Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilY1   Type   Machinery gene
Locus tag   GBN68_RS14860 Genome accession   NZ_CP045101
Coordinates   3037781..3041665 (-) Length   1294 a.a.
NCBI ID   WP_234954408.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain AJ210     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3032781..3046665
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBN68_RS14820 (GBN68_14840) - 3033492..3034463 (+) 972 WP_168158649.1 esterase/lipase family protein -
  GBN68_RS14825 (GBN68_14845) rplS 3034523..3034897 (-) 375 WP_004691454.1 50S ribosomal protein L19 -
  GBN68_RS14830 (GBN68_14850) trmD 3035038..3035778 (-) 741 WP_234954404.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  GBN68_RS14835 (GBN68_14855) rimM 3035806..3036354 (-) 549 WP_004978035.1 ribosome maturation factor RimM -
  GBN68_RS14840 (GBN68_14860) rpsP 3036383..3036640 (-) 258 WP_004718284.1 30S ribosomal protein S16 -
  GBN68_RS14845 (GBN68_14865) - 3036786..3037244 (-) 459 WP_234954406.1 type IV pilin protein -
  GBN68_RS14850 (GBN68_14870) - 3037238..3037771 (-) 534 WP_328227704.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GBN68_RS14860 (GBN68_14875) pilY1 3037781..3041665 (-) 3885 WP_234954408.1 PilC/PilY family type IV pilus protein Machinery gene
  GBN68_RS14865 (GBN68_14880) - 3041685..3042491 (-) 807 WP_234954410.1 PilX N-terminal domain-containing pilus assembly protein -
  GBN68_RS14870 (GBN68_14885) - 3042484..3043443 (-) 960 WP_234954412.1 PilW family protein -
  GBN68_RS14875 (GBN68_14890) pilV 3043443..3043961 (-) 519 WP_114836316.1 type IV pilus modification protein PilV Machinery gene
  GBN68_RS14880 (GBN68_14895) - 3043961..3044512 (-) 552 WP_234954414.1 Tfp pilus assembly protein FimT/FimU -
  GBN68_RS14885 (GBN68_14900) ispH 3044678..3045628 (-) 951 WP_004978047.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  GBN68_RS14890 (GBN68_14905) gmk 3045763..3046386 (+) 624 WP_005401338.1 guanylate kinase -

Sequence

Protein


Download         Length: 1294 a.a.        Molecular weight: 139472.57 Da        Isoelectric Point: 5.9021

>NTDB_id=391923 GBN68_RS14860 WP_234954408.1 3037781..3041665(-) (pilY1) [Acinetobacter johnsonii strain AJ210]
MQLKKIQMTILSASLTALMCSSVTQASDLQIYAMPTAGQKTIVMMLDTSGSMENGTGVSGVNRLHALKNGLYDVLNSTDP
KLENVVVGLGQFPLGSNSQSGKILVPAVKLGAPARNGQPESAQRQALRTAVRGLSANGGTPTSHGYTEAASYLMGTRTDD
SMVTLQKKTYYQKYRNEKNQKQCATYESGWSTLTYNSEIRFYKACEVWGTTNVSFSNSDYEFRRGNDNERRYYTIKSNSE
DVVVYSNSTYSGFNDSVEESKEITNGITHYKSPLPIPEKRVSCDGQGVYVLSDGEPNASSDENAELQMKVALAQPTTNVI
SNDFFCDVDSSTLTNPLTGGNTGGAWKCMGEFAKKLFSNANPAGVKIQTAFVGFGSSFNNLNSTASNGNDIRNACKLGSR
TKGDSCSYYQADGLTVNPVSYKNHIGGYGNGGFYTGNAAADVTTSVLNFIDNLGTDPLEPLPTGALSVPVDSLNPNGLQN
LGYLRMLEPNPAQPSLLTWAGNLKKYTMKDGVLVDSTTNVFDTTGNFNTNTKNLWGARSLNDGGKVYVGGTYEMLPMPTT
TKTTALRNVMTDVGSATETALVPLITNNTNGSYDQAKSLLKIVKTENTISKEEVLNTFRNQTTLKDFPISIKLKLLNYLG
FKVDTSLEAIPANDDVSYEGSPYTSMGGIIHSFPVQMTYSGRITATGELATDDITLPDGTILKPRNQSVLYGSMEGGLHI
VDAATGVEQMVFVPAEILNNTVASNALVKSGSGTPAPAHGVDAPWIVDSTYRTTGTYPNSQVNAIKMNVYGGLRMGGNSF
YGLDVLNPTEPKLKFRVGSDQADYTRMGQSWSKPVLIDIRYQGVPTRALVVGGGYDACYEDPRFVLSGSSTANDTSSCTA
KTRAKGNAVYVIDAETGKRLFWVSNTGSDANNADLRHSVVSRISTLDRDGDSYVDHLYFGDLGGQVFRVDMDNYQTKSLT
TTGGTTSTSYSNFGVRTVRLANLATTSTGTAITNGDNPRFYEPVTLTIHDEDSKTFIHVGAASGDRSTPLDVAPTIGRDG
LSPYVALTNRPTNRVYGLFDLDFMKEDLITNTALTGNYTSTTTETDVPTKNRLITRNLTLADLLGNPQTSITENETIYSK
FFSTTAPKHGWYRSLSWSWDTSTTGAEVANNTFRQSGGLKAFEEPIAIGNNLFVPVYDPEGTGVTPQNPCLPRVIGETDR
QQYCLPFGICLTAVGIIDLTREKDSGFRLEATTGGYKNANVVGKGIQGIALANNGGGGGGSNSCGNYTLATLNDGVGNWQ
CTRVLNPTRWFEKR

Nucleotide


Download         Length: 3885 bp        

>NTDB_id=391923 GBN68_RS14860 WP_234954408.1 3037781..3041665(-) (pilY1) [Acinetobacter johnsonii strain AJ210]
ATGCAACTCAAAAAAATCCAAATGACTATACTGAGTGCTTCTTTGACAGCACTGATGTGTAGTAGTGTTACTCAAGCAAG
TGACTTGCAGATTTATGCTATGCCTACAGCGGGTCAGAAGACTATTGTGATGATGTTAGATACGTCAGGCAGTATGGAGA
ATGGAACAGGTGTTAGTGGTGTTAATCGCTTACATGCTTTAAAAAATGGTTTATATGATGTTTTAAACAGTACTGATCCA
AAACTGGAAAATGTTGTTGTTGGTTTGGGGCAGTTCCCTTTAGGAAGTAATAGCCAGAGTGGGAAAATTTTAGTTCCTGC
GGTTAAGCTTGGAGCCCCCGCAAGAAATGGTCAGCCTGAATCTGCTCAACGTCAGGCATTGCGTACTGCTGTGAGGGGGT
TATCTGCAAATGGCGGTACCCCAACATCTCATGGTTATACAGAAGCTGCTTCATATTTAATGGGCACTCGCACAGATGAT
AGTATGGTGACACTACAAAAGAAAACTTACTATCAAAAATATAGAAATGAAAAAAATCAAAAGCAGTGTGCAACATATGA
GTCAGGTTGGTCGACTTTAACGTATAATTCTGAAATTAGATTTTATAAAGCTTGCGAAGTTTGGGGAACTACTAACGTCT
CGTTTTCAAATAGCGATTATGAATTTAGACGAGGCAATGATAACGAACGTAGGTATTACACAATTAAAAGTAATTCAGAG
GATGTAGTTGTTTATAGTAATTCTACATATAGCGGATTCAATGATTCGGTAGAGGAAAGTAAAGAAATTACAAATGGGAT
CACACATTATAAATCACCTTTGCCAATACCTGAGAAAAGAGTTTCATGTGATGGACAAGGAGTTTATGTTTTATCGGATG
GTGAGCCAAATGCTTCATCAGATGAAAATGCCGAGCTGCAAATGAAAGTAGCTTTAGCACAGCCAACTACTAATGTAATT
TCTAATGATTTTTTCTGCGATGTAGATTCGAGTACTTTAACCAATCCATTAACTGGCGGTAATACTGGTGGTGCGTGGAA
ATGTATGGGGGAGTTTGCTAAAAAATTATTTTCTAATGCCAATCCTGCAGGAGTAAAAATCCAAACAGCATTTGTTGGAT
TTGGTAGTAGTTTTAATAATTTAAATTCGACAGCGTCAAACGGTAATGATATTCGTAATGCTTGTAAACTGGGGTCTAGA
ACTAAAGGGGATTCTTGTTCTTATTATCAGGCAGATGGTTTAACTGTAAATCCTGTAAGTTATAAAAACCATATAGGTGG
ATATGGGAATGGCGGTTTTTATACTGGAAATGCTGCTGCTGATGTAACTACAAGCGTACTTAACTTTATTGATAACTTAG
GAACAGATCCATTAGAACCTCTACCAACAGGTGCTTTGTCGGTGCCGGTAGACTCTTTAAATCCAAATGGCCTACAGAAT
TTGGGTTACTTAAGGATGTTAGAGCCAAACCCAGCACAACCGAGCTTGCTGACTTGGGCTGGAAATTTAAAAAAATACAC
GATGAAAGATGGTGTGCTGGTAGATAGCACAACCAATGTTTTTGATACGACAGGTAATTTTAATACAAATACTAAAAATT
TATGGGGTGCACGATCACTGAATGATGGTGGAAAAGTTTATGTTGGAGGGACATATGAAATGTTACCAATGCCAACAACG
ACAAAAACTACAGCTTTAAGAAATGTGATGACGGATGTTGGTAGTGCAACAGAAACAGCCTTAGTTCCATTGATTACAAA
CAATACCAATGGAAGTTATGATCAAGCAAAATCACTTTTAAAAATTGTTAAAACTGAAAATACAATTTCAAAAGAAGAAG
TTTTAAATACATTTAGAAATCAAACCACGCTTAAAGATTTTCCAATTTCAATTAAATTAAAACTTTTAAATTATTTAGGT
TTTAAAGTTGATACAAGTTTGGAGGCCATTCCTGCAAATGATGATGTTAGTTATGAAGGGTCTCCCTATACATCTATGGG
AGGAATTATTCATTCATTCCCTGTTCAAATGACTTATTCTGGACGAATCACTGCAACAGGTGAGCTTGCAACAGATGATA
TTACTCTACCAGATGGAACAATTTTAAAGCCGCGTAATCAGTCCGTCTTGTATGGTTCGATGGAGGGGGGCTTACATATT
GTAGATGCTGCAACGGGCGTCGAACAAATGGTTTTTGTTCCAGCGGAAATTCTAAATAATACTGTTGCGTCTAATGCGTT
AGTTAAAAGTGGCTCGGGAACACCCGCACCTGCGCATGGTGTTGATGCTCCGTGGATTGTGGACTCAACTTATCGCACGA
CAGGAACGTACCCAAACTCTCAAGTAAATGCTATCAAAATGAATGTTTATGGCGGTTTACGTATGGGGGGAAACAGTTTT
TATGGTTTAGATGTATTAAATCCTACAGAGCCCAAGCTTAAATTCAGAGTGGGGTCGGATCAAGCAGACTATACAAGAAT
GGGGCAGTCATGGTCTAAGCCTGTGTTAATTGATATTCGTTATCAAGGTGTACCGACACGAGCTCTAGTTGTAGGTGGTG
GTTATGATGCTTGTTATGAAGATCCTCGTTTTGTTTTATCCGGTTCAAGTACAGCTAATGATACAAGCAGTTGTACAGCA
AAAACACGTGCCAAAGGTAATGCGGTATATGTCATAGATGCGGAAACTGGTAAACGTCTGTTTTGGGTAAGTAATACAGG
CTCTGATGCGAATAATGCTGATTTAAGACATAGCGTAGTTTCACGTATTAGTACTTTAGATCGAGATGGCGATAGTTATG
TAGATCATTTGTACTTTGGTGATCTTGGTGGACAAGTTTTCCGTGTCGATATGGATAACTATCAAACAAAAAGTTTAACA
ACAACAGGCGGAACAACGTCAACGTCATATTCGAACTTTGGTGTGCGTACAGTTCGGTTGGCAAATTTGGCGACGACTAG
CACGGGTACAGCAATTACAAATGGTGATAATCCTCGTTTTTATGAACCAGTAACTTTAACTATTCATGATGAGGATAGTA
AAACATTTATACATGTTGGTGCTGCTTCTGGGGATCGCAGTACGCCTTTAGATGTTGCTCCTACTATAGGACGTGATGGT
TTAAGTCCCTATGTTGCTTTAACAAATCGTCCAACTAATAGAGTTTACGGTTTGTTCGATCTGGATTTTATGAAAGAGGA
TTTGATCACCAATACAGCATTGACTGGAAATTACACTAGTACGACAACAGAAACTGATGTACCAACAAAAAATCGCCTGA
TTACGCGTAACTTAACCTTGGCTGATTTGTTAGGTAATCCGCAAACAAGTATTACTGAAAATGAAACCATTTATAGTAAA
TTCTTTAGCACAACAGCGCCAAAACATGGTTGGTATCGTTCGCTTTCATGGAGTTGGGATACATCTACTACAGGTGCAGA
AGTAGCAAATAATACATTTAGGCAATCAGGTGGTTTAAAAGCATTTGAAGAACCTATAGCAATTGGTAACAACTTATTTG
TTCCTGTATATGATCCAGAAGGAACTGGTGTAACACCTCAGAATCCTTGTTTGCCTCGTGTAATTGGTGAAACTGATCGA
CAACAGTATTGTTTGCCATTTGGGATTTGCTTGACCGCCGTGGGTATTATTGATTTAACTCGTGAGAAAGATTCAGGCTT
TAGACTTGAGGCAACAACAGGTGGATATAAGAATGCTAATGTGGTTGGAAAAGGTATTCAAGGTATAGCGCTTGCCAATA
ATGGTGGCGGTGGCGGTGGTTCAAATAGTTGTGGTAATTATACTTTAGCTACATTGAATGATGGTGTAGGTAATTGGCAG
TGTACTCGTGTGTTGAATCCAACGCGTTGGTTTGAGAAAAGGTAG

Domains

Predicted by InterproScan.

Neisseria PilC beta-propeller domain (T4P_PilY1)

(702-951)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilY1 Acinetobacter baumannii D1279779

46.434

100

0.483

Multiple sequence alignment