Detailed information    

insolico Bioinformatically predicted

Overview

Name   comL   Type   Machinery gene
Locus tag   HO397_RS12485 Genome accession   NZ_CP053601
Coordinates   2573121..2573858 (+) Length   245 a.a.
NCBI ID   WP_000197686.1    Uniprot ID   P0AC03
Organism   Escherichia coli BL21     
Function   DNA binding (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2568121..2578858
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HO397_RS12470 (HO397_12470) clpC 2568575..2571148 (-) 2574 WP_001235102.1 ATP-dependent chaperone ClpB Regulator
  HO397_RS12475 (HO397_12475) yfiH 2571278..2572009 (-) 732 WP_000040127.1 purine nucleoside phosphorylase YfiH -
  HO397_RS12480 (HO397_12480) rluD 2572006..2572986 (-) 981 WP_000079100.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  HO397_RS12485 (HO397_12485) comL 2573121..2573858 (+) 738 WP_000197686.1 outer membrane protein assembly factor BamD Machinery gene
  HO397_RS12490 (HO397_12490) raiA 2574129..2574470 (+) 342 WP_000178456.1 ribosome-associated translation inhibitor RaiA -
  HO397_RS12495 (HO397_12495) pheL 2574574..2574621 (+) 48 WP_001386991.1 pheA operon leader peptide PheL -
  HO397_RS12500 (HO397_12500) pheA 2574719..2575879 (+) 1161 WP_000200120.1 bifunctional chorismate mutase/prephenate dehydratase -
  HO397_RS12505 (HO397_12505) tyrA 2575922..2577043 (-) 1122 WP_000225221.1 bifunctional chorismate mutase/prephenate dehydrogenase -
  HO397_RS12510 (HO397_12510) aroF 2577054..2578124 (-) 1071 WP_001168045.1 3-deoxy-7-phosphoheptulonate synthase AroF -
  HO397_RS12515 (HO397_12515) yfiL 2578334..2578699 (+) 366 WP_000976004.1 DUF2799 domain-containing protein -

Sequence

Protein


Download         Length: 245 a.a.        Molecular weight: 27829.40 Da        Isoelectric Point: 6.4874

>NTDB_id=392469 HO397_RS12485 WP_000197686.1 2573121..2573858(+) (comL) [Escherichia coli BL21]
MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQDGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYY
KNADLPLAQAAIDRFIRLNPTHPNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLMENAYRQMQMNAQAEKVAKIIAA
NSSNT

Nucleotide


Download         Length: 738 bp        

>NTDB_id=392469 HO397_RS12485 WP_000197686.1 2573121..2573858(+) (comL) [Escherichia coli BL21]
ATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGT
ACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGC
AACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTAT
AAAAACGCCGATTTGCCGTTAGCACAGGCTGCCATCGATCGTTTTATTCGCCTTAACCCGACCCATCCGAATATCGATTA
TGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGCGTCGATCGTAGCG
ATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACC
ACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGA
ACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATG
CGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCA
AACAGCAGCAATACATAA

Domains

Predicted by InterProScan.

Outer membrane lipoprotein (YfiO)

(28-236)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P0AC03

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comL Neisseria meningitidis MC58

38.525

99.592

0.384
  comL Neisseria gonorrhoeae MS11

37.705

99.592

0.376

Multiple sequence alignment