Klebsiella pneumoniae strain YML0508]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425