Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GBA65_RS05620 Genome accession   NZ_CP045121
Coordinates   1097566..1098984 (+) Length   472 a.a.
NCBI ID   WP_166395737.1    Uniprot ID   -
Organism   Rubrobacter marinus strain SCSIO 52915     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1092566..1103984
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBA65_RS05605 (GBA65_05475) - 1092707..1095223 (+) 2517 WP_166395734.1 ATP-dependent Clp protease ATP-binding subunit -
  GBA65_RS05610 (GBA65_05480) - 1095305..1096054 (-) 750 WP_166395735.1 hypothetical protein -
  GBA65_RS05615 (GBA65_05485) - 1096394..1097407 (+) 1014 WP_166395736.1 epimerase -
  GBA65_RS05620 (GBA65_05490) radA 1097566..1098984 (+) 1419 WP_166395737.1 DNA repair protein RadA Machinery gene
  GBA65_RS05625 (GBA65_05495) disA 1098981..1100063 (+) 1083 WP_166395738.1 DNA integrity scanning diadenylate cyclase DisA -
  GBA65_RS26160 - 1100064..1100261 (+) 198 WP_407691043.1 CarD family transcriptional regulator -
  GBA65_RS05630 (GBA65_05500) - 1100186..1100620 (+) 435 WP_207956178.1 CarD family transcriptional regulator -
  GBA65_RS26165 - 1100698..1101355 (+) 658 Protein_1144 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  GBA65_RS26170 ispF 1101418..1101837 (+) 420 Protein_1145 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  GBA65_RS26175 - 1101890..1102597 (+) 708 Protein_1146 glutamate--tRNA ligase -
  GBA65_RS26180 - 1102989..1103105 (+) 117 WP_407691068.1 hypothetical protein -

Sequence

Protein


Download         Length: 472 a.a.        Molecular weight: 49389.54 Da        Isoelectric Point: 5.6697

>NTDB_id=392067 GBA65_RS05620 WP_166395737.1 1097566..1098984(+) (radA) [Rubrobacter marinus strain SCSIO 52915]
MATKSVFTCSNCGHQEPKWLGRCPECGEWSTLVEEAKEDKKVVGFAARAANAKAPGRKAAGATLALNEVRAEREGGRVST
GVGELDRVLGGGIVPGSLVLVGGEPGVGKSTLLLQVMGYLGEGCLMVSGEESPRQVALSARRLGVGDAGFRVLSETDVDV
IEATILEERPRVVVVDSIQTLYSPELTGAPGGVGQVRETAARLMRLAKAEGITVILVGHVTKEGSIAGPRVLEHMVDTVL
QFEGDRYQAFRILRALKNRFGSTNEVGVFEMTGGGMVEVEDPSAFFLSTREGGVPPGVVTVCLLEGTRPMLVEIESLVAP
SPLAVPRRVANGFDTGRVNMLCAVLSRRAGLVLGDQDVYVNVTGGVRVEEPAADLGVALAIASALRDRPVEAGTACFGEV
GLTGDVRFVSGGPRRVTELRKLGFGRIIAPEGASEGSRAGARNGAPNGRAKRKEAAVEEVRTLEEAVGAALS

Nucleotide


Download         Length: 1419 bp        

>NTDB_id=392067 GBA65_RS05620 WP_166395737.1 1097566..1098984(+) (radA) [Rubrobacter marinus strain SCSIO 52915]
ATGGCTACGAAAAGCGTATTTACCTGCTCGAACTGCGGACACCAGGAGCCGAAGTGGCTGGGGCGGTGCCCGGAGTGCGG
GGAGTGGAGCACCCTCGTCGAGGAGGCAAAGGAGGACAAGAAGGTCGTCGGTTTCGCTGCCCGTGCGGCGAACGCGAAGG
CGCCGGGCAGGAAGGCGGCGGGCGCGACGCTGGCGTTGAACGAGGTCCGGGCGGAGCGCGAGGGCGGCAGGGTCTCGACC
GGGGTCGGGGAGTTGGATCGGGTCCTCGGTGGCGGGATCGTGCCGGGCTCGCTCGTCCTCGTCGGCGGGGAGCCCGGCGT
CGGGAAGAGCACGCTCCTGTTGCAGGTGATGGGGTACCTCGGGGAGGGTTGCCTGATGGTCTCGGGGGAGGAGTCCCCGA
GGCAGGTGGCGCTCTCCGCCCGCAGGCTCGGGGTTGGGGACGCCGGGTTCCGGGTGCTCTCGGAGACGGACGTGGACGTG
ATCGAGGCGACGATCCTGGAGGAGCGGCCGAGGGTCGTGGTCGTGGACTCCATCCAGACGCTCTACTCGCCGGAGCTTAC
GGGGGCGCCGGGGGGCGTGGGTCAGGTCCGGGAGACGGCGGCGAGGCTCATGCGGCTCGCGAAGGCGGAGGGGATCACGG
TGATCCTCGTCGGTCACGTCACGAAGGAGGGCTCCATCGCGGGGCCGCGCGTCCTGGAGCACATGGTCGACACGGTGCTC
CAGTTCGAGGGGGACCGCTACCAGGCGTTCCGCATCCTGCGGGCCCTGAAGAACCGCTTCGGCTCGACGAACGAGGTCGG
GGTCTTCGAGATGACGGGCGGGGGGATGGTCGAGGTCGAGGACCCTTCGGCCTTCTTCCTGAGCACGCGCGAGGGCGGGG
TGCCGCCCGGGGTCGTAACGGTGTGCCTCCTCGAGGGGACGCGGCCGATGCTCGTGGAGATCGAGAGCCTCGTGGCGCCG
AGCCCGCTGGCCGTGCCCCGGCGCGTCGCGAACGGGTTCGACACGGGACGGGTGAACATGCTGTGCGCCGTCCTCTCCCG
GCGGGCGGGGCTCGTCCTCGGGGACCAGGACGTGTACGTGAACGTCACCGGCGGGGTTCGGGTGGAGGAGCCGGCGGCGG
ACCTCGGGGTGGCGCTGGCGATCGCCTCGGCCTTGAGAGACCGGCCGGTGGAGGCGGGGACGGCGTGCTTCGGGGAGGTG
GGGCTGACGGGGGACGTGCGCTTCGTCTCCGGCGGGCCGCGGCGCGTGACCGAGCTTCGCAAGCTCGGGTTCGGGCGTAT
AATAGCTCCGGAAGGCGCGTCCGAGGGTTCGAGAGCAGGCGCTCGAAACGGCGCGCCGAACGGTCGGGCGAAGAGGAAGG
AAGCCGCAGTGGAAGAGGTCAGGACGCTCGAGGAGGCCGTGGGGGCGGCGCTAAGTTGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(7-34)

AAA domain (AAA_25)

(88-224)

Subunit ChlI of Mg-chelatase (ChlI)

(350-431)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

51.011

94.28

0.481
  radA Streptococcus pneumoniae Rx1

48.046

92.161

0.443
  radA Streptococcus pneumoniae D39

48.046

92.161

0.443
  radA Streptococcus pneumoniae R6

48.046

92.161

0.443
  radA Streptococcus pneumoniae TIGR4

48.046

92.161

0.443
  radA Streptococcus mitis SK321

48.046

92.161

0.443
  radA Streptococcus mitis NCTC 12261

47.816

92.161

0.441

Multiple sequence alignment