Detailed information NTG0391844

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GBN63_RS20435
• Genome accession: NZ_CP045098
• Coordinates: 4553313..4554158 (-)
• Length: 281 a.a.
• NCBI ID: WP_010302946.1
• Uniprot ID: A0AAI9L1D5
• Organism: Pectobacterium carotovorum strain ZM1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4548313..4559158

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GBN63_RS20410 (GBN63_20510)	-	4548504..4549274 (-)	771	WP_239767172.1	ABC transporter ATP-binding protein	-
GBN63_RS20415 (GBN63_20515)	-	4549276..4550313 (-)	1038	WP_039545335.1	FecCD family ABC transporter permease	-
GBN63_RS20420 (GBN63_20520)	-	4550310..4551296 (-)	987	WP_239767174.1	ABC transporter substrate-binding protein	-
GBN63_RS20425 (GBN63_20525)	-	4551697..4552722 (+)	1026	WP_039507896.1	LLM class flavin-dependent oxidoreductase	-
GBN63_RS20430 (GBN63_20530)	ypdK	4553078..4553152 (+)	75	WP_099606229.1	membrane protein YpdK	-
GBN63_RS20435 (GBN63_20535)	pilD	4553313..4554158 (-)	846	WP_010302946.1	prepilin peptidase	Machinery gene
GBN63_RS20440 (GBN63_20540)	-	4554248..4554994 (-)	747	WP_130640256.1	type II secretion system protein N	-
GBN63_RS20445 (GBN63_20545)	gspM	4554984..4555478 (-)	495	WP_010302957.1	type II secretion system protein GspM	-
GBN63_RS20450 (GBN63_20550)	gspL	4555475..4556752 (-)	1278	WP_239767176.1	type II secretion system protein GspL	-
GBN63_RS20455 (GBN63_20555)	gspK	4556749..4557735 (-)	987	WP_039538752.1	type II secretion system minor pseudopilin GspK	-
GBN63_RS20460 (GBN63_20560)	gspJ	4557732..4558382 (-)	651	WP_180782111.1	type II secretion system minor pseudopilin GspJ	-
GBN63_RS20465 (GBN63_20565)	gspI	4558382..4558747 (-)	366	WP_010302972.1	type II secretion system minor pseudopilin GspI	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 31271.14 Da
• Isoelectric Point: 6.4529

>NTDB_id=391844 GBN63_RS20435 WP_010302946.1 4553313..4554158(-) (pilD) [Pectobacterium carotovorum strain ZM1]
MDDLREFAQLFPAWWFGALGVLGLIVGSFLNVVIYRLPIMLERRWRQDIELETGVADSDPDTRYNLWWPPSSCPHCQQAI
AVKDNIPLFSWLWLRGRSRCCHQSVSVQYPLVEVITMLAFLAAGLLWLPGMALWGALILLSFLLVLTVIDIKTLLLPDVL
TLSLLWMGLLFNLSGTFVSLNDAVVGAMAGYLSLWLLYWAFKYATGKEALGYGDFKLLAALGAWLGWQALPNLVLVAALS
GLVVTLIWRGLRKEDTAKPLAFGPWLAIGGVFGVIMNGFNL

Nucleotide

• Length: 846 bp

>NTDB_id=391844 GBN63_RS20435 WP_010302946.1 4553313..4554158(-) (pilD) [Pectobacterium carotovorum strain ZM1]
GTGGACGATTTAAGGGAATTCGCACAGCTGTTTCCGGCGTGGTGGTTTGGGGCGCTCGGCGTGTTGGGGTTGATTGTTGG
CAGTTTTCTGAACGTGGTGATTTACCGCTTGCCGATTATGCTGGAGCGTCGCTGGCGGCAGGACATCGAGCTTGAAACGG
GCGTGGCCGATTCCGATCCTGATACGCGCTACAACCTGTGGTGGCCGCCTTCGTCTTGCCCGCACTGCCAGCAGGCTATT
GCGGTCAAAGATAATATCCCGCTATTCAGCTGGCTGTGGCTGCGCGGCCGCTCCCGCTGCTGCCATCAATCGGTCTCTGT
GCAATATCCGTTAGTGGAAGTCATCACCATGCTGGCTTTTCTGGCGGCAGGTTTGCTCTGGTTGCCCGGTATGGCGCTGT
GGGGGGCGTTGATCCTGCTGTCCTTCCTGTTGGTGCTGACCGTTATTGATATAAAAACGCTGCTGCTGCCGGATGTGCTT
ACGCTGTCGCTTCTGTGGATGGGGTTGCTGTTTAATCTGTCGGGGACATTTGTCTCATTGAATGATGCCGTAGTCGGCGC
GATGGCCGGTTATTTATCTCTGTGGCTACTTTATTGGGCATTCAAATACGCAACGGGCAAAGAAGCGCTGGGGTACGGTG
ATTTTAAGTTGCTGGCTGCGCTGGGGGCTTGGCTTGGCTGGCAGGCATTGCCCAATCTGGTTTTGGTGGCGGCGCTGAGT
GGGCTGGTCGTGACTCTCATCTGGCGCGGGTTGCGTAAAGAGGATACGGCTAAACCGCTGGCCTTTGGCCCCTGGCTGGC
AATCGGCGGGGTGTTCGGCGTGATAATGAACGGATTCAATCTGTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-123)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	51.292	96.441	0.495
pilD	Vibrio campbellii strain DS40M4	47.037	96.085	0.452
pilD	Neisseria gonorrhoeae MS11	46.36	92.883	0.431
pilD	Acinetobacter baumannii D1279779	43.223	97.153	0.42
pilD	Acinetobacter nosocomialis M2	42.857	97.153	0.416