Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   GA645_RS04815 Genome accession   NZ_CP045118
Coordinates   1009418..1011007 (-) Length   529 a.a.
NCBI ID   WP_152220451.1    Uniprot ID   -
Organism   Pseudomonas sp. SCB32     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 1004418..1016007
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GA645_RS04790 pilV 1005144..1005602 (-) 459 WP_152220442.1 type IV pilus modification protein PilV -
  GA645_RS04795 - 1005599..1006087 (-) 489 WP_152220444.1 GspH/FimT family pseudopilin -
  GA645_RS04800 - 1006238..1006750 (-) 513 WP_152220445.1 GspH/FimT family protein -
  GA645_RS04805 thiO 1006939..1008048 (+) 1110 WP_152220447.1 glycine oxidase ThiO -
  GA645_RS04810 pilR 1008062..1009402 (-) 1341 WP_152220449.1 sigma-54 dependent transcriptional regulator Regulator
  GA645_RS04815 pilS 1009418..1011007 (-) 1590 WP_152220451.1 PAS domain-containing sensor histidine kinase Regulator
  GA645_RS04820 - 1010997..1011233 (-) 237 WP_152220453.1 PP0621 family protein -
  GA645_RS04825 - 1011371..1012396 (-) 1026 WP_152220455.1 outer membrane protein assembly factor BamD -
  GA645_RS04830 rluD 1012546..1013505 (+) 960 WP_152220457.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  GA645_RS04835 pgeF 1013502..1014227 (+) 726 WP_152220459.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58372.50 Da        Isoelectric Point: 7.1975

>NTDB_id=392026 GA645_RS04815 WP_152220451.1 1009418..1011007(-) (pilS) [Pseudomonas sp. SCB32]
MAAEGISLTGPGRRVLRLYHLYRLTIGLVLVLLISSNLDGEVLTPAHSELFHIGCWAYLVVNILIAVLMPTPRQLMPLFI
LAALDVLMLSGLFYTAGGIPSGIGTLMVIAVAIANILLRGRIGLLIAALASIGLIYLTFYISLTQAVAFNHYVQAGGLGA
LCFASALLIQALARRQQVIENLAEQRATTVANLEELNALILQRMRTGILVLDDQQHVLLANQSATAMLGSQTLTGRPLVE
HNPDLLSSLQQWQQNPALRPTSLQPHDTAPSVQPAFIPLSRGNEQHTLIFLEDISQIAQQAQQLKLAALGRLTAGIAHEI
RNPLGAISHAAQLLLESEELDPPDRRLTQIIQDQSRRMNLVIENVLQLSRRRQAEPQLLDLKYWLHRFAGEHREGLRAGQ
QLHVETGPGTVQTRIDPHQLTQVLTNLVQNGLRYSAKKNDIGQVWLQLFRDSETDLPVLEVLDDGPGIAAEQRAKLFEPF
FTTESKGTGLGLYISRELCESNQARLDYKSRDEGGSCFRITFAHPRKQS

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=392026 GA645_RS04815 WP_152220451.1 1009418..1011007(-) (pilS) [Pseudomonas sp. SCB32]
GTGGCCGCTGAGGGCATTTCGCTCACCGGGCCGGGACGGCGCGTCCTGCGCCTGTATCACCTGTATCGCCTGACCATCGG
CCTGGTACTGGTACTGCTGATTTCCAGCAACCTTGACGGCGAAGTTCTCACTCCAGCCCATAGCGAGCTGTTCCACATCG
GCTGCTGGGCCTACCTGGTAGTGAACATCCTGATCGCCGTGCTGATGCCAACGCCCCGCCAGTTGATGCCCCTGTTCATA
CTGGCGGCACTGGACGTACTGATGCTCAGCGGGCTGTTCTACACAGCTGGCGGCATCCCGAGCGGTATTGGCACGCTGAT
GGTGATCGCCGTCGCCATCGCCAATATCCTGTTGCGCGGCCGGATCGGCCTGCTGATCGCAGCCCTGGCGTCCATCGGGC
TGATCTACCTGACCTTCTATATCAGCCTTACCCAGGCGGTCGCCTTCAACCATTACGTACAGGCCGGTGGCCTGGGCGCC
CTGTGCTTCGCCTCGGCCCTGCTGATCCAGGCGCTGGCGCGCCGCCAGCAGGTCATCGAGAACCTCGCGGAACAACGGGC
CACCACGGTGGCCAACCTCGAAGAGCTCAACGCCCTGATTCTCCAGCGCATGCGCACGGGCATTTTGGTGCTGGACGATC
AGCAACACGTGCTGCTGGCCAACCAGAGCGCTACTGCCATGCTTGGCAGTCAGACGCTGACCGGGCGCCCACTGGTCGAG
CACAATCCGGACCTGCTCAGCAGCCTGCAGCAGTGGCAGCAGAACCCCGCCCTGCGCCCGACAAGCCTGCAACCCCACGA
CACCGCCCCCAGCGTGCAGCCGGCCTTCATTCCCCTGAGTCGCGGCAATGAACAGCACACCCTGATTTTCCTCGAGGACA
TCTCTCAGATCGCACAACAGGCGCAACAGCTCAAGCTGGCCGCCCTCGGCCGCCTGACTGCCGGCATCGCCCACGAAATC
CGCAACCCCCTGGGCGCCATCAGCCACGCCGCGCAACTGCTCCTGGAGTCCGAAGAACTCGACCCGCCGGACCGGCGGCT
GACGCAGATCATCCAGGACCAGTCGCGCCGGATGAACCTGGTGATCGAGAACGTGCTGCAATTGTCCCGTCGACGCCAGG
CCGAACCACAGCTGCTCGACCTGAAATACTGGCTGCACCGATTCGCCGGCGAACACCGCGAAGGGTTGCGCGCAGGACAG
CAGCTGCATGTCGAAACGGGGCCTGGCACCGTGCAGACGCGCATCGACCCGCACCAGCTGACCCAGGTGCTGACCAACCT
GGTCCAGAATGGGTTACGGTACAGCGCCAAGAAGAACGACATCGGCCAGGTGTGGCTGCAACTGTTCCGCGACAGCGAAA
CTGACCTGCCGGTCCTTGAGGTCCTGGACGATGGCCCGGGCATTGCGGCGGAACAACGGGCCAAGCTGTTCGAGCCGTTC
TTCACCACCGAAAGCAAGGGCACGGGGCTTGGGCTGTACATCTCCCGGGAACTCTGTGAAAGCAACCAGGCGCGCCTGGA
CTATAAATCGCGTGACGAAGGCGGCAGTTGCTTCCGTATAACCTTCGCCCACCCACGCAAACAAAGCTAA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(308-372)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(416-522)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

70.377

100

0.705

Multiple sequence alignment